目的研究异补骨脂素对人皮肤角质形成细胞(Ha Ca T)光老化模型p-ERK1/2表达的影响及对光老化细胞分泌的白介素1α(IL-1α)、肿瘤坏死因子-α(TNF-α)等炎症因子的影响。方法使用照射强度为0.61m W·cm^(-2),照射时间为5min的UVB,建...目的研究异补骨脂素对人皮肤角质形成细胞(Ha Ca T)光老化模型p-ERK1/2表达的影响及对光老化细胞分泌的白介素1α(IL-1α)、肿瘤坏死因子-α(TNF-α)等炎症因子的影响。方法使用照射强度为0.61m W·cm^(-2),照射时间为5min的UVB,建立光老化模型;雌二醇(阳性对照)及异补骨脂素(10^(-7),10^(-6),10-5 mol·L^(-1))处理光老化模型。MTT法检测细胞的活性;GSH、LDH及MDA试剂盒检测雌二醇及不同浓度异补骨脂素对细胞氧化酶活性的影响;Western Blot法检测雌二醇及不同浓度的异补骨脂素对细胞p-ERK1/2、IL-1α及TNF-α蛋白表达量的影响。结果与空白组比较,模型组GSH活性降低,LDH活性升高,MDA含量升高,p-ERK1/2、IL-1α及TNF-α蛋白表达量升高(P<0.01,P<0.05);与模型组比较,雌二醇及不同浓度异补骨脂素可以显著的升高GSH的活性,降低LDH活性,降低MDA含量,降低p-ERK1/2、IL-1α及TNF-α蛋白表达量(P<0.01,P<0.05)。结论异补骨脂素可通过抑制ERK磷酸化,抑制炎症因子的分泌。展开更多
Objective To explore the effect of ＂three-acupoint and five-needling method＂ on the expression level of p-ERK 1/2 protein in lung tissue of rats with allergic bronchial asthma.Methods Forty SPF-grade male SD rats we...Objective To explore the effect of ＂three-acupoint and five-needling method＂ on the expression level of p-ERK 1/2 protein in lung tissue of rats with allergic bronchial asthma.Methods Forty SPF-grade male SD rats were selected and randomly divided into four groups,including control group（group C）,model group（group M）,acupuncture group（group A） and blocker group（group PD）,with 10 rats in each group.After corresponding intervention was given,cell counting was conducted,and the expression level of p-ERK 1/2 protein in the lung tissue of rats was detected via immunohistochemical method and Western blot method.Results The number of leukocyte and eosinophil in the bronchoalveolar lavage fluid of rats in group A and group PD was smaller than that in group M,but larger than that in group C.It was shown based on the immunohistochemical test and Western blot test that the expression level of p-ERK1/2 protein in the lung tissue of rats in group M was higher than that in group C（P0.05）,while the expression level of p-ERK1/2 protein in group A and group PD was lower than that in group M（P0.05）.Conclusion ＂Threeacupoint and five-needling method＂ can effectively relieve the inflammatory response of rats with allergic bronchial asthma,and inhibit the expression level of p-ERK 1/2 protein in the lung tissue of rats with asthma.Acupuncture may alleviate the symptom of allergic bronchial asthma through inhibiting the expression level of p-ERK 1/2 protein.展开更多
Objective To observe the effects of electroacupuncture(EA)at Neiguan(PC6)on protein expressions of proto-oncogene serine/threonine-protein kinase-1(Raf-1),phospho-extracellular signal-regulated kinase 1/2(p-ERK 1/2),a...Objective To observe the effects of electroacupuncture(EA)at Neiguan(PC6)on protein expressions of proto-oncogene serine/threonine-protein kinase-1(Raf-1),phospho-extracellular signal-regulated kinase 1/2(p-ERK 1/2),and extracellular signal-regulated kinase1/2(ERK 1/2)in rats with myocardial hypertrophy.Methods Totally 40 healthy Sprague-Dawley(SD)rats of clean grade were divided into 4 groups according to random digit table,i.e.,the normal group,the展开更多
基金Supported by Project of National Natural Science Foundation of China:81303046Graduate innovation project of North China University of Science and Technology:2016S42
文摘Objective To explore the effect of ＂three-acupoint and five-needling method＂ on the expression level of p-ERK 1/2 protein in lung tissue of rats with allergic bronchial asthma.Methods Forty SPF-grade male SD rats were selected and randomly divided into four groups,including control group（group C）,model group（group M）,acupuncture group（group A） and blocker group（group PD）,with 10 rats in each group.After corresponding intervention was given,cell counting was conducted,and the expression level of p-ERK 1/2 protein in the lung tissue of rats was detected via immunohistochemical method and Western blot method.Results The number of leukocyte and eosinophil in the bronchoalveolar lavage fluid of rats in group A and group PD was smaller than that in group M,but larger than that in group C.It was shown based on the immunohistochemical test and Western blot test that the expression level of p-ERK1/2 protein in the lung tissue of rats in group M was higher than that in group C（P0.05）,while the expression level of p-ERK1/2 protein in group A and group PD was lower than that in group M（P0.05）.Conclusion ＂Threeacupoint and five-needling method＂ can effectively relieve the inflammatory response of rats with allergic bronchial asthma,and inhibit the expression level of p-ERK 1/2 protein in the lung tissue of rats with asthma.Acupuncture may alleviate the symptom of allergic bronchial asthma through inhibiting the expression level of p-ERK 1/2 protein.
文摘Objective To observe the effects of electroacupuncture(EA)at Neiguan(PC6)on protein expressions of proto-oncogene serine/threonine-protein kinase-1(Raf-1),phospho-extracellular signal-regulated kinase 1/2(p-ERK 1/2),and extracellular signal-regulated kinase1/2(ERK 1/2)in rats with myocardial hypertrophy.Methods Totally 40 healthy Sprague-Dawley(SD)rats of clean grade were divided into 4 groups according to random digit table,i.e.,the normal group,the
