Objectives： To assess the genotoxic effect of a new antitumor ozone-photodynamic therapy using the improvedmodification of the COMET assay. Methods： Xenograft cancer models on 58 rats were used. The sarcoma RA was t...Objectives： To assess the genotoxic effect of a new antitumor ozone-photodynamic therapy using the improvedmodification of the COMET assay. Methods： Xenograft cancer models on 58 rats were used. The sarcoma RA was transplantedsubcutaneously, and after increasing of tumor volume from 0.5 to 4.2 cm3, rats were divided into the four groups： ＂Intact＂--healthy,＂Control＂--with xenografted tumors and no treatment, ＂PDT＂--the rats treated with the photodynamic therapy, ＂PDT ＋ozone＂--the rats were treated with both photodynamic therapy and injections of ozonated saline solution. The toxicity of treatmentwas assessed by DNA damage in leukocytes using the new modification of the COMET assay. The analysis of the ＂COMETs＂ wasperformed following the percentage of DNA in the tail of the ＂COMET＂ （% TDNA）. Results： A combination of PDT and ozonemakes the strongest negative impact on tumor growth. The tumor growth inhibition is associated with low genotoxic exposure ofozone-photodynamic therapy on whole blood leukocytes of cancer rats. Conclusions： A new modification of the COMET assay canprovide the assessment of the genotoxic effect of the antitumor therapy in experimental neoplasia.展开更多
Introduction： Cystitis has high incidence in female population. Inflammation is responsible of irritative symptoms; it issupposed the existence of an arterial obstructive disease connected with bladder ischaemia. Dir...Introduction： Cystitis has high incidence in female population. Inflammation is responsible of irritative symptoms; it issupposed the existence of an arterial obstructive disease connected with bladder ischaemia. Direct bacterial damage and/orinflammation are responsible of the activation of ROS （radicals of oxygen） and consequent cell damage. NF-kB protein is protagonistin ROS synthesis. High levels of 02 in periphereal tissues reduce ischaemia, responsible of recurrent inflammation and increaseantioxidant effects in presence of controlled oxidation. The aim of this study is the essay of anti-inflammatory effects of OOT（oxygen-ozone therapy） in chronic cystitis, employed in combined subministration. OOT properties could decrease bacterialconcentration and eliminate dysuriac disturbances, reducing ischaemic effects. Methods： This study proposes a combined treatment forcystitis with oxygen-ozone AHTM （autohaemotherapy） and intravescical insufflations. It has been selected 8 Women aged 40-55 yearswith recurrent cystitis, associated with pain and urge incontinence. We have used certified medical OOT generator. Results： Aftertherapies, all patients showed sterile urine and progressive reduction of symptoms with a sensible improvement of quality of life.Conclusions： Nevertheless, in a little group of patients, preliminary results could suggest the use of OOT for recurrent cystitis afterfailure of conventional therapies.展开更多
文摘Objectives： To assess the genotoxic effect of a new antitumor ozone-photodynamic therapy using the improvedmodification of the COMET assay. Methods： Xenograft cancer models on 58 rats were used. The sarcoma RA was transplantedsubcutaneously, and after increasing of tumor volume from 0.5 to 4.2 cm3, rats were divided into the four groups： ＂Intact＂--healthy,＂Control＂--with xenografted tumors and no treatment, ＂PDT＂--the rats treated with the photodynamic therapy, ＂PDT ＋ozone＂--the rats were treated with both photodynamic therapy and injections of ozonated saline solution. The toxicity of treatmentwas assessed by DNA damage in leukocytes using the new modification of the COMET assay. The analysis of the ＂COMETs＂ wasperformed following the percentage of DNA in the tail of the ＂COMET＂ （% TDNA）. Results： A combination of PDT and ozonemakes the strongest negative impact on tumor growth. The tumor growth inhibition is associated with low genotoxic exposure ofozone-photodynamic therapy on whole blood leukocytes of cancer rats. Conclusions： A new modification of the COMET assay canprovide the assessment of the genotoxic effect of the antitumor therapy in experimental neoplasia.
文摘Introduction： Cystitis has high incidence in female population. Inflammation is responsible of irritative symptoms; it issupposed the existence of an arterial obstructive disease connected with bladder ischaemia. Direct bacterial damage and/orinflammation are responsible of the activation of ROS （radicals of oxygen） and consequent cell damage. NF-kB protein is protagonistin ROS synthesis. High levels of 02 in periphereal tissues reduce ischaemia, responsible of recurrent inflammation and increaseantioxidant effects in presence of controlled oxidation. The aim of this study is the essay of anti-inflammatory effects of OOT（oxygen-ozone therapy） in chronic cystitis, employed in combined subministration. OOT properties could decrease bacterialconcentration and eliminate dysuriac disturbances, reducing ischaemic effects. Methods： This study proposes a combined treatment forcystitis with oxygen-ozone AHTM （autohaemotherapy） and intravescical insufflations. It has been selected 8 Women aged 40-55 yearswith recurrent cystitis, associated with pain and urge incontinence. We have used certified medical OOT generator. Results： Aftertherapies, all patients showed sterile urine and progressive reduction of symptoms with a sensible improvement of quality of life.Conclusions： Nevertheless, in a little group of patients, preliminary results could suggest the use of OOT for recurrent cystitis afterfailure of conventional therapies.
