Objective To investigate the relationship between the expression and regulation of osteopontin (OPN) and urolithiasis.Methods Normal and stone model rats were treated with 1,25-dihydroxyvitamin D3 (D3), vitamin K, t...Objective To investigate the relationship between the expression and regulation of osteopontin (OPN) and urolithiasis.Methods Normal and stone model rats were treated with 1,25-dihydroxyvitamin D3 (D3), vitamin K, testosterone or estradiol for 7 days, and the expression of osteopontin and its mRNA were detected with immunohistochemistry and Northern blot, respectively. Crystals deposited in rat kidneys were observed with a polarization microscope. The concentrations of crystal components in rat urine were determined.Results The results showed that vitamin K, testosterone and estradiol up-regulated the expression of OPN mRNA and its protein, thus decreasing the precipitation of calcium oxalate in rat kidneys. D3 increased the concentration of calcium in urine, and accelerated the sedimentation of calcium oxalate in rat kidneys.Conclusions These findings indicate that OPN may be an important macromolecule in the normal endogenous inhibition of the formation of urolithiasis. Vitamin K, testosterone and estradiol inhibit the formation of stones via up-regulating the expression of OPN in kidneys, while D3 over dose may accelerate the process.展开更多
目的探讨骨桥蛋白基因rs11730582、rs11439060和rs28357094多态性与糖尿病肾病的关系。方法以2015年7月—2016年3月于长江大学附属第一医院内分泌科住院的湖北地区汉族2型糖尿病病人600例为研究对象。应用质谱法研究骨桥蛋白基因rs11730...目的探讨骨桥蛋白基因rs11730582、rs11439060和rs28357094多态性与糖尿病肾病的关系。方法以2015年7月—2016年3月于长江大学附属第一医院内分泌科住院的湖北地区汉族2型糖尿病病人600例为研究对象。应用质谱法研究骨桥蛋白基因rs11730582、rs11439060和rs28357094多态性在2型糖尿病肾病组(334例,病例组)和2型糖尿病非肾病组(266例,对照组)中的基因频率分布。结果位点rs28357094在病例组和对照组中均表现为纯合子TT,不具有多态性。位点rs11730582和rs11439060的等位基因频率和基因型频率的比较差异无统计学意义。连锁不平衡分析显示rs11730582和rs11439060之间有较强的连锁不平衡,单体型分析发现T-G、C-del G、T-del G 3种单体型,这些单体型的差异比较也无统计学意义。结论骨桥蛋白基因rs11730582、rs11439060和rs28357094多态性与湖北地区汉族居民2型糖尿病合并肾病无关。展开更多
基金ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 39670 72 2 )
文摘Objective To investigate the relationship between the expression and regulation of osteopontin (OPN) and urolithiasis.Methods Normal and stone model rats were treated with 1,25-dihydroxyvitamin D3 (D3), vitamin K, testosterone or estradiol for 7 days, and the expression of osteopontin and its mRNA were detected with immunohistochemistry and Northern blot, respectively. Crystals deposited in rat kidneys were observed with a polarization microscope. The concentrations of crystal components in rat urine were determined.Results The results showed that vitamin K, testosterone and estradiol up-regulated the expression of OPN mRNA and its protein, thus decreasing the precipitation of calcium oxalate in rat kidneys. D3 increased the concentration of calcium in urine, and accelerated the sedimentation of calcium oxalate in rat kidneys.Conclusions These findings indicate that OPN may be an important macromolecule in the normal endogenous inhibition of the formation of urolithiasis. Vitamin K, testosterone and estradiol inhibit the formation of stones via up-regulating the expression of OPN in kidneys, while D3 over dose may accelerate the process.
文摘目的探讨骨桥蛋白基因rs11730582、rs11439060和rs28357094多态性与糖尿病肾病的关系。方法以2015年7月—2016年3月于长江大学附属第一医院内分泌科住院的湖北地区汉族2型糖尿病病人600例为研究对象。应用质谱法研究骨桥蛋白基因rs11730582、rs11439060和rs28357094多态性在2型糖尿病肾病组(334例,病例组)和2型糖尿病非肾病组(266例,对照组)中的基因频率分布。结果位点rs28357094在病例组和对照组中均表现为纯合子TT,不具有多态性。位点rs11730582和rs11439060的等位基因频率和基因型频率的比较差异无统计学意义。连锁不平衡分析显示rs11730582和rs11439060之间有较强的连锁不平衡,单体型分析发现T-G、C-del G、T-del G 3种单体型,这些单体型的差异比较也无统计学意义。结论骨桥蛋白基因rs11730582、rs11439060和rs28357094多态性与湖北地区汉族居民2型糖尿病合并肾病无关。
