目的研究多肽P-15对小牛骨表面生长的大鼠成骨细胞附着、增殖、分化的影响。方法对照组和实验组分别采用单纯无机小牛骨粉(anorganic bone mineral,ABM)和复合P-15的无机小牛骨粉接种大鼠成骨细胞进行体外培养。四甲基偶氮唑蓝[3-(4,5-d...目的研究多肽P-15对小牛骨表面生长的大鼠成骨细胞附着、增殖、分化的影响。方法对照组和实验组分别采用单纯无机小牛骨粉(anorganic bone mineral,ABM)和复合P-15的无机小牛骨粉接种大鼠成骨细胞进行体外培养。四甲基偶氮唑蓝[3-(4,5-dimehyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide,MTT]比色法检测大鼠成骨细胞在2组骨粉表面附着、增殖的速度,倒置相差显微镜观察成骨细胞在2组骨粉表面及周围的增殖情况,用对硝基苯磷酸二钠盐(p-nitrophenyl phosphate,PNPP)法检测2组成骨细胞碱性磷酸酶(alkaline phosphatase,ALP)活性。结果成骨细胞体外培养24h,实验组与对照组MTT比色法检测的光密度值分别为1.597±0.035、1.239±0.063,差异有统计学意义(P<0.01)。培养72h,实验组和对照组PNPP法检测的ALP活性分别为(35.533±1.051)金氏单位/100mL和(34.645±1.187)金氏单位/100mL,实验组略高于对照组,但差异无统计学意义(P>0.05)。结论多肽P-15加快了大鼠成骨细胞在骨粉表面粘附、增殖的速度,但对大鼠成骨细胞的分化无明显促进作用。展开更多
The aim of the present study was to investigate an inorganic bovine-derived hydroxyapatite bone substitute (Osteograph?) mixed with the same biomaterial coated with a synthetic peptide (P-15) analogue of collagen (Pep...The aim of the present study was to investigate an inorganic bovine-derived hydroxyapatite bone substitute (Osteograph?) mixed with the same biomaterial coated with a synthetic peptide (P-15) analogue of collagen (PepGen P-15?). This blend of bone replacement materials was used for sinus floor augmentation. Assessments were carried out by using histology methods, transmission electron microscopy (TEM) and microanalysis (EDX). Ultrastructural and analytical features of the interfaces between the graft material and the peri-biomaterial tissues were evaluated six months after implantation. Our findings clearly show that newly-formed crystallites first develop at the surface of implanted crystals. Histological investigations revealed new bone tissue linking biomaterial particles together. TEM assessments pointed out that lamellar bone was generally separated from the graft material by a layer of woven bone measuring between 1 and 1.5 μm in thickness. Although calcified bone tissue was observed in direct contact with bone filling particles, the presence of mineralized granular material around implanted particles was also noticed. No characteristic periodic striation of mineralized collagen was evident within that mineralized structure. Chemical analyses (TEM-EDX) realized at different locations of newly formed mineralized granular substance along the interface revealed average Ca/P ratios ranging between 1.02 and 1.63. The different, concomitantly occurring, aforementioned structural features of the interfaces strongly suggested that the host responses to the used biomaterial blend resulted from dynamic osseointegration phenomena related to various interfacial mechanisms. Nevertheless, the biological response to the bone graft material appeared clinically and histologically satisfactory.展开更多
Introduction: Lumbar fusion as low back pain treatment continues to be a challenge because of the multiple techniques and materials available, most popular techniques include Transforaminal lumbar interbody fusion (TL...Introduction: Lumbar fusion as low back pain treatment continues to be a challenge because of the multiple techniques and materials available, most popular techniques include Transforaminal lumbar interbody fusion (TLIF), Lateral lumbar interbody fusion (LLIF) and Anterior lumbar interbody fusion (ALIF). Successful lumbar fusion is associated with better clinical outcomes, and it is enhanced and targeted through the use of bone graft materials as an osteogenic cell binding peptide P-15, bound to an anorganic bone mineral (ABM). This peptide improves bone formation when used in fixation devices in a targeted and limited way to the implant surface by activating osteoblast precursor cells;by the osteogenic, osteoinductive and osteoconductive stimuli. The main objective of this study is to standardize the lumbar fusion process in the 3 techniques and achieve a more efficient and predictable lumbar fusion, evaluating results with radiological and clinical scales. Material and Methods: Patients underwent lumbar fusion with the use of P-15 Osteogenic Cell Binding Peptide, bound to an anorganic bone mineral (P-15/ABM) bone graft (5 cc) in three different techniques (TLIF, LLIF, ALIF), achieving a total of 100 lumbar levels. Radiological outcomes included fusion rates per Hounsfield Units at computed tomography (CT) scan and Lenke scale. Clinical outcomes were evaluated via the Oswestry Disability Index (ODI), Short Form Performance (SPF-36) and Visual Analog Scale (VAS and VASs) for pain and satisfaction. Results: 67 patients completed the 12 months follow-up, showing no differences in fusion rates between techniques. (Computed Tomography Hounsfield Units) CTHU reaches more than 200 UH at 3 months follow-up and continues fusion process till 12-month follow-up. Clinical scales showed no disability at ODI, improvement at VAS and VASs scales, absence of health restrictions at SPF-36 score since 6 months follow up. Conclusion: Bone graft volume of 5 cc is adequate for achieving successful lumbar fusion, regardless of the surgical technique employed.展开更多
从Nrf2信号通路探讨淫羊藿苷(icariin,ICA)对D-半乳糖(D-galactose,D-Gal)诱导的小鼠睾丸支持细胞株15P-1细胞损伤的保护作用。将15P-1细胞分为对照组(control)、150 mM D-Gal处理组、D-Gal+ICA(0.5μM)组和D-Gal+ICA(1.0μM)组。RT-PC...从Nrf2信号通路探讨淫羊藿苷(icariin,ICA)对D-半乳糖(D-galactose,D-Gal)诱导的小鼠睾丸支持细胞株15P-1细胞损伤的保护作用。将15P-1细胞分为对照组(control)、150 mM D-Gal处理组、D-Gal+ICA(0.5μM)组和D-Gal+ICA(1.0μM)组。RT-PCR法和Western blot法检测15P-1细胞分泌的相关因子GDNF、BMP4和SCF mRNA和蛋白的表达水平。Western blot法检测15P-1细胞紧密连接相关蛋白Occludin和Claudin-1、Nrf2信号通路相关蛋白Nrf2、HO-1和NQO-1的蛋白表达水平。免疫荧光法检测Nrf2表达及定位。与对照组相比,D-Gal处理组15P-1细胞分泌的相关因子GDNF、BMP4和SCF的mRNA和蛋白表达水平均显著下降,而给予ICA后均显著升高。此外,与对照组相比,D-Gal处理组15P-1细胞Occludin、Claudin-1、Nrf2、HO-1和NQO-1的蛋白表达水平均显著下降,而ICA可显著上调上述相关蛋白的表达。免疫荧光结果进一步显示,ICA可上调15P-1细胞核内Nrf2蛋白表达。ICA可减轻D-Gal诱导的15P-1细胞损伤,并改善其功能,其机制可能与上调Nrf2信号通路相关蛋白有关。展开更多
[Na (N- (p-chlorophenyl ) aza-15--crown--5 ) (Et2O )]2 (Na2W0.5 Mo7.5 O26), Mr= 2119. 4, monoclinic, space group P21/c, a= 17. 803(4), b= 13. 674 (3), c= 14. 610(3), β= 112. 33(3)°, V=3290(1) A3, Z= 2, Dc= 2. 13...[Na (N- (p-chlorophenyl ) aza-15--crown--5 ) (Et2O )]2 (Na2W0.5 Mo7.5 O26), Mr= 2119. 4, monoclinic, space group P21/c, a= 17. 803(4), b= 13. 674 (3), c= 14. 610(3), β= 112. 33(3)°, V=3290(1) A3, Z= 2, Dc= 2. 139 g/cm3, μ= 1.704 mm-1, F (000) = 2079, R = 0. 026, Rw= 0. 030. The supermolecular complexconsists of one [Na2W0.5Mo7.5O28)2- anion in which only two Mo atoms are replaced byW in 25% (M= l/4W+3/4Mo) and the two Na+ are linked symmetrically to neighbouring [W0.5Mo7.5O26]4- units on the right and the left respectively and two complexcanons in which each Na+ deviates from the plane of the four ether oxygens and is coordinated to every hetero--atom in the crownether ring, and is also coordinated to the Oatom of Et2O.展开更多
文摘目的研究多肽P-15对小牛骨表面生长的大鼠成骨细胞附着、增殖、分化的影响。方法对照组和实验组分别采用单纯无机小牛骨粉(anorganic bone mineral,ABM)和复合P-15的无机小牛骨粉接种大鼠成骨细胞进行体外培养。四甲基偶氮唑蓝[3-(4,5-dimehyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide,MTT]比色法检测大鼠成骨细胞在2组骨粉表面附着、增殖的速度,倒置相差显微镜观察成骨细胞在2组骨粉表面及周围的增殖情况,用对硝基苯磷酸二钠盐(p-nitrophenyl phosphate,PNPP)法检测2组成骨细胞碱性磷酸酶(alkaline phosphatase,ALP)活性。结果成骨细胞体外培养24h,实验组与对照组MTT比色法检测的光密度值分别为1.597±0.035、1.239±0.063,差异有统计学意义(P<0.01)。培养72h,实验组和对照组PNPP法检测的ALP活性分别为(35.533±1.051)金氏单位/100mL和(34.645±1.187)金氏单位/100mL,实验组略高于对照组,但差异无统计学意义(P>0.05)。结论多肽P-15加快了大鼠成骨细胞在骨粉表面粘附、增殖的速度,但对大鼠成骨细胞的分化无明显促进作用。
文摘The aim of the present study was to investigate an inorganic bovine-derived hydroxyapatite bone substitute (Osteograph?) mixed with the same biomaterial coated with a synthetic peptide (P-15) analogue of collagen (PepGen P-15?). This blend of bone replacement materials was used for sinus floor augmentation. Assessments were carried out by using histology methods, transmission electron microscopy (TEM) and microanalysis (EDX). Ultrastructural and analytical features of the interfaces between the graft material and the peri-biomaterial tissues were evaluated six months after implantation. Our findings clearly show that newly-formed crystallites first develop at the surface of implanted crystals. Histological investigations revealed new bone tissue linking biomaterial particles together. TEM assessments pointed out that lamellar bone was generally separated from the graft material by a layer of woven bone measuring between 1 and 1.5 μm in thickness. Although calcified bone tissue was observed in direct contact with bone filling particles, the presence of mineralized granular material around implanted particles was also noticed. No characteristic periodic striation of mineralized collagen was evident within that mineralized structure. Chemical analyses (TEM-EDX) realized at different locations of newly formed mineralized granular substance along the interface revealed average Ca/P ratios ranging between 1.02 and 1.63. The different, concomitantly occurring, aforementioned structural features of the interfaces strongly suggested that the host responses to the used biomaterial blend resulted from dynamic osseointegration phenomena related to various interfacial mechanisms. Nevertheless, the biological response to the bone graft material appeared clinically and histologically satisfactory.
文摘Introduction: Lumbar fusion as low back pain treatment continues to be a challenge because of the multiple techniques and materials available, most popular techniques include Transforaminal lumbar interbody fusion (TLIF), Lateral lumbar interbody fusion (LLIF) and Anterior lumbar interbody fusion (ALIF). Successful lumbar fusion is associated with better clinical outcomes, and it is enhanced and targeted through the use of bone graft materials as an osteogenic cell binding peptide P-15, bound to an anorganic bone mineral (ABM). This peptide improves bone formation when used in fixation devices in a targeted and limited way to the implant surface by activating osteoblast precursor cells;by the osteogenic, osteoinductive and osteoconductive stimuli. The main objective of this study is to standardize the lumbar fusion process in the 3 techniques and achieve a more efficient and predictable lumbar fusion, evaluating results with radiological and clinical scales. Material and Methods: Patients underwent lumbar fusion with the use of P-15 Osteogenic Cell Binding Peptide, bound to an anorganic bone mineral (P-15/ABM) bone graft (5 cc) in three different techniques (TLIF, LLIF, ALIF), achieving a total of 100 lumbar levels. Radiological outcomes included fusion rates per Hounsfield Units at computed tomography (CT) scan and Lenke scale. Clinical outcomes were evaluated via the Oswestry Disability Index (ODI), Short Form Performance (SPF-36) and Visual Analog Scale (VAS and VASs) for pain and satisfaction. Results: 67 patients completed the 12 months follow-up, showing no differences in fusion rates between techniques. (Computed Tomography Hounsfield Units) CTHU reaches more than 200 UH at 3 months follow-up and continues fusion process till 12-month follow-up. Clinical scales showed no disability at ODI, improvement at VAS and VASs scales, absence of health restrictions at SPF-36 score since 6 months follow up. Conclusion: Bone graft volume of 5 cc is adequate for achieving successful lumbar fusion, regardless of the surgical technique employed.
文摘[Na (N- (p-chlorophenyl ) aza-15--crown--5 ) (Et2O )]2 (Na2W0.5 Mo7.5 O26), Mr= 2119. 4, monoclinic, space group P21/c, a= 17. 803(4), b= 13. 674 (3), c= 14. 610(3), β= 112. 33(3)°, V=3290(1) A3, Z= 2, Dc= 2. 139 g/cm3, μ= 1.704 mm-1, F (000) = 2079, R = 0. 026, Rw= 0. 030. The supermolecular complexconsists of one [Na2W0.5Mo7.5O28)2- anion in which only two Mo atoms are replaced byW in 25% (M= l/4W+3/4Mo) and the two Na+ are linked symmetrically to neighbouring [W0.5Mo7.5O26]4- units on the right and the left respectively and two complexcanons in which each Na+ deviates from the plane of the four ether oxygens and is coordinated to every hetero--atom in the crownether ring, and is also coordinated to the Oatom of Et2O.
基金supported by the National Natural Science Foundations of China(21476132,51536009,51406109 and 21506118)National High Technology Research and Development Program of China(863 program,2012AA101808)~~
