Backgroud:The electroacupuncture of auricular concha area(EA-ACR)proved to have a good regulatory effect on insomnia and the release of melatonin,which is most closely related to sleep.However,there is little research...Backgroud:The electroacupuncture of auricular concha area(EA-ACR)proved to have a good regulatory effect on insomnia and the release of melatonin,which is most closely related to sleep.However,there is little research to demonstrate the relationship between EA-ACR and melatonin.The objective of present study is to observe the effects of EA-ACR on the circadian rhythm of melatonin secretion in p-chlorophenylalanine(PCPA)-induced insomnia rats.Methods:The study divided 36 male Sprague-Dawley rats randomly into three groups:12 in blank group,12 in model group,and 12 in EA-ACR group.By using PCPA intraperitoneal injection for 2 days,insomnia model was established.EA-ACR group was treated for 28 days.We observed the effects of EA-ACR on the rats'behavioral changes,open-field test and weight during the experiment.After the experiment,the rat tail and heart blood were collected,and the melatonin was detected by enzyme-linked immunosorbent assay.Results:Compared with the blank group,28–30 hours after PCPA intraperitoneal injection,the circadian rhythm of sleep and the total sleep time of model rats decreased.Compared with the model group,electrical stimulation in the ear concha region could effectively reverse the behavioral changes of rats,and increase the trend of weight growth(P=0.010),the crossing score(P=0.014),vertical motion score(P=0.015)and the level of plasma melatonin secretion(P=0.020)with the trend showing a rhythmic pattern(0–60 minutes upward,60–120 minutes downward,120–150 minutes upward).Conclusion:EA-ACR can improve the quality of life(weight),ameliorate exploring irritable and anxiety behaviors in PCPA-induced insomnia rats.The underlying effects of EA-ACR may be excited on the circadian rhythm of melatonin secretion in PCPA-induced insomnia rats.展开更多
目的:观察酸枣仁-五味子药对醇水双提取物对PCPA所致失眠大鼠氨基酸类神经递质的影响,探讨其治疗失眠可能的作用机制。方法:腹腔注射PCPA诱导大鼠失眠模型,造模成功后,灌胃给药,1次/d,连续1 w。HE染色观察大鼠下丘脑病理变化,ELISA法测...目的:观察酸枣仁-五味子药对醇水双提取物对PCPA所致失眠大鼠氨基酸类神经递质的影响,探讨其治疗失眠可能的作用机制。方法:腹腔注射PCPA诱导大鼠失眠模型,造模成功后,灌胃给药,1次/d,连续1 w。HE染色观察大鼠下丘脑病理变化,ELISA法测定下丘脑中GABA、Glu含量,免疫组织化学法测定下丘脑中GABA和Glu蛋白的表达,RT-PCR法检测下丘脑中GABA A Rα1和GABA A Rγ2mRNA的表达。结果:与模型组比较,酸枣仁-五味子药对醇水双提物中、高剂量组不仅能显著提高GABA含量,升高GABA A Rα1和GABA A Rγ2mRNA表达,还能降低Glu水平。结论:酸枣仁-五味子药对具有较好的镇静催眠作用,其作用机制可能与调节下丘脑内氨基酸类神经递质的含量有关。展开更多
基金supported by the Independent Topic Project of China Academy of Chinese Medical Sciences(No.ZZ12007)the National Natural Science Foundation of China(No.81473780).
文摘Backgroud:The electroacupuncture of auricular concha area(EA-ACR)proved to have a good regulatory effect on insomnia and the release of melatonin,which is most closely related to sleep.However,there is little research to demonstrate the relationship between EA-ACR and melatonin.The objective of present study is to observe the effects of EA-ACR on the circadian rhythm of melatonin secretion in p-chlorophenylalanine(PCPA)-induced insomnia rats.Methods:The study divided 36 male Sprague-Dawley rats randomly into three groups:12 in blank group,12 in model group,and 12 in EA-ACR group.By using PCPA intraperitoneal injection for 2 days,insomnia model was established.EA-ACR group was treated for 28 days.We observed the effects of EA-ACR on the rats'behavioral changes,open-field test and weight during the experiment.After the experiment,the rat tail and heart blood were collected,and the melatonin was detected by enzyme-linked immunosorbent assay.Results:Compared with the blank group,28–30 hours after PCPA intraperitoneal injection,the circadian rhythm of sleep and the total sleep time of model rats decreased.Compared with the model group,electrical stimulation in the ear concha region could effectively reverse the behavioral changes of rats,and increase the trend of weight growth(P=0.010),the crossing score(P=0.014),vertical motion score(P=0.015)and the level of plasma melatonin secretion(P=0.020)with the trend showing a rhythmic pattern(0–60 minutes upward,60–120 minutes downward,120–150 minutes upward).Conclusion:EA-ACR can improve the quality of life(weight),ameliorate exploring irritable and anxiety behaviors in PCPA-induced insomnia rats.The underlying effects of EA-ACR may be excited on the circadian rhythm of melatonin secretion in PCPA-induced insomnia rats.
文摘目的:观察酸枣仁-五味子药对醇水双提取物对PCPA所致失眠大鼠氨基酸类神经递质的影响,探讨其治疗失眠可能的作用机制。方法:腹腔注射PCPA诱导大鼠失眠模型,造模成功后,灌胃给药,1次/d,连续1 w。HE染色观察大鼠下丘脑病理变化,ELISA法测定下丘脑中GABA、Glu含量,免疫组织化学法测定下丘脑中GABA和Glu蛋白的表达,RT-PCR法检测下丘脑中GABA A Rα1和GABA A Rγ2mRNA的表达。结果:与模型组比较,酸枣仁-五味子药对醇水双提物中、高剂量组不仅能显著提高GABA含量,升高GABA A Rα1和GABA A Rγ2mRNA表达,还能降低Glu水平。结论:酸枣仁-五味子药对具有较好的镇静催眠作用,其作用机制可能与调节下丘脑内氨基酸类神经递质的含量有关。
