Bacterial blight(BB), which is caused by Xanthomonas oryzae pv. oryzae(Xoo), is one of the most destructive bacterial diseases of rice(Oryza sativa L.). During plant defense responses, micro RNAs(mi RNAs) play importa...Bacterial blight(BB), which is caused by Xanthomonas oryzae pv. oryzae(Xoo), is one of the most destructive bacterial diseases of rice(Oryza sativa L.). During plant defense responses, micro RNAs(mi RNAs) play important roles in regulating disease resistance. However, the functions of mi RNAs in the interaction between rice and Xoo remain relatively uncharacterized. In this study, we compared the mi RNA profiles of the BB resistant rice introgression line F329 and its susceptible recurrent parent Huang-Hua-Zhan(HHZ) at multiple time points after inoculation with Xoo. A total of 538 known and 312 novel mi RNAs were identified, among which only 17 and 26 were responsive to Xoo infection in F329 and HHZ, respectively. Compared with the expression levels in HHZ, 37 up-regulated and 53 down-regulated mi RNAs were detected in F329. The predicted target genes for the mi RNAs differentially expressed between F329 and HHZ were revealed to be associated with flavonoid synthesis, the reactive oxygen species regulatory pathway, plant hormone signal transduction, defense responses, and growth and development.Additionally, the patterns of interactions between osa-mi R390-3 p, novel_mi R_104, novel_mi R_238,osa-mi R166 k-5 p, osa-mi R529 b, and osa-mi R167 h-3 p and their target genes were further validated by quantitative real-time PCR. Furthermore, we overexpressed osa-mi R167 h-3 p in transgenic plants and proved that this mi RNA positively regulates the resistance of rice to BB. These results provide novel information regarding the mi RNA-based molecular mechanisms underlying the disease resistance of rice. The data presented herein may be useful for engineering rice BB resistance via mi RNAs.展开更多
基金supported by grants from the National Natural Science Foundation of China(31571632 and 31661143009)the CAAS Innovative Team Award,and the Bill&Melinda Gates Foundation(OPP51587)。
文摘Bacterial blight(BB), which is caused by Xanthomonas oryzae pv. oryzae(Xoo), is one of the most destructive bacterial diseases of rice(Oryza sativa L.). During plant defense responses, micro RNAs(mi RNAs) play important roles in regulating disease resistance. However, the functions of mi RNAs in the interaction between rice and Xoo remain relatively uncharacterized. In this study, we compared the mi RNA profiles of the BB resistant rice introgression line F329 and its susceptible recurrent parent Huang-Hua-Zhan(HHZ) at multiple time points after inoculation with Xoo. A total of 538 known and 312 novel mi RNAs were identified, among which only 17 and 26 were responsive to Xoo infection in F329 and HHZ, respectively. Compared with the expression levels in HHZ, 37 up-regulated and 53 down-regulated mi RNAs were detected in F329. The predicted target genes for the mi RNAs differentially expressed between F329 and HHZ were revealed to be associated with flavonoid synthesis, the reactive oxygen species regulatory pathway, plant hormone signal transduction, defense responses, and growth and development.Additionally, the patterns of interactions between osa-mi R390-3 p, novel_mi R_104, novel_mi R_238,osa-mi R166 k-5 p, osa-mi R529 b, and osa-mi R167 h-3 p and their target genes were further validated by quantitative real-time PCR. Furthermore, we overexpressed osa-mi R167 h-3 p in transgenic plants and proved that this mi RNA positively regulates the resistance of rice to BB. These results provide novel information regarding the mi RNA-based molecular mechanisms underlying the disease resistance of rice. The data presented herein may be useful for engineering rice BB resistance via mi RNAs.
