Decellularized scaffolds have an irreplaceable role in the development of tissue engineering.Challenges in this field are concentrated on shaping macro-scale constructs and maintaining blood flow.Here,we establish a w...Decellularized scaffolds have an irreplaceable role in the development of tissue engineering.Challenges in this field are concentrated on shaping macro-scale constructs and maintaining blood flow.Here,we establish a whole liver perfused decellularized scaffold cultured with hepatocytes for transplantable bioengineered liver construction.Owing to retained intact gross morphology of liver,geometric structure and biomechanical environment can support whole liver lobe transplantation.Besides,unremoved extracellular matrix(ECM)and vascular net-works can delivery continuous nutrient for cell adhesion.Based on recellularized bioengineering liver scaffold,we have demonstrated its excellent hepatic functions when it orthotopic implanted in acute liver failure(ALF)rats,along with the prolonged survival rate and improved biochemical indicators.These outstanding properties indicate that the orthotopic implantable liver scaffold has broad clinical application prospects in the treatment of ALF and other regeneration diseases.展开更多
基金supported by the National Natural Science Foundation of China (82270646,82100664)the Fundamental Research Funds for the Central Universities (0214-14380510)+6 种基金the Nanjing health science and technology development project for Distinguished Young Scholars (JQX19002)the Natural Science Foundation of Jiangsu Province (BK20190114)Jiangsu Province Postdoctoral Research Funding Program (2021K116B)Key Project supported by Medical Science and technology development Foundation,Nanjing Department of Health (YKK19070)Project of Modern Hospital Management and Development Institute,Nanjing University and Aid project of Nanjing Drum Tower Hospital Health,Education&Research Foundation (NDYG2020047)fundings for Clinical Trials from the Affiliated Drum Tower Hospital,Medical School of Nanjing University (2021-LCYJ-PY-46,2022-LCYJ-PY-35)the Chen Xiao-ping Foundation for the Development of Science and Technology of Hubei Province,China (CXPJJH121001-2021073,CXPJJH122002-019).
文摘Decellularized scaffolds have an irreplaceable role in the development of tissue engineering.Challenges in this field are concentrated on shaping macro-scale constructs and maintaining blood flow.Here,we establish a whole liver perfused decellularized scaffold cultured with hepatocytes for transplantable bioengineered liver construction.Owing to retained intact gross morphology of liver,geometric structure and biomechanical environment can support whole liver lobe transplantation.Besides,unremoved extracellular matrix(ECM)and vascular net-works can delivery continuous nutrient for cell adhesion.Based on recellularized bioengineering liver scaffold,we have demonstrated its excellent hepatic functions when it orthotopic implanted in acute liver failure(ALF)rats,along with the prolonged survival rate and improved biochemical indicators.These outstanding properties indicate that the orthotopic implantable liver scaffold has broad clinical application prospects in the treatment of ALF and other regeneration diseases.
