Inhibition of RECl3 on Ni2O3-induced DNA breakage of human embryo lung cell (HEL) and reduced content of negative superoxidative ion (O2) in guinea alveolar macrophage (AM ) was observed by means of single cell gel el...Inhibition of RECl3 on Ni2O3-induced DNA breakage of human embryo lung cell (HEL) and reduced content of negative superoxidative ion (O2) in guinea alveolar macrophage (AM ) was observed by means of single cell gel electrophoresis assay (comet assay) and cytochrome C assay respectively. Incubated with 2×10 cell/ml human embryo lung cell for 1 h at 37℃, 20μg. ml-1 of Ni2O3 could obviously induce DNA strand breakage compared with the control (P< 0.01). Add 10 μg.ml-1 CeCl3 or 10μg RECl3 with 20 μg·ml-1 Ni2O3 simultaneously in to HEL culture, the DNA strand breakage caused by Ni2O3 reduction significantly. Culture with 4×10 cell/ ml AM for 1 h at 37℃, 10, 20 μg· ml-1 of Ni2O3 could distinctly increase·O2 content in AM compared with the control (P< 0.05). In the similar way, RECl3, CeCl3 or LaCl3 could evidently decrease·O2 content induced by Ni2O3 in AM (P<0.01), but both kinds of RE compounds can not suppress·O2 content in AM.展开更多
文摘Inhibition of RECl3 on Ni2O3-induced DNA breakage of human embryo lung cell (HEL) and reduced content of negative superoxidative ion (O2) in guinea alveolar macrophage (AM ) was observed by means of single cell gel electrophoresis assay (comet assay) and cytochrome C assay respectively. Incubated with 2×10 cell/ml human embryo lung cell for 1 h at 37℃, 20μg. ml-1 of Ni2O3 could obviously induce DNA strand breakage compared with the control (P< 0.01). Add 10 μg.ml-1 CeCl3 or 10μg RECl3 with 20 μg·ml-1 Ni2O3 simultaneously in to HEL culture, the DNA strand breakage caused by Ni2O3 reduction significantly. Culture with 4×10 cell/ ml AM for 1 h at 37℃, 10, 20 μg· ml-1 of Ni2O3 could distinctly increase·O2 content in AM compared with the control (P< 0.05). In the similar way, RECl3, CeCl3 or LaCl3 could evidently decrease·O2 content induced by Ni2O3 in AM (P<0.01), but both kinds of RE compounds can not suppress·O2 content in AM.
