Limosilactobacillus reuteri is a microbe intricately linked to humans and animal health.A thorough assessment of its safety and potential benefits is imperative prior to its application in human and animals.In this in...Limosilactobacillus reuteri is a microbe intricately linked to humans and animal health.A thorough assessment of its safety and potential benefits is imperative prior to its application in human and animals.In this investigation,we performed a comprehensive analysis encompassing genome sequencing,genomic analysis,and phenotypic characterization of L.reuteri Q35,an exceptionally proficient producer of reuterin.The whole genome sequencing results showed that the complete genome sequence spans 2145158 bp with a GC content of 38.9%and encompasses 2121 genes.Initial identification of antibiotic-resistant genes,virulence factors,and toxin-coding genes in the genome substantiated the strain’s low-risk status.Subsequent tests for antibiotic resistance,acute oral toxicology,and hemolysis further confirmed its elevated safety level.The genome of L.reuteri Q35 was found to contain genes associated with adhesion and stress tolerance.Following exposure to artificial gastric juice and bile salt,the strain exhibited a higher survival rate and demonstrated a strong scavenging ability for hydroxyl free radicals in antioxidant capacity tests.These findings suggested that L.reuteri Q35 possesses unique probiotic properties.Additionally,the genome of strain Q35 harbors three truncated oxaloyl-CoA decarboxylase genes(oxc1,oxc2 and oxc3),overexpression of which resulted in a significant increase in ammonium oxalate degradation from 29.5%to 48.8%.These findings highlight that L.reuteri Q35 exhibits both favorable safety characteristics alongside beneficial properties,making it a promising candidate for treating metabolic disorders such as hyperoxaluria.展开更多
Catalytic ozonation of aqueous solutions of oxalic acid was examined in the presence of graphite-supported platinum catalysts.The catalytic activity of graphite was significantly enhanced by loading platinum.The remov...Catalytic ozonation of aqueous solutions of oxalic acid was examined in the presence of graphite-supported platinum catalysts.The catalytic activity of graphite was significantly enhanced by loading platinum.The removal efficiency of oxalic acid was 3.0%,47.6%and 99.3%for ozonation alone,graphite catalytic ozonation and Pt/graphite catalytic ozonation in 30 min under the experimental condi-tion,respectively.The influence of support pretreatment,solvent,impregnation time,platinum loading amount and reduction temperature on the activity of Pt/graphite catalyst was investigated.The pretreatment of graphite support had no effect on activity improvement of Pt/graphite catalyst.Solvent and impregnation time also had no great effect on the activity.Platinum loading amount and reduction temperature influenced the catalyst activity significantly.The optimal catalytic performance of Pt/graphite was obtained when 1.0%platinum loading and 623 K of reduction temperature was adopted.The Pt/graphite catalyst was used for five times with no significant decrease in its activity and more than 90%oxalic acid removal was obtained.展开更多
文摘Limosilactobacillus reuteri is a microbe intricately linked to humans and animal health.A thorough assessment of its safety and potential benefits is imperative prior to its application in human and animals.In this investigation,we performed a comprehensive analysis encompassing genome sequencing,genomic analysis,and phenotypic characterization of L.reuteri Q35,an exceptionally proficient producer of reuterin.The whole genome sequencing results showed that the complete genome sequence spans 2145158 bp with a GC content of 38.9%and encompasses 2121 genes.Initial identification of antibiotic-resistant genes,virulence factors,and toxin-coding genes in the genome substantiated the strain’s low-risk status.Subsequent tests for antibiotic resistance,acute oral toxicology,and hemolysis further confirmed its elevated safety level.The genome of L.reuteri Q35 was found to contain genes associated with adhesion and stress tolerance.Following exposure to artificial gastric juice and bile salt,the strain exhibited a higher survival rate and demonstrated a strong scavenging ability for hydroxyl free radicals in antioxidant capacity tests.These findings suggested that L.reuteri Q35 possesses unique probiotic properties.Additionally,the genome of strain Q35 harbors three truncated oxaloyl-CoA decarboxylase genes(oxc1,oxc2 and oxc3),overexpression of which resulted in a significant increase in ammonium oxalate degradation from 29.5%to 48.8%.These findings highlight that L.reuteri Q35 exhibits both favorable safety characteristics alongside beneficial properties,making it a promising candidate for treating metabolic disorders such as hyperoxaluria.
基金This work was supported by the National Natural Science Foundation of China(Grant No.50378028).
文摘Catalytic ozonation of aqueous solutions of oxalic acid was examined in the presence of graphite-supported platinum catalysts.The catalytic activity of graphite was significantly enhanced by loading platinum.The removal efficiency of oxalic acid was 3.0%,47.6%and 99.3%for ozonation alone,graphite catalytic ozonation and Pt/graphite catalytic ozonation in 30 min under the experimental condi-tion,respectively.The influence of support pretreatment,solvent,impregnation time,platinum loading amount and reduction temperature on the activity of Pt/graphite catalyst was investigated.The pretreatment of graphite support had no effect on activity improvement of Pt/graphite catalyst.Solvent and impregnation time also had no great effect on the activity.Platinum loading amount and reduction temperature influenced the catalyst activity significantly.The optimal catalytic performance of Pt/graphite was obtained when 1.0%platinum loading and 623 K of reduction temperature was adopted.The Pt/graphite catalyst was used for five times with no significant decrease in its activity and more than 90%oxalic acid removal was obtained.
