The article is devoted to the study of the issues of determining the patterns of changes in air pressure along the length of a pneumatic transmission pipeline for raw cotton at different flow parameters and different ...The article is devoted to the study of the issues of determining the patterns of changes in air pressure along the length of a pneumatic transmission pipeline for raw cotton at different flow parameters and different pipeline diameters. Theoretical studies have proved the reduction of static and total pressure along the line of pneumatic cotton transportation. The dependence of the pressure change on the diameter of the transport line and the aerodynamic drag of the pipeline is obtained. The results obtained are recommended for use in the design of raw cotton pneumatic transport systems.展开更多
【目的】建立快速检测棉花根际细菌的Taq Man探针实时荧光定量PCR,对新疆棉花不同生育时期根际细菌数量进行检测以及时空动态分析。【方法】根据棉花根际细菌16S r DNA基因序列,设计、合成特异性引物和Tag Man探针,以构建的重组质粒作...【目的】建立快速检测棉花根际细菌的Taq Man探针实时荧光定量PCR,对新疆棉花不同生育时期根际细菌数量进行检测以及时空动态分析。【方法】根据棉花根际细菌16S r DNA基因序列,设计、合成特异性引物和Tag Man探针,以构建的重组质粒作为阳性标准品,建立标准曲线,并对实际样品进行检测。【结果】新疆棉花不同生育时期根际细菌数量变化不尽相同:库尔勒、阿拉尔和哈密的棉花根际细菌数量变化趋势在苗期至花期之间相同,而花期至絮期之间变化各不相同;石河子、乌苏和图木舒克的棉花在整个生育时期内根际细菌数量变化趋势基本相同;精河的棉花根际细菌从苗期开始缓慢增加,蕾期至花期之间变化不大,花期之后快速增加。新疆不同采样地点棉花根际细菌数量变化也不尽相同:根际细菌数量变化趋势是东疆、南疆、北疆依次减少。其中,棉花根际细菌数量最多的是东疆地区的哈密,吐絮期达到1.7×10~7copies/g(FRW),最少的是北疆地区的精河,苗期仅为8.5×10~4copies/g(FRW)。【结论】建立的Taq Man探针实时荧光定量PCR方法可以快速检测棉花根际细菌的数量,新疆棉花根际细菌数量在时间和空间上变化不尽相同,其中,根际细菌数量最多的是哈密的吐絮期,最少的是精河的苗期。展开更多
文摘The article is devoted to the study of the issues of determining the patterns of changes in air pressure along the length of a pneumatic transmission pipeline for raw cotton at different flow parameters and different pipeline diameters. Theoretical studies have proved the reduction of static and total pressure along the line of pneumatic cotton transportation. The dependence of the pressure change on the diameter of the transport line and the aerodynamic drag of the pipeline is obtained. The results obtained are recommended for use in the design of raw cotton pneumatic transport systems.