The effects of initial concentrations of PNP, doses of TiO2, cations and anions have been investigated to find out the conditions for the maximum degradation of PNP in presence of 254 nm UV light. The rate of photocat...The effects of initial concentrations of PNP, doses of TiO2, cations and anions have been investigated to find out the conditions for the maximum degradation of PNP in presence of 254 nm UV light. The rate of photocatalytic degradation of PNP was increased with increasing TiO2 dose until the dose concentration reached at a value 0.4 g/100 mL. Further increase of TiO2 decreased the degradation. The maximum degradation of PNP was found with the catalyst dose 0.4 g/100 mL at pH 3. The degradation of PNP was decreased with increasing of PNP concentration. About 90% degradation of PNP was observed when 1.0 × 10﹣4 M PNP was irradiated for 2 hours in 0.4 g/100 mL of TiO2 suspension. The effect of Cu(II) and Fe(II) ions on the degradation was also investigated. Addition of Cu(II) ions enhances the percent degradation but excess of Cu(II) ions decreases the degradation. Under the same experimental conditions, the presence of and is found to be detrimental to the photodegradation of PNP. Hydrogen ion concentration of reaction mixture was found to increase continuously during photodegradation suggesting mineralization of PNP.展开更多
Many nitrophenols tend to persist in the environment and they may become public health hazards. Among nitrophenols, p-nitrophenol （PNP） is a priority pollutant that has been widely used as pesticide. PNP is a toxic ...Many nitrophenols tend to persist in the environment and they may become public health hazards. Among nitrophenols, p-nitrophenol （PNP） is a priority pollutant that has been widely used as pesticide. PNP is a toxic compound that enters the environment during manufacturing and processing of a variety of industrial products. This situation generalized its presence in multiple natural ecosystems： rivers, wastewaters, subterranean waters, soil treated by pesticides and urban atmosphere. This study aims to test the ability of wastewater microflora to degrade high concentration of PNP （500 rag/L） aerobically. An identification of the dominant microorganisms involved in the biodegradation is also carried. The cultures are performed using a minimum medium, where PNP is the sole source of carbon, energy and nitrogen. The kinetic of biodegradation is followed for more than 30 days of incubation at 30 ~C on a shaker （150 tours/min）. The obtained results show that more than 90% of PNP initial concentration is decomposed at the end of incubation. The isolation of microorganisms degrading PNP gave two bacterial colonies with different macroscopic aspects. Sequence analysis of 16S ribosomal DNA indicated that the PNP degrading isolates were closely related to members of the species： Pseudomonas aeruginosa and Bacillus cereus.展开更多
文摘The effects of initial concentrations of PNP, doses of TiO2, cations and anions have been investigated to find out the conditions for the maximum degradation of PNP in presence of 254 nm UV light. The rate of photocatalytic degradation of PNP was increased with increasing TiO2 dose until the dose concentration reached at a value 0.4 g/100 mL. Further increase of TiO2 decreased the degradation. The maximum degradation of PNP was found with the catalyst dose 0.4 g/100 mL at pH 3. The degradation of PNP was decreased with increasing of PNP concentration. About 90% degradation of PNP was observed when 1.0 × 10﹣4 M PNP was irradiated for 2 hours in 0.4 g/100 mL of TiO2 suspension. The effect of Cu(II) and Fe(II) ions on the degradation was also investigated. Addition of Cu(II) ions enhances the percent degradation but excess of Cu(II) ions decreases the degradation. Under the same experimental conditions, the presence of and is found to be detrimental to the photodegradation of PNP. Hydrogen ion concentration of reaction mixture was found to increase continuously during photodegradation suggesting mineralization of PNP.
文摘Many nitrophenols tend to persist in the environment and they may become public health hazards. Among nitrophenols, p-nitrophenol （PNP） is a priority pollutant that has been widely used as pesticide. PNP is a toxic compound that enters the environment during manufacturing and processing of a variety of industrial products. This situation generalized its presence in multiple natural ecosystems： rivers, wastewaters, subterranean waters, soil treated by pesticides and urban atmosphere. This study aims to test the ability of wastewater microflora to degrade high concentration of PNP （500 rag/L） aerobically. An identification of the dominant microorganisms involved in the biodegradation is also carried. The cultures are performed using a minimum medium, where PNP is the sole source of carbon, energy and nitrogen. The kinetic of biodegradation is followed for more than 30 days of incubation at 30 ~C on a shaker （150 tours/min）. The obtained results show that more than 90% of PNP initial concentration is decomposed at the end of incubation. The isolation of microorganisms degrading PNP gave two bacterial colonies with different macroscopic aspects. Sequence analysis of 16S ribosomal DNA indicated that the PNP degrading isolates were closely related to members of the species： Pseudomonas aeruginosa and Bacillus cereus.
基金Supported by the National Natural Science Foundation of China under Grant Nos.60375009 69975021 (国家自然科学基金) the National High-Tech Research and Development Plan of China under Grant No.2002AA422230 (国家高技术研究发展计划)
