Oxyresveratrol(ORes,trans-2,4,3′,5′-tetrahydroxy stilbene)naturally exists in mulberry,grapes,peanuts and other plants.It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position compari...Oxyresveratrol(ORes,trans-2,4,3′,5′-tetrahydroxy stilbene)naturally exists in mulberry,grapes,peanuts and other plants.It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol(Res).Hence,ORes has stronger antioxidant activity than resveratrol.In present study,we employed a rat hepatic fibrosis model induced by carbon tetrachloride(CCl_(4))and administrated ORes via gavage feeding to study the protective effects and potential mechanisms of ORes against hepatic fibrosis.We demonstrated that rat liver oxidative damage induced by CCl_(4)was significantly alleviated after ORes feeding.Furthermore,the mRNA transcription levels ofα-smooth muscle actinn(˛-SMA),desmin,and two MMPs(MMP2 and MMP9)were reduced and the expression levels of transforming growth factorβ1(TGF-β1),p-small mother against decapen-taplegic protein(Smad)1/2 and p-extracellular signal-regulated kinases(ERK)1/2 in the liver tissue down-regulated dramatically.In a parallel study with Res,ORes showed more efficacious protective effect than Res against rat liver fibrosis,which is attributed to extended conjugation system due to the extra hydroxyl group at 2-position on ORes making it more electron-rich and susceptible to oxidation than Res.Therefore,dietary consumption of mulberry and other fruits containing ORes may be beneficial in the prevention of liver fibrosis.展开更多
OBJECTIVE Oxyresveratrol(OXY)has many biological activities including anti-inflammation,anti-oxida⁃tive stress,anti-cancer and immunomodulation.However,the mechanisms underlying its effects against hepatocellular carc...OBJECTIVE Oxyresveratrol(OXY)has many biological activities including anti-inflammation,anti-oxida⁃tive stress,anti-cancer and immunomodulation.However,the mechanisms underlying its effects against hepatocellular carcinoma(HCC)remain poorly understood.METHODS Two HCC cell lines,HepG2 and SMMC-7721 cells,were employed.Their proliferation was determined by CCK8 assay.The migration and invasion of cells were examined by wound healing and transwell assay.Metastasis of HCC was detected by in vivo experiment.Meanwhile,transcriptome analysis was applied to explore the mechanisms of OXY.The results of transcriptome analysis were validated by in vitro experiment.Further⁃more,western blot was used to measure the expression of LC3 and p62 protein.RESULTS OXY significantly inhibited the proliferation,migration and invasion of HCC cells in vitro.OXY suppressed the metastasis of HCC in Balb/c mice with attenuated side effects compared to Doxorubicin.Transcription profiling analysis revealed that OXY may affect autophagy related signaling pathway of HepG2 cells.Western blotting showed that OXY significantly inhibite autophagy by downregulating LC3 and upregulating p62 genes expression.CONCLUSION Our study demonstrated that OXY inhibits the metastasis of HCC by inhibiting autophagy,which suggested OXY to be a candidate for HCC metastasis.展开更多
Aim Oxyresveratrol （trans-2,3 ＇ ,4,5 ＇ -tetrahydroxystilbene, OXY） , a natural polyphenolic phyto- chemical presents in mulberry （Morus alba L. ） , has been reported to have various bioactivities. Though OXY has...Aim Oxyresveratrol （trans-2,3 ＇ ,4,5 ＇ -tetrahydroxystilbene, OXY） , a natural polyphenolic phyto- chemical presents in mulberry （Morus alba L. ） , has been reported to have various bioactivities. Though OXY has high structural similarity with resveratrol, which has been identified as a chemopreventive agent, little is known a- bout OXY＇s effect on cancer. The main objective of our study was to investigate the effect of OXY on metastasis in vivo. To establish an experimental metastasis model, male Kunming mice were challenged with H22 cells by tail vein injection, and were given different doses of OXY （20, 40,80 mg · kg^-1 body weight per day） for 14 days in- traperitoneally. Administration of OXY showed a clear anti-metastatic effect. Compared to control group （u - 10） , the numbers of pulmonary nodules and lung weight were significantly decreased in mice of 40 mg · kg^- 1 group （ n = 10, P 〈 0.05） , which results in 54.5% reduction in the number of metastases. Similar inhibitory effects were ob- served both at 20 and 80 mg · kg^-1 groups（n= 10, 34.2% and 35.7% , respectively）. OXY at the doses used caused an increase in spleen index （P 〈 0.05） but not thymus index. Further we observed animal body weights loss and food intake decrease （P 〈 0.05） , suggesting the toxicity of high dose used. Therefore, we suggest that oxyresveratrol may benefit human as a new preventive agent for cancer metastasis.展开更多
桑树(Morus alba L.)根的乙醇浸膏具有抑制蛋白激酶C(PKC)的活性,从中分得的二种二苯乙烯类衍生物:oxyresveratrol和kuwanon Y对PKC的半抑制浓度IC_(50)分别为48μmol·L^(-1)和15μmol·L^(-1)。酶动力学研究显示,oxyresverat...桑树(Morus alba L.)根的乙醇浸膏具有抑制蛋白激酶C(PKC)的活性,从中分得的二种二苯乙烯类衍生物:oxyresveratrol和kuwanon Y对PKC的半抑制浓度IC_(50)分别为48μmol·L^(-1)和15μmol·L^(-1)。酶动力学研究显示,oxyresveratrol对PKC的抑制属于非竞争性抑制。展开更多
Gnetupendin C, a new dimer coupled by a resveratrol and an oxyresveratrol unit, was isolated from the lianas of Gnetum pendulum C.Y. Cheng (Gnetaceae). Its structure was established on the basis of spectroscopic evide...Gnetupendin C, a new dimer coupled by a resveratrol and an oxyresveratrol unit, was isolated from the lianas of Gnetum pendulum C.Y. Cheng (Gnetaceae). Its structure was established on the basis of spectroscopic evidence, especially 2D techniques.展开更多
基金Grant from Hubei Province,China(GRANT number 2019ABA100)。
文摘Oxyresveratrol(ORes,trans-2,4,3′,5′-tetrahydroxy stilbene)naturally exists in mulberry,grapes,peanuts and other plants.It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol(Res).Hence,ORes has stronger antioxidant activity than resveratrol.In present study,we employed a rat hepatic fibrosis model induced by carbon tetrachloride(CCl_(4))and administrated ORes via gavage feeding to study the protective effects and potential mechanisms of ORes against hepatic fibrosis.We demonstrated that rat liver oxidative damage induced by CCl_(4)was significantly alleviated after ORes feeding.Furthermore,the mRNA transcription levels ofα-smooth muscle actinn(˛-SMA),desmin,and two MMPs(MMP2 and MMP9)were reduced and the expression levels of transforming growth factorβ1(TGF-β1),p-small mother against decapen-taplegic protein(Smad)1/2 and p-extracellular signal-regulated kinases(ERK)1/2 in the liver tissue down-regulated dramatically.In a parallel study with Res,ORes showed more efficacious protective effect than Res against rat liver fibrosis,which is attributed to extended conjugation system due to the extra hydroxyl group at 2-position on ORes making it more electron-rich and susceptible to oxidation than Res.Therefore,dietary consumption of mulberry and other fruits containing ORes may be beneficial in the prevention of liver fibrosis.
文摘OBJECTIVE Oxyresveratrol(OXY)has many biological activities including anti-inflammation,anti-oxida⁃tive stress,anti-cancer and immunomodulation.However,the mechanisms underlying its effects against hepatocellular carcinoma(HCC)remain poorly understood.METHODS Two HCC cell lines,HepG2 and SMMC-7721 cells,were employed.Their proliferation was determined by CCK8 assay.The migration and invasion of cells were examined by wound healing and transwell assay.Metastasis of HCC was detected by in vivo experiment.Meanwhile,transcriptome analysis was applied to explore the mechanisms of OXY.The results of transcriptome analysis were validated by in vitro experiment.Further⁃more,western blot was used to measure the expression of LC3 and p62 protein.RESULTS OXY significantly inhibited the proliferation,migration and invasion of HCC cells in vitro.OXY suppressed the metastasis of HCC in Balb/c mice with attenuated side effects compared to Doxorubicin.Transcription profiling analysis revealed that OXY may affect autophagy related signaling pathway of HepG2 cells.Western blotting showed that OXY significantly inhibite autophagy by downregulating LC3 and upregulating p62 genes expression.CONCLUSION Our study demonstrated that OXY inhibits the metastasis of HCC by inhibiting autophagy,which suggested OXY to be a candidate for HCC metastasis.
文摘Aim Oxyresveratrol （trans-2,3 ＇ ,4,5 ＇ -tetrahydroxystilbene, OXY） , a natural polyphenolic phyto- chemical presents in mulberry （Morus alba L. ） , has been reported to have various bioactivities. Though OXY has high structural similarity with resveratrol, which has been identified as a chemopreventive agent, little is known a- bout OXY＇s effect on cancer. The main objective of our study was to investigate the effect of OXY on metastasis in vivo. To establish an experimental metastasis model, male Kunming mice were challenged with H22 cells by tail vein injection, and were given different doses of OXY （20, 40,80 mg · kg^-1 body weight per day） for 14 days in- traperitoneally. Administration of OXY showed a clear anti-metastatic effect. Compared to control group （u - 10） , the numbers of pulmonary nodules and lung weight were significantly decreased in mice of 40 mg · kg^- 1 group （ n = 10, P 〈 0.05） , which results in 54.5% reduction in the number of metastases. Similar inhibitory effects were ob- served both at 20 and 80 mg · kg^-1 groups（n= 10, 34.2% and 35.7% , respectively）. OXY at the doses used caused an increase in spleen index （P 〈 0.05） but not thymus index. Further we observed animal body weights loss and food intake decrease （P 〈 0.05） , suggesting the toxicity of high dose used. Therefore, we suggest that oxyresveratrol may benefit human as a new preventive agent for cancer metastasis.
文摘桑树(Morus alba L.)根的乙醇浸膏具有抑制蛋白激酶C(PKC)的活性,从中分得的二种二苯乙烯类衍生物:oxyresveratrol和kuwanon Y对PKC的半抑制浓度IC_(50)分别为48μmol·L^(-1)和15μmol·L^(-1)。酶动力学研究显示,oxyresveratrol对PKC的抑制属于非竞争性抑制。
文摘Gnetupendin C, a new dimer coupled by a resveratrol and an oxyresveratrol unit, was isolated from the lianas of Gnetum pendulum C.Y. Cheng (Gnetaceae). Its structure was established on the basis of spectroscopic evidence, especially 2D techniques.
