目的探讨低密度脂蛋白相关蛋白5(LRP5)基因多态性及突变与绝经后2型糖尿病(type 2diabetic,T2DM)女性伴骨质疏松症(osteoporosis,OP)的关系,为绝经后女性T2DM合并OP的防治提供依据。方法纳入136例新疆绝经后女性,依据T2DM病史或OGTT分为...目的探讨低密度脂蛋白相关蛋白5(LRP5)基因多态性及突变与绝经后2型糖尿病(type 2diabetic,T2DM)女性伴骨质疏松症(osteoporosis,OP)的关系,为绝经后女性T2DM合并OP的防治提供依据。方法纳入136例新疆绝经后女性,依据T2DM病史或OGTT分为NGT组(A组=26例)、骨量异常组(B组=28例)、T2DM组(C组=27例)和T2DM伴骨量异常组(D组=55例);记录年龄、身高等基线资料;检测空腹血糖(FPG)、钙(Ca)、磷(P)等生化指标;采用双能X线(dual energy x-ray,DEXA)法测定骨密度(bone mineral density,BMD);LRP5基因位点的多态性的检测采用飞行时间质谱法。结果 (1)在LRP5基因rs901825位点中,与A组相比,D组基因型分布差异具有统计学意义(P <0.001);在LRP5基因rs7125942位点中,与A组相比,B组基因型分布差异具有统计学意义(P=0.038)。(2)在D组中,LRP5基因rs7125942位点,GC基因型(突变型)的TG高于CC基因型(野生型)[(4.12±0.79)vs.(3.22±1.04),P=0.006]。(3)多元线性回归分析显示:TG(β=0.037,P=0.008)与BMD(L1-4)的水平呈正相关,绝经年限(β=-0.007,P=0.002 5)与BMD(L1-4)的水平呈负相关;绝经年限(β=-0.008,P=0.028)与BMD(股骨颈)的水平呈负相关。结论(1)新疆绝经后女性LRP5基因rs901825、rs7125942位点基因多态性及基因频率分布与骨代谢可能有关;(2)LRP5基因rs7125942位点的基因突变可能与新疆绝经后女性脂代谢有关;(3)在绝经后T2DM女性中,TG的降低和绝经年限的增高是BMD降低的危险因素。展开更多
Background Periodontitis and osteoporosis are one of the frequently encountered diseases in post-menopausal women. Estrogen receptors （ERs） regulated bone metabolism. To investigate the possible effect of ER-elpha ...Background Periodontitis and osteoporosis are one of the frequently encountered diseases in post-menopausal women. Estrogen receptors （ERs） regulated bone metabolism. To investigate the possible effect of ER-elpha （a） gene polymorphisms on bone mineral density （BMD） in pre- and post-menopausal Chinese women with chronic periodontitis （CP）, we provided sufficient quantitative information concerning the correlation between ER gene polymorphisms and BMD in periodontitis. Methods Sixty-five post-menopausal and eighty pre-menopausal CP women, and sixty post-menopausal healthy individuals were recruited in this study. Genomic DNA was extracted from oral mucosa swab sample of each subject by the Chelex-100 method. Determination of the ER-a polymorphisms was performed by polymerese chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique with Xbal and Pvull enzyme. The index for periodontal examination includes clinical attachment loss （CAL） and probing pocket depth （PPD）. BMD was measured by dual-energy X-ray absorptiometry （DEXA）. Results There were no significant differences between the ER-α genotypes of Pvull and Xbal and BMD in post-menopausal and pre-menopausal CP patients, respectively （P 〉0.05）. However, there was association between pre- and post-menopausal CP patients at BMD of lumbar spine L2-L4 （P=0.027） and Ward＇s BMD （P=0.004）. Furthermore, the post-menopausal CP women who carried Pvull TT genotype presented significantly lower Ward＇s BMD than the pre-menopausal CP women （P=-0.007）, meanwhile, the post-menopausal CP women who carried Xbal AA genotype presented significantly lower spine L2-L4 BMD than the pre-menopausal CP women （P=0.003）. Conclusions ER-α gene polymorphisms may be a susceptible indicator for BMD variation of lumbar spine L2-L4 and Ward in Chinese pre- and post-menopausal women patients with CP.展开更多
文摘目的探讨低密度脂蛋白相关蛋白5(LRP5)基因多态性及突变与绝经后2型糖尿病(type 2diabetic,T2DM)女性伴骨质疏松症(osteoporosis,OP)的关系,为绝经后女性T2DM合并OP的防治提供依据。方法纳入136例新疆绝经后女性,依据T2DM病史或OGTT分为NGT组(A组=26例)、骨量异常组(B组=28例)、T2DM组(C组=27例)和T2DM伴骨量异常组(D组=55例);记录年龄、身高等基线资料;检测空腹血糖(FPG)、钙(Ca)、磷(P)等生化指标;采用双能X线(dual energy x-ray,DEXA)法测定骨密度(bone mineral density,BMD);LRP5基因位点的多态性的检测采用飞行时间质谱法。结果 (1)在LRP5基因rs901825位点中,与A组相比,D组基因型分布差异具有统计学意义(P <0.001);在LRP5基因rs7125942位点中,与A组相比,B组基因型分布差异具有统计学意义(P=0.038)。(2)在D组中,LRP5基因rs7125942位点,GC基因型(突变型)的TG高于CC基因型(野生型)[(4.12±0.79)vs.(3.22±1.04),P=0.006]。(3)多元线性回归分析显示:TG(β=0.037,P=0.008)与BMD(L1-4)的水平呈正相关,绝经年限(β=-0.007,P=0.002 5)与BMD(L1-4)的水平呈负相关;绝经年限(β=-0.008,P=0.028)与BMD(股骨颈)的水平呈负相关。结论(1)新疆绝经后女性LRP5基因rs901825、rs7125942位点基因多态性及基因频率分布与骨代谢可能有关;(2)LRP5基因rs7125942位点的基因突变可能与新疆绝经后女性脂代谢有关;(3)在绝经后T2DM女性中,TG的降低和绝经年限的增高是BMD降低的危险因素。
基金This study was supported in part by the a grant from National Natural Science Foundation of China （No. 30572069）.Acknowledgements： The authors are grateful to Prof. LIU Jia-yun, Department of Clinical Molecular Biology, Xijing Hospital, Fourth Military Medical University, for his technical advice.
文摘Background Periodontitis and osteoporosis are one of the frequently encountered diseases in post-menopausal women. Estrogen receptors （ERs） regulated bone metabolism. To investigate the possible effect of ER-elpha （a） gene polymorphisms on bone mineral density （BMD） in pre- and post-menopausal Chinese women with chronic periodontitis （CP）, we provided sufficient quantitative information concerning the correlation between ER gene polymorphisms and BMD in periodontitis. Methods Sixty-five post-menopausal and eighty pre-menopausal CP women, and sixty post-menopausal healthy individuals were recruited in this study. Genomic DNA was extracted from oral mucosa swab sample of each subject by the Chelex-100 method. Determination of the ER-a polymorphisms was performed by polymerese chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique with Xbal and Pvull enzyme. The index for periodontal examination includes clinical attachment loss （CAL） and probing pocket depth （PPD）. BMD was measured by dual-energy X-ray absorptiometry （DEXA）. Results There were no significant differences between the ER-α genotypes of Pvull and Xbal and BMD in post-menopausal and pre-menopausal CP patients, respectively （P 〉0.05）. However, there was association between pre- and post-menopausal CP patients at BMD of lumbar spine L2-L4 （P=0.027） and Ward＇s BMD （P=0.004）. Furthermore, the post-menopausal CP women who carried Pvull TT genotype presented significantly lower Ward＇s BMD than the pre-menopausal CP women （P=-0.007）, meanwhile, the post-menopausal CP women who carried Xbal AA genotype presented significantly lower spine L2-L4 BMD than the pre-menopausal CP women （P=0.003）. Conclusions ER-α gene polymorphisms may be a susceptible indicator for BMD variation of lumbar spine L2-L4 and Ward in Chinese pre- and post-menopausal women patients with CP.
