由于芦荟具有医疗、美容、保健、食用等多种功能,近年来成为研究热点.海藻糖对生物体及生物分子具有良好的非特异性保护作用,并已在食品、化妆品、分子生物学领域得到了广泛应用.将海藻糖合成酶基因转入芦荟,对于改良芦荟的商业价值有...由于芦荟具有医疗、美容、保健、食用等多种功能,近年来成为研究热点.海藻糖对生物体及生物分子具有良好的非特异性保护作用,并已在食品、化妆品、分子生物学领域得到了广泛应用.将海藻糖合成酶基因转入芦荟,对于改良芦荟的商业价值有重要意义.目前为止,关于芦荟转化还没有报道.本实验将含有海藻糖合成酶otsA基因的pBI-otsA质粒与含有bar基因的pAHC25质粒混合包埋金粉子弹,利用基因枪介导的共转化法轰击预培养4～5 d的芦荟茎部外植体,经在含1.0～2.0 mg/ L glufosinate筛选剂的改良MS培养基上多次筛选和分化,获得了一定数量的抗性再生芽,对移栽成活的抗性再生植株进行了PCR检测.结果表明,otsA基因、nptII基因、bar基因已经整合到芦荟基因组中,首次获得了芦荟转基因植株.展开更多
The enzyme Ots A（trehalose-P synthase） plays a critical role in the biosynthesis of trehalose, which is a nonreducing disaccharide that plays important functions in many organisms. By using light scattering techniqu...The enzyme Ots A（trehalose-P synthase） plays a critical role in the biosynthesis of trehalose, which is a nonreducing disaccharide that plays important functions in many organisms. By using light scattering technique, we discovered that Ots A in Arthrobacter strain A3 polymerized in the presence of divalent metal ions（Mg2＋ or Ca2＋）, and the kinetics of the assembly was dependent on their concentrations. We identified potential compounds that can affect the kinetics of the polymerization, particularly, heparin, which acts as a very promising inhibitor of the polymerization. The Ots A assembly turns out to be a very delicate process that is finely regulated by p H. Ots A may be in the polymerized form at physiological pH in vivo, suggesting a more complicated mechanism of the enzyme. These unique properties of Ots A provide novel insights into the molecular mechanism of the biosynthesis of trehalose.展开更多
文摘由于芦荟具有医疗、美容、保健、食用等多种功能,近年来成为研究热点.海藻糖对生物体及生物分子具有良好的非特异性保护作用,并已在食品、化妆品、分子生物学领域得到了广泛应用.将海藻糖合成酶基因转入芦荟,对于改良芦荟的商业价值有重要意义.目前为止,关于芦荟转化还没有报道.本实验将含有海藻糖合成酶otsA基因的pBI-otsA质粒与含有bar基因的pAHC25质粒混合包埋金粉子弹,利用基因枪介导的共转化法轰击预培养4～5 d的芦荟茎部外植体,经在含1.0～2.0 mg/ L glufosinate筛选剂的改良MS培养基上多次筛选和分化,获得了一定数量的抗性再生芽,对移栽成活的抗性再生植株进行了PCR检测.结果表明,otsA基因、nptII基因、bar基因已经整合到芦荟基因组中,首次获得了芦荟转基因植株.
基金Supported by the National Natural Science Foundation of China(21305056)the Open Fund of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(T151402)
文摘The enzyme Ots A（trehalose-P synthase） plays a critical role in the biosynthesis of trehalose, which is a nonreducing disaccharide that plays important functions in many organisms. By using light scattering technique, we discovered that Ots A in Arthrobacter strain A3 polymerized in the presence of divalent metal ions（Mg2＋ or Ca2＋）, and the kinetics of the assembly was dependent on their concentrations. We identified potential compounds that can affect the kinetics of the polymerization, particularly, heparin, which acts as a very promising inhibitor of the polymerization. The Ots A assembly turns out to be a very delicate process that is finely regulated by p H. Ots A may be in the polymerized form at physiological pH in vivo, suggesting a more complicated mechanism of the enzyme. These unique properties of Ots A provide novel insights into the molecular mechanism of the biosynthesis of trehalose.
