Background: The ovarian tumors with functioning stroma are defined by the morphological presence of endocrine active cells in stroma, and the clinical, biochemical or pathological evidence of endocrine function. Case ...Background: The ovarian tumors with functioning stroma are defined by the morphological presence of endocrine active cells in stroma, and the clinical, biochemical or pathological evidence of endocrine function. Case Report: The ovarian endometrioid adenocarcinoma with functioning stroma accompanied with endometrial endometrioid adenocarcinoma was found in 64-year-old post-menopausal woman complaining abnormal genital bleeding and mammary distention. Her preoperative serum 17?-estradiol level was high (53.2 pg/ml) while human chorionic gonadotropin (hCG) level was within normal limit. Her right ovary with 8.8 × 5.3 cm in size and tan-yellow in color mostly consisted of solid tumor. Histologically, tumor was composed of estrogen receptor (ER)- and progesterone receptor (PgR)-positive, and androgen receptor (AR)-negative cancerous endometrial cells with aggregates of vacuolated foamy stromal cells resembling luteinized cells. These stromal cells contained lipid droplets, and was immunopositive for α-inhibin and 17?-estradiol. After surgery, serum 17?-estradiol level decreased and became normal (14.2 pg/ml). These findings indicate the production of steroid hormone (17?-estradiol) from the foamy stromal cells and may be correlated with the clinical symptoms. Furthermore, ER- and PgR-positive endometrial endometrioid adenocarcinoma developed synchronously. However, ovary and uterus were totally immunonegative for human chorionic gonadotropin (hCG). Four other cases from the literature including ours are reviewed. Conclusion: Cancer cells were positive for ER and PgR in both ovary and uterus responded to steroid hormone produced by foamy stromal cells, which played a role in proliferation and progression of ovarian and endometrial endometrioid adenocarcinoma, respectively.展开更多
A 62-year-old woman with a history of curatively treated mucinous ovarian cancer, presented with dyspnea, anorexia and right-upper-quadrant pain at consultation with her general practitioner. A CT scan revealed severa...A 62-year-old woman with a history of curatively treated mucinous ovarian cancer, presented with dyspnea, anorexia and right-upper-quadrant pain at consultation with her general practitioner. A CT scan revealed several lymph node metastases in lungs and abdomen as well as a tumor in Morrison’s pouch and biopsy revealed renal cell carcinoma. Therefore, she was referred to Department of Urology. The multidisciplinary team could not immediately reject that there could be an exophytic tumor in the right kidney but discrepancy between histology and imaging, led to several biopsies including laparoscopic procedure. Re-examination of the primary ovarian cancer showed that one percent was classified as clear cell carcinoma. The final diagnose was metastatic clear cell ovarian carcinoma. The patient was terminal and suffered of cachexia and pleural effusion. The patient deceased four months after first consultation.展开更多
The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cell...The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment.Approximate 1 300 protein spots were resolved in the two ranges of pI（3—10）.The spots which have 10-fold difference in light density were digested by in-gel（enzyme）,identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and finally detected by Profound and Mascot search engine in IDI databases.Seven proteins with obviously altered abundances were detected specifically,including four up-regulated protein spots and three down-regulated protein spots.The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.展开更多
目的研究Rac1蛋白在人卵巢浆液性腺癌组织中的表达及其对卵巢癌细胞株SKOV3恶性增殖和迁移的影响。方法 Western blot检测人卵巢浆液性腺癌组织、卵巢良性浆液性囊腺瘤组织及卵巢正常组织中Rac1蛋白的表达水平;构建Rac1表达缺失的细胞株...目的研究Rac1蛋白在人卵巢浆液性腺癌组织中的表达及其对卵巢癌细胞株SKOV3恶性增殖和迁移的影响。方法 Western blot检测人卵巢浆液性腺癌组织、卵巢良性浆液性囊腺瘤组织及卵巢正常组织中Rac1蛋白的表达水平;构建Rac1表达缺失的细胞株SKOV3-Rac1i;分别用细胞划痕实验、MTT及平板克隆实验检测SKOV3-Rac1i细胞迁移能力、细胞增殖及克隆形成能力。结果卵巢浆液性腺癌组织中Rac1蛋白表达水平显著高于卵巢良性浆液性囊腺瘤组织[(0.89±0.17)vs(0.69±0.24),P<0.05]和卵巢正常组织[(0.89±0.17)vs(0.72±0.12),P<0.05];与亲代细胞相比,SKOV3-Rac1i细胞表现出迁移能力减弱(P<0.05)和增殖能力明显受限(P<0.05)。结论 Rac1在人卵巢浆液性腺癌组织中呈高表达,其可能是通过影响细胞迁移能力及细胞的增殖而促进卵巢癌细胞的恶性生物行为。展开更多
文摘Background: The ovarian tumors with functioning stroma are defined by the morphological presence of endocrine active cells in stroma, and the clinical, biochemical or pathological evidence of endocrine function. Case Report: The ovarian endometrioid adenocarcinoma with functioning stroma accompanied with endometrial endometrioid adenocarcinoma was found in 64-year-old post-menopausal woman complaining abnormal genital bleeding and mammary distention. Her preoperative serum 17?-estradiol level was high (53.2 pg/ml) while human chorionic gonadotropin (hCG) level was within normal limit. Her right ovary with 8.8 × 5.3 cm in size and tan-yellow in color mostly consisted of solid tumor. Histologically, tumor was composed of estrogen receptor (ER)- and progesterone receptor (PgR)-positive, and androgen receptor (AR)-negative cancerous endometrial cells with aggregates of vacuolated foamy stromal cells resembling luteinized cells. These stromal cells contained lipid droplets, and was immunopositive for α-inhibin and 17?-estradiol. After surgery, serum 17?-estradiol level decreased and became normal (14.2 pg/ml). These findings indicate the production of steroid hormone (17?-estradiol) from the foamy stromal cells and may be correlated with the clinical symptoms. Furthermore, ER- and PgR-positive endometrial endometrioid adenocarcinoma developed synchronously. However, ovary and uterus were totally immunonegative for human chorionic gonadotropin (hCG). Four other cases from the literature including ours are reviewed. Conclusion: Cancer cells were positive for ER and PgR in both ovary and uterus responded to steroid hormone produced by foamy stromal cells, which played a role in proliferation and progression of ovarian and endometrial endometrioid adenocarcinoma, respectively.
文摘A 62-year-old woman with a history of curatively treated mucinous ovarian cancer, presented with dyspnea, anorexia and right-upper-quadrant pain at consultation with her general practitioner. A CT scan revealed several lymph node metastases in lungs and abdomen as well as a tumor in Morrison’s pouch and biopsy revealed renal cell carcinoma. Therefore, she was referred to Department of Urology. The multidisciplinary team could not immediately reject that there could be an exophytic tumor in the right kidney but discrepancy between histology and imaging, led to several biopsies including laparoscopic procedure. Re-examination of the primary ovarian cancer showed that one percent was classified as clear cell carcinoma. The final diagnose was metastatic clear cell ovarian carcinoma. The patient was terminal and suffered of cachexia and pleural effusion. The patient deceased four months after first consultation.
文摘The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment.Approximate 1 300 protein spots were resolved in the two ranges of pI（3—10）.The spots which have 10-fold difference in light density were digested by in-gel（enzyme）,identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and finally detected by Profound and Mascot search engine in IDI databases.Seven proteins with obviously altered abundances were detected specifically,including four up-regulated protein spots and three down-regulated protein spots.The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.
文摘目的研究Rac1蛋白在人卵巢浆液性腺癌组织中的表达及其对卵巢癌细胞株SKOV3恶性增殖和迁移的影响。方法 Western blot检测人卵巢浆液性腺癌组织、卵巢良性浆液性囊腺瘤组织及卵巢正常组织中Rac1蛋白的表达水平;构建Rac1表达缺失的细胞株SKOV3-Rac1i;分别用细胞划痕实验、MTT及平板克隆实验检测SKOV3-Rac1i细胞迁移能力、细胞增殖及克隆形成能力。结果卵巢浆液性腺癌组织中Rac1蛋白表达水平显著高于卵巢良性浆液性囊腺瘤组织[(0.89±0.17)vs(0.69±0.24),P<0.05]和卵巢正常组织[(0.89±0.17)vs(0.72±0.12),P<0.05];与亲代细胞相比,SKOV3-Rac1i细胞表现出迁移能力减弱(P<0.05)和增殖能力明显受限(P<0.05)。结论 Rac1在人卵巢浆液性腺癌组织中呈高表达,其可能是通过影响细胞迁移能力及细胞的增殖而促进卵巢癌细胞的恶性生物行为。
