Development of synthetic bone substitutes has arisen as a major research interest in the need to find an alternative to autologous bone grafts.Using an ovine model,the present pre-clinical study presents a synthetic b...Development of synthetic bone substitutes has arisen as a major research interest in the need to find an alternative to autologous bone grafts.Using an ovine model,the present pre-clinical study presents a synthetic bone graft(BonelikeVR)in combination with a cellular system as an alternative for the regeneration of non-critical defects.The association of biomaterials and cell-based therapies is a promising strategy for bone tissue engineering.Mesenchymal stem cells(MSCs)from human dental pulp have demonstrated both in vitro and in vivo to interact with diverse biomaterial systems and promote mineral deposition,aiming at the reconstruction of osseous defects.Moreover,these cells can be found and isolated from many species.Non-critical bone defects were treated with BonelikeVR with or without MSCs obtained from the human dental pulp.Results showed that BonelikeVR and MSCs treated defects showed improved bone regeneration compared with the defects treated with BonelikeVR alone.Also,it was observed that the biomaterial matrix was reabsorbed and gradually replaced by new bone during the healing process.We therefore propose this combination as an efficient binomial strategy that promotes bone growth and vascularization in non-critical bone defects.展开更多
基金This research was supported by Programa Operacional Regional do Norte(ON.2–O Novo Norte),QREN,FEDER with the project‘iBone Therapies:Terapias inovadoras para a regenerac¸~ao o´ssea’,ref.NORTE-01-0247-FEDER-003262,and by the programme COMPETE–Programa Operacional Factores de Competitividade,Projects PEst-OE/AGR/UI0211/2011 and PEst-C/EME/UI0285/2013 funding from FCT.This research was also supported by Programa Operacional Competitividade e Internacionalizac¸~ao(P2020),Fundos Europeus Estruturais e de Investimento(FEEI)and FCT with the project‘BioMate—A novel bio-manufacturing system to produce bioactive scaffolds for tissue engineering’with reference PTDC/EMS-SIS/7032/2014 and by COMPETE 2020,from ANI—Projectos ID&T Empresas em Copromoc¸~ao,Programas Operacionais POCI,by the project‘insitu.Biomas-Reinvent biomanufacturing systems by using an usability approach for in situ clinic temporary implants fabrication’with the reference POCI-01-0247-FEDER-017771.The research was also supported by the research project‘BEPIM III–Microdispositivos me´dicos com capacidades osteintegradoras por micoPIM’,with the reference POCI-01-0247-FEDER-017935,from Fundo Europeu de Desenvolvimento Regional(FEDER),by the Programa Operacional da Competitividade&Internacionalizac¸~ao.Ana Rita Caseiro(SFRH/BD/101174/2014)acknowledges FCT,for financial support.
文摘Development of synthetic bone substitutes has arisen as a major research interest in the need to find an alternative to autologous bone grafts.Using an ovine model,the present pre-clinical study presents a synthetic bone graft(BonelikeVR)in combination with a cellular system as an alternative for the regeneration of non-critical defects.The association of biomaterials and cell-based therapies is a promising strategy for bone tissue engineering.Mesenchymal stem cells(MSCs)from human dental pulp have demonstrated both in vitro and in vivo to interact with diverse biomaterial systems and promote mineral deposition,aiming at the reconstruction of osseous defects.Moreover,these cells can be found and isolated from many species.Non-critical bone defects were treated with BonelikeVR with or without MSCs obtained from the human dental pulp.Results showed that BonelikeVR and MSCs treated defects showed improved bone regeneration compared with the defects treated with BonelikeVR alone.Also,it was observed that the biomaterial matrix was reabsorbed and gradually replaced by new bone during the healing process.We therefore propose this combination as an efficient binomial strategy that promotes bone growth and vascularization in non-critical bone defects.
