Adipose derived stem cells represent a readily available source of adult stem cells for various biomedical applications. In this study, the proliferation and osteogenic differentiation potential of lanthanum nitrate（...Adipose derived stem cells represent a readily available source of adult stem cells for various biomedical applications. In this study, the proliferation and osteogenic differentiation potential of lanthanum nitrate（La3＋） on human adipose derived mesenchymal stem cells（hADSCs） were investigated for the first time and compared with that of dexamethasone（Dex）. Our results provided evidence that La3＋ at 50 μmol/L concentration promoted proliferation of hADSCs upto 2.4 fold when treated for 21 d in DMEM medium. Treatment of hADSCs with La3＋ containing osteogenic induction medium（α-MEM with ascorbic acid and β-glycerophosphate） for 7 d resulted in higher calcium deposition than that in the presence of Dex（0.1 μmol/L） as shown by Alizarin red S and von Kossa staining. Scanning electron micrographs also showed more extracellular matrix mineralization in the presence of La3＋. After 7 d of treatment with La3＋（10 μmol/L） the expression of RunX2, osteopontin（OP） and osteocalcin（OC） increased 3.4, 5.5 and 2.7 fold respectively. Our results provided evidence that in the presence of La3＋ osteogenic differentiation occurred earlier than that in the presence of Dex.展开更多
基金Project supported by CSIR,New Delhi Provided through the CSIR-network Project-Advanced Drug Delivery System(ADD-CSC0302)
文摘Adipose derived stem cells represent a readily available source of adult stem cells for various biomedical applications. In this study, the proliferation and osteogenic differentiation potential of lanthanum nitrate（La3＋） on human adipose derived mesenchymal stem cells（hADSCs） were investigated for the first time and compared with that of dexamethasone（Dex）. Our results provided evidence that La3＋ at 50 μmol/L concentration promoted proliferation of hADSCs upto 2.4 fold when treated for 21 d in DMEM medium. Treatment of hADSCs with La3＋ containing osteogenic induction medium（α-MEM with ascorbic acid and β-glycerophosphate） for 7 d resulted in higher calcium deposition than that in the presence of Dex（0.1 μmol/L） as shown by Alizarin red S and von Kossa staining. Scanning electron micrographs also showed more extracellular matrix mineralization in the presence of La3＋. After 7 d of treatment with La3＋（10 μmol/L） the expression of RunX2, osteopontin（OP） and osteocalcin（OC） increased 3.4, 5.5 and 2.7 fold respectively. Our results provided evidence that in the presence of La3＋ osteogenic differentiation occurred earlier than that in the presence of Dex.
