Objective To investigate the effects of hemoglobin (Hb) on serum nitric oxide (ON) concentrationand hemodynamics pattern changes in rats with cirrhosis. Methods Cirrhosis model was induced in male SDrats by injection ...Objective To investigate the effects of hemoglobin (Hb) on serum nitric oxide (ON) concentrationand hemodynamics pattern changes in rats with cirrhosis. Methods Cirrhosis model was induced in male SDrats by injection of 60% CCl4 oily solution subcutaneously. Cirrhotic rats were treated with erythropoietin(100U/kg) injected subcutaneously for 2 weeks. Mean arterial pressure (MAP), cardiac output (CO), cardiac index(CI), splanchnic vascular resistance (SVR), splanchnic blood flow (SBF) and serum NO concentration weredetermined in erythropoietin - treated, erythropoietin - untreated cirrhotic rats and controls by using 57Co - labelledmicrosphere technique and a fluorometric assay, respectively. In addition, blood Hb levels were also measured inthe 3 groups. Results Untreated cirrhotic rats had significantly lower MAP, SVR, Hb and higher Co, CI, SBFand NO concentration than those of the controls (P<0.01). In treated cirrhotic rats, erythropoietin significantlyattenuated the increase of CO, CI, SBF, NO concentration and the decrease of MAP and SVR. In cirrhotic rats,opoetin beta in subcutaneous dose of 100U·kg-1·d-1 induced a markedly increment of blood Hb levels anddecrement of NO concentration in comparison with untreated cirrhotic rats (181±11g/L vs 120±15g/L,1.14±0.62μmol/L vs 4.20±1.25μmol/L). Conclusion The endogenous NO may play an important role in thechanges of hemodynamics pattern in cirrhosis, and hyperdynamic circulatory status in rats with cirrhosis might beameliorated by inactivation of overproduced NO by increasing hemoglobin with erythropoietin.展开更多
文摘Objective To investigate the effects of hemoglobin (Hb) on serum nitric oxide (ON) concentrationand hemodynamics pattern changes in rats with cirrhosis. Methods Cirrhosis model was induced in male SDrats by injection of 60% CCl4 oily solution subcutaneously. Cirrhotic rats were treated with erythropoietin(100U/kg) injected subcutaneously for 2 weeks. Mean arterial pressure (MAP), cardiac output (CO), cardiac index(CI), splanchnic vascular resistance (SVR), splanchnic blood flow (SBF) and serum NO concentration weredetermined in erythropoietin - treated, erythropoietin - untreated cirrhotic rats and controls by using 57Co - labelledmicrosphere technique and a fluorometric assay, respectively. In addition, blood Hb levels were also measured inthe 3 groups. Results Untreated cirrhotic rats had significantly lower MAP, SVR, Hb and higher Co, CI, SBFand NO concentration than those of the controls (P<0.01). In treated cirrhotic rats, erythropoietin significantlyattenuated the increase of CO, CI, SBF, NO concentration and the decrease of MAP and SVR. In cirrhotic rats,opoetin beta in subcutaneous dose of 100U·kg-1·d-1 induced a markedly increment of blood Hb levels anddecrement of NO concentration in comparison with untreated cirrhotic rats (181±11g/L vs 120±15g/L,1.14±0.62μmol/L vs 4.20±1.25μmol/L). Conclusion The endogenous NO may play an important role in thechanges of hemodynamics pattern in cirrhosis, and hyperdynamic circulatory status in rats with cirrhosis might beameliorated by inactivation of overproduced NO by increasing hemoglobin with erythropoietin.
