C-repeat binding factors(CBFs)are well-known transcription factors(TFs)that regulate plant cold acclimation.RNA sequencing(RNA-seq)data from diverse plant species provide opportunities to identify other TFs involved i...C-repeat binding factors(CBFs)are well-known transcription factors(TFs)that regulate plant cold acclimation.RNA sequencing(RNA-seq)data from diverse plant species provide opportunities to identify other TFs involved in the cold response.However,this task is challenging because gene gain and loss has led to an intertwined community of co-orthologs and in-paralogs between and within species.Using orthogroup(closely related homologs)analysis,we identified 10,549 orthogroups in five representative eudicots.A phylotranscriptomic analysis of cold-treated seedlings from eudicots identified 35 high-confidence conserved cold-responsive transcription factor orthogroups(CoCoFos).These 35 CoCoFos included the well-known cold-responsive regulators CBFs,HSFC1,ZAT6/10,and CZF1 among others.We used Arabidopsis BBX29 for experimental validation.Expression and genetic analyses showed that cold-induction of BBX29 is CBF-and abscisic acid-independent,and BBX29 is a negative regulator of cold tolerance.Integrative RNA-seq and Cleavage Under Targets and Tagmentation followed by sequencing analyses revealed that BBX29 represses a set of cold-induced TFs(ZAT12,PRR9,RVE1,MYB96,etc.).Altogether,our analysis yielded a library of eudicot CoCoFos and demonstrated that BBX29 is a negative regulator of cold tolerance in Arabidopsis.展开更多
A new kind of regular orthogroups, namely, the LR-normal orthogroups is introduced and investigated. In particular, we will introduce the gearing techniques for fitting semigroup components on a semilattice in the con...A new kind of regular orthogroups, namely, the LR-normal orthogroups is introduced and investigated. In particular, we will introduce the gearing techniques for fitting semigroup components on a semilattice in the construction of LR-normal orthogroups.展开更多
Congruence is a very important aspect in the study of the semigroup theory.In general,the Kernel-trace characterizations,Green's relations and subvarieties are main tools in the consideration of congruences on com...Congruence is a very important aspect in the study of the semigroup theory.In general,the Kernel-trace characterizations,Green's relations and subvarieties are main tools in the consideration of congruences on completely regular semigroups.In this paper,we give one class of congruences on completely regular semigroups with the representation of wreath product of translational hulls on completely simple semigroups.By this new way,the least Clifford semigroup congruences on completely regular semigroups are generalized.展开更多
基金supported by the National Natural Science Foundation of China(31871233)Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(2021C02070-1)the Scientific Research Development Fund of Zhejiang A&F University(2022LFR082).
文摘C-repeat binding factors(CBFs)are well-known transcription factors(TFs)that regulate plant cold acclimation.RNA sequencing(RNA-seq)data from diverse plant species provide opportunities to identify other TFs involved in the cold response.However,this task is challenging because gene gain and loss has led to an intertwined community of co-orthologs and in-paralogs between and within species.Using orthogroup(closely related homologs)analysis,we identified 10,549 orthogroups in five representative eudicots.A phylotranscriptomic analysis of cold-treated seedlings from eudicots identified 35 high-confidence conserved cold-responsive transcription factor orthogroups(CoCoFos).These 35 CoCoFos included the well-known cold-responsive regulators CBFs,HSFC1,ZAT6/10,and CZF1 among others.We used Arabidopsis BBX29 for experimental validation.Expression and genetic analyses showed that cold-induction of BBX29 is CBF-and abscisic acid-independent,and BBX29 is a negative regulator of cold tolerance.Integrative RNA-seq and Cleavage Under Targets and Tagmentation followed by sequencing analyses revealed that BBX29 represses a set of cold-induced TFs(ZAT12,PRR9,RVE1,MYB96,etc.).Altogether,our analysis yielded a library of eudicot CoCoFos and demonstrated that BBX29 is a negative regulator of cold tolerance in Arabidopsis.
文摘A new kind of regular orthogroups, namely, the LR-normal orthogroups is introduced and investigated. In particular, we will introduce the gearing techniques for fitting semigroup components on a semilattice in the construction of LR-normal orthogroups.
基金National Natural Science Foundation of China(No.11671056)General Science Foundation of Shanghai Normal University,China(No.KF201840)。
文摘Congruence is a very important aspect in the study of the semigroup theory.In general,the Kernel-trace characterizations,Green's relations and subvarieties are main tools in the consideration of congruences on completely regular semigroups.In this paper,we give one class of congruences on completely regular semigroups with the representation of wreath product of translational hulls on completely simple semigroups.By this new way,the least Clifford semigroup congruences on completely regular semigroups are generalized.
