Full grown oocytes derived from Bufo Bufo gargarizans rearing at high temperature environment (24℃),never underwent GVBD after progesterone treatment. No p34cdc2, H1 kinase activity was detected in the oocytes after ...Full grown oocytes derived from Bufo Bufo gargarizans rearing at high temperature environment (24℃),never underwent GVBD after progesterone treatment. No p34cdc2, H1 kinase activity was detected in the oocytes after progesterone stimulation or OA microinjection; Western blotting analysis showed that the level of p3cdc2, and p33 in the oocytes are significantly lower than those in the oocytes derived from the hibernating toads (below 10 ℃). 25S-Met incorporation analysis showed that when the oocytes were incubated at 6℃, synthesis of about thirty defferent polypeptides was promoted or induced, including p34cdc2 and some other p13suc1-binding proteins. All these results indicated that a low temperature environment is essential for the oocytes of Bufo Bufo gargarizans to express and store some cell cycle drivers and its regulators, and to gain the maturation competence. These results will also provide a new clue for explaining the molecular mechanisms why gametogenesis of some organisms depends on a relative low temperature and how to maintain the geographical distribution of some animals.展开更多
Aims: The aim of the study was to assess the correlation between the number of CD34+ , CD117+ , c-met+ , CXCR4+ stem cells mobilized into peripheral blood, left ventricular ejection fraction(LVEF), NT-proBNP levels, a...Aims: The aim of the study was to assess the correlation between the number of CD34+ , CD117+ , c-met+ , CXCR4+ stem cells mobilized into peripheral blood, left ventricular ejection fraction(LVEF), NT-proBNP levels, and myocardial necrosis markers in patients with acute myocardial infarction(AMI). Methods and results: 43 patients with STEMI were enrolled. Stem cells number was measured using flow-cytometer and concentrations of NT-proBNP, SDF-1, G-CSF, VEGF, IL-6, and HGF were measured using ELISA kits. The number of stem cells mobilized early(<12 h) in AMI was significantly, positively correlated with LVEF: r=0.49(P=0.0012) for CD34+ cells, r=0.48(P=0.0018) for CXCR4+ cells, r=0.45(P=0.0043) for CD117+ cells, and r=0.41(P=0.01) for c-met+ cells and negatively correlated with NT-proBNP levels on admission r=- 0.35(P=0.024) for CD34+ cells, r=- 0.42(P=0.007) for CXCR4+ cells. In patients with LVEF ≤ 40% , the peak number of CD34+ , CXCR4+ , CD117+ , and c-met+ stem cells was significantly lower when compared patients with LVEF >40% . The number of CXCR4+ cells on admission and after 24 h was negatively correlated with respective cardiac Troponin I levels(r=- 0.37; P=0.029 and r=- 0.45, P=0.02) and maximum activity of CK-MB(r=- 0.37; P=0.021). No significant correlations between levels of haematopoietic cytokines and LVEF were found. Conclusion: The mobilization of CD34+ , CD117+ , CXCR4+ , c-met+ stem cells into peripheral blood early in STEMI is positively correlated with LVEF and negatively correlated with NT-proBNP levels and myocardial necrosis markers.展开更多
HL-60 cells were synchronized from G1 to S phase boundary with a double thymidine block. Samples of the cells were collected at scheduled time points after the release of the block. It was found with immunoblot analys...HL-60 cells were synchronized from G1 to S phase boundary with a double thymidine block. Samples of the cells were collected at scheduled time points after the release of the block. It was found with immunoblot analysis that the protein expression of cyclin E and D, fluctuated periodically. They both began to increase in G, phase,reached the peak in S phase and declined gradually in G, phase. The protein expression of cyclin-dependent kinase P34cdk2 showed no periodical changes- The antiproliferation effect of retinoic acid or arotinoidethylester on HL-60cells was manifested by a block in Go/G1 of most of the cells and resulted in a marked decrease of the protein expression of cyclin E and D1 and no significant change of P34cdk2. These findings suggest that retinoic acid or arotinoidethylester is able to suppress the proliferation of HL-cells or to induce their differentiation.展开更多
The fluorescent calcium ion indicator dye Fluo-3 and DNA-binding dye Hoechst 33342 were employed to determine, in a quantitative microspectrofluorometric study, the intracellular calcium ion concentration ([Ca^(2+)]_i...The fluorescent calcium ion indicator dye Fluo-3 and DNA-binding dye Hoechst 33342 were employed to determine, in a quantitative microspectrofluorometric study, the intracellular calcium ion concentration ([Ca^(2+)]_i) and the DNA content of individual living NIH3T3 cells. The well-separated excitation and emission properties of these dyes allowed us to establish for each cell both the phase of the cell cycle using DNA content and [Ca^(2+)]_i. We found that the transition from G_1, through S, to the G_2 phase is accompanied by a two-fold increase in [Ca^(2+)]_i. The [Ca^(2+)]_i was inhomologous in each phase of the interphase (G_1, S and G_2) although [Ca^(2+)]_i in the S and G_2 phases was never lower than certain threshold values in the G_1 and S phases respectively. [Ca^(2+)]_i in G_0 cells was lower than that in G_2 cells. These changes in [Ca^(2+)]_i suggest that [Ca^(2+)]_i may be an import regulator of cell cycle progression.展开更多
文摘Full grown oocytes derived from Bufo Bufo gargarizans rearing at high temperature environment (24℃),never underwent GVBD after progesterone treatment. No p34cdc2, H1 kinase activity was detected in the oocytes after progesterone stimulation or OA microinjection; Western blotting analysis showed that the level of p3cdc2, and p33 in the oocytes are significantly lower than those in the oocytes derived from the hibernating toads (below 10 ℃). 25S-Met incorporation analysis showed that when the oocytes were incubated at 6℃, synthesis of about thirty defferent polypeptides was promoted or induced, including p34cdc2 and some other p13suc1-binding proteins. All these results indicated that a low temperature environment is essential for the oocytes of Bufo Bufo gargarizans to express and store some cell cycle drivers and its regulators, and to gain the maturation competence. These results will also provide a new clue for explaining the molecular mechanisms why gametogenesis of some organisms depends on a relative low temperature and how to maintain the geographical distribution of some animals.
文摘Aims: The aim of the study was to assess the correlation between the number of CD34+ , CD117+ , c-met+ , CXCR4+ stem cells mobilized into peripheral blood, left ventricular ejection fraction(LVEF), NT-proBNP levels, and myocardial necrosis markers in patients with acute myocardial infarction(AMI). Methods and results: 43 patients with STEMI were enrolled. Stem cells number was measured using flow-cytometer and concentrations of NT-proBNP, SDF-1, G-CSF, VEGF, IL-6, and HGF were measured using ELISA kits. The number of stem cells mobilized early(<12 h) in AMI was significantly, positively correlated with LVEF: r=0.49(P=0.0012) for CD34+ cells, r=0.48(P=0.0018) for CXCR4+ cells, r=0.45(P=0.0043) for CD117+ cells, and r=0.41(P=0.01) for c-met+ cells and negatively correlated with NT-proBNP levels on admission r=- 0.35(P=0.024) for CD34+ cells, r=- 0.42(P=0.007) for CXCR4+ cells. In patients with LVEF ≤ 40% , the peak number of CD34+ , CXCR4+ , CD117+ , and c-met+ stem cells was significantly lower when compared patients with LVEF >40% . The number of CXCR4+ cells on admission and after 24 h was negatively correlated with respective cardiac Troponin I levels(r=- 0.37; P=0.029 and r=- 0.45, P=0.02) and maximum activity of CK-MB(r=- 0.37; P=0.021). No significant correlations between levels of haematopoietic cytokines and LVEF were found. Conclusion: The mobilization of CD34+ , CD117+ , CXCR4+ , c-met+ stem cells into peripheral blood early in STEMI is positively correlated with LVEF and negatively correlated with NT-proBNP levels and myocardial necrosis markers.
文摘HL-60 cells were synchronized from G1 to S phase boundary with a double thymidine block. Samples of the cells were collected at scheduled time points after the release of the block. It was found with immunoblot analysis that the protein expression of cyclin E and D, fluctuated periodically. They both began to increase in G, phase,reached the peak in S phase and declined gradually in G, phase. The protein expression of cyclin-dependent kinase P34cdk2 showed no periodical changes- The antiproliferation effect of retinoic acid or arotinoidethylester on HL-60cells was manifested by a block in Go/G1 of most of the cells and resulted in a marked decrease of the protein expression of cyclin E and D1 and no significant change of P34cdk2. These findings suggest that retinoic acid or arotinoidethylester is able to suppress the proliferation of HL-cells or to induce their differentiation.
文摘The fluorescent calcium ion indicator dye Fluo-3 and DNA-binding dye Hoechst 33342 were employed to determine, in a quantitative microspectrofluorometric study, the intracellular calcium ion concentration ([Ca^(2+)]_i) and the DNA content of individual living NIH3T3 cells. The well-separated excitation and emission properties of these dyes allowed us to establish for each cell both the phase of the cell cycle using DNA content and [Ca^(2+)]_i. We found that the transition from G_1, through S, to the G_2 phase is accompanied by a two-fold increase in [Ca^(2+)]_i. The [Ca^(2+)]_i was inhomologous in each phase of the interphase (G_1, S and G_2) although [Ca^(2+)]_i in the S and G_2 phases was never lower than certain threshold values in the G_1 and S phases respectively. [Ca^(2+)]_i in G_0 cells was lower than that in G_2 cells. These changes in [Ca^(2+)]_i suggest that [Ca^(2+)]_i may be an import regulator of cell cycle progression.
