Four novel organogermanium scsquioxidcs with anthraquinonc or naphthalene moiety were synthesized. The structures were characterized by IR NMR and elemental analysis, and their cytotoxicitics wcrc evaluated against hu...Four novel organogermanium scsquioxidcs with anthraquinonc or naphthalene moiety were synthesized. The structures were characterized by IR NMR and elemental analysis, and their cytotoxicitics wcrc evaluated against human chronic mycloid leukemia K562 cell lines. The cytotoxicity could bc improved by the introduction of planar aromatic chromophorc moiety to the parent compound, Ge-132.展开更多
The affinity and mode of interaction of four novel organogermanium sesquioxides with calf thymus DNA（CT-DNA） and two synthetic oligonucleotides, d（AT）22d（AT）22 and d（GC）22d（GC）22, were investigated by a comb...The affinity and mode of interaction of four novel organogermanium sesquioxides with calf thymus DNA（CT-DNA） and two synthetic oligonucleotides, d（AT）22d（AT）22 and d（GC）22d（GC）22, were investigated by a combination of absorption spectroscopy, DNA thermal denaturalization method, viscosity method, fluorometric technique, and competitive binding study with ethidium bromide（EB）. The results show that the organogermanium compounds can interact with DNA by intercalation, the binding ability of the compounds to CT-DNA and the synthetic oligonucleotides was found to be modest（in comparison to the proven intercalators）, With binding constants on the order of 10^3-10^5 L/mol, respectively. Generally, the binding of the organogermanium sesquioxides with naphthalene moiety to DNA is stronger than that of the compounds with anthraquinone moiety. And the compounds with anthraquinone moiety have preference for binding to AT-rich duplexes, whereas the compounds with naphthalene moiety have a little preference for binding to GC-rich duplexes. DNA may be the primary effect target.展开更多
文摘Four novel organogermanium scsquioxidcs with anthraquinonc or naphthalene moiety were synthesized. The structures were characterized by IR NMR and elemental analysis, and their cytotoxicitics wcrc evaluated against human chronic mycloid leukemia K562 cell lines. The cytotoxicity could bc improved by the introduction of planar aromatic chromophorc moiety to the parent compound, Ge-132.
基金Supported by the Natural Science Foundation of Shandong Province, China（No.ZR2011HL003）.
文摘The affinity and mode of interaction of four novel organogermanium sesquioxides with calf thymus DNA（CT-DNA） and two synthetic oligonucleotides, d（AT）22d（AT）22 and d（GC）22d（GC）22, were investigated by a combination of absorption spectroscopy, DNA thermal denaturalization method, viscosity method, fluorometric technique, and competitive binding study with ethidium bromide（EB）. The results show that the organogermanium compounds can interact with DNA by intercalation, the binding ability of the compounds to CT-DNA and the synthetic oligonucleotides was found to be modest（in comparison to the proven intercalators）, With binding constants on the order of 10^3-10^5 L/mol, respectively. Generally, the binding of the organogermanium sesquioxides with naphthalene moiety to DNA is stronger than that of the compounds with anthraquinone moiety. And the compounds with anthraquinone moiety have preference for binding to AT-rich duplexes, whereas the compounds with naphthalene moiety have a little preference for binding to GC-rich duplexes. DNA may be the primary effect target.
