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太平洋双色鳗鲡(Anguilla bicolor pacifica)致病性类志贺邻单胞菌的鉴定和中药敏感性研究 被引量:8
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作者 赖晓健 杨方园 李忠琴 《海洋与湖沼》 CAS CSCD 北大核心 2016年第6期1185-1192,共8页
从发病死亡的养殖太平洋双色鳗鲡(Anguilla bicolor pacifica)肝脏中分离到一株优势菌AMSH1,为研究其种属、毒力及中药敏感性,结合BIOLOG微生物鉴定系统和16S r DNA序列分析对该菌株进行鉴定,并通过人工回归感染试验确定其致病性。此外... 从发病死亡的养殖太平洋双色鳗鲡(Anguilla bicolor pacifica)肝脏中分离到一株优势菌AMSH1,为研究其种属、毒力及中药敏感性,结合BIOLOG微生物鉴定系统和16S r DNA序列分析对该菌株进行鉴定,并通过人工回归感染试验确定其致病性。此外,还研究了10味中药单用、10种双联用和4种三联用对该菌株的体外抑菌作用。结果显示该菌株为类志贺邻单胞菌(Plesiomonas shigelloides)。经人工腹腔注射感染健康太平洋双色鳗鲡,证实该菌具有致病性,半致死量LD50为3.2×105CFU/g。药敏试验结果表明,10味中药均有较好的抑菌效果,其中五倍子(Galla chinensis)、丁香(Eugenia caryophllata)和生地榆(Sanguisorba officinalis)的效果最好,7种双联用复方和1种三联用复方均具有协同抑菌作用。本次引起太平洋双色鳗鲡发病的病原为类志贺邻单胞菌,该菌对太平洋双色鳗鲡的致病性及其中药药敏的研究属首次报道。建议选用含五倍子的中药双联用复方或三联用复方进行防治,皆绿色环保。 展开更多
关键词 太平洋双色鳗鲡 类志贺邻单胞菌 病原菌 药敏试验
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盐度对花鳗鲡(Anguilla marmorata)和太平洋双色鳗鲡(A. bicolor pacifica)幼鳗鳃丝及肾脏Na^+/K^+-ATP酶活力的影响 被引量:6
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作者 罗鸣钟 关瑞章 靳恒 《海洋与湖沼》 CAS CSCD 北大核心 2013年第3期807-813,共7页
采用酶学分析的方法,以淡水组为对照,进行了盐度变化对花鳗鲡(Anguilla marmorata)[体质量(10.70±0.92)g]和太平洋双色鳗鲡(A. bicolor pacifica)幼鳗[体质量(12.11±0.79)g]鳃丝及肾脏Na+/K+-ATP酶活力影响的研究。结果表明,... 采用酶学分析的方法,以淡水组为对照,进行了盐度变化对花鳗鲡(Anguilla marmorata)[体质量(10.70±0.92)g]和太平洋双色鳗鲡(A. bicolor pacifica)幼鳗[体质量(12.11±0.79)g]鳃丝及肾脏Na+/K+-ATP酶活力影响的研究。结果表明,经不同的盐度(5、10、18)处理,各处理组的花鳗鲡和太平洋双色鳗鲡幼鳗鳃丝Na+/K+-ATP酶活力均呈现先降低后升高最后再降低并趋于稳定的趋势。前者24h时达到最低值,48h时盐度10处理组上升至与对照组无显著差异(P>0.05),而盐度18处理组显著高于对照组(P<0.05)。后者6h时达到最低值,12h时盐度5和18处理组达到最高值,24h时盐度10处理组达到最高值。盐度变化对花鳗鲡和太平洋双色鳗鲡幼鳗肾脏Na+/K+-ATP酶活力影响不明显。太平洋双色鳗鲡幼鳗鳃丝Na+/K+-ATP酶酶活提升速度及提升幅度均强于花鳗鲡幼鳗,故认为在盐度范围0—18时,太平洋双色鳗鲡幼鳗比花鳗鲡幼鳗对盐度变化具有更强的适应能力。 展开更多
关键词 盐度 花鳗鲡 太平洋双色鳗鲡 幼鳗 Na+/K+-ATP酶
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Heavy metal exposure reduces hatching success of Acartia pacifica resting eggs in the sediment 被引量:8
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作者 JIANG Xiao-dong WANG Gui-zhong +1 位作者 LI Shao-jing HE Jian-feng 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2007年第6期733-737,共5页
The potential effects of three heavy metals (Cu, Pb, and Cd) on hatching success ofAcartia pacifica resting eggs in the sediment of Xiamen Bay were experimentally investigated. The number ofA. pacifica nauplii hatch... The potential effects of three heavy metals (Cu, Pb, and Cd) on hatching success ofAcartia pacifica resting eggs in the sediment of Xiamen Bay were experimentally investigated. The number ofA. pacifica nauplii hatched from the sediment sharply decreased with the increase of metal concentration and exposure time from 3 to 30 d. An increase of the Cu concentration from 34.8 to 348 mg/kg, reduced the number of hatched nauplii by 46.6%-100%. An increase of the Pb concentration from 75.2 to 752 mg/kg, reduced the number of hatched nauplii by 21.4%-78.9%. An increase of the Cd concentration from 0.68 to 6.8 mg/kg, reduced the number of hatched nauplii by 31.6%-94.7%. The number of nauplii also significantly decreased with the increase of mixed-metal concentration and exposure time in the mixed-metal test. Trimmed Spearman-Karber analysis gave sediment metal 72-h LC50 values of 1.25 mmol Cu/kg, 1.73 mmol Pb/kg, and 0.054 mmol Cd/kg, which suggested that Cd was the most toxic to A. pacifica resting eggs in the three tested metals. The results indicate that heavy metals with higher concentrations can reduce recruitment of A. pacifica nauplii from benthic resting eggs to planktonic population. 展开更多
关键词 METAL resting egg hatching success Acartia pacifica
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A potential source of recruitment of Acartia pacifica nauplii: viable benthic resting eggs 被引量:11
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作者 WANGGulzhong JIANGXiaodong LIShaojing WULisheng WUDingxun 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2005年第2期151-158,共8页
Many estuarine and coastal planktonic copepods depend on the hatching of benthic resting eggs for recruitment of nauplii to the water column population. The distribution and abundance of viable resting eggs of Acartia... Many estuarine and coastal planktonic copepods depend on the hatching of benthic resting eggs for recruitment of nauplii to the water column population. The distribution and abundance of viable resting eggs of Acartia pacifica in the Xiamen Bay were determined by the presence of nauplii in the laboratory. The number of viable eggs var/ed temporally and spatially. The maximum number (9.2×10^4m^-2) of viable eggs was in summer. In spring the average abundance of viable eggs was 5.6×10^4 m^-2. The abundances of viable eggs in fall and winter were similar, respectively 2.7×10^4 and 3.3×10^4m^-2, which were the lowest in the year. The numbers of viable eggs inside the stations of the Xiamen Bay were higher than those outside the stations. The viable eggs were found at all depths (0-10 cm),although not in every station. The maximum number did not necessarily occur in the uppermost centimeter of the sediments but often occurred several centimeters below the water-sediment interface. The accumulation of viable eggs in the seabed of a subtropical bay constitutes a potential source of recruitment of nauplii into the pelagic population. 展开更多
关键词 RECRUITMENT resting eggs Acartia pacifica
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Reduction of recruitment of Acartia pacifica nauplii from benthic resting eggs due to organochlorine pesticides 被引量:8
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作者 JIANG Xiao-dong WANG Gui-zhong LI Shao-jing 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2006年第3期552-556,共5页
Many estuarine and coastal planktonic copepods depend on the hatching of benthic resting eggs for recruitment ofnauplii to the water column population. The potential effects of two organochlorine pesticides, hexchlori... Many estuarine and coastal planktonic copepods depend on the hatching of benthic resting eggs for recruitment ofnauplii to the water column population. The potential effects of two organochlorine pesticides, hexchloriobinzene (HCH) and dichlorodiphenyltrichloroethane (DDT), on the recruitment of A ccu'tla pacifica nauplii from benthic resting eggs in the seabed of Xiamen Bay were experimentally investigated. The abundance of A. pacifica nauplii hatched from the sediment significantly decreased with the increase of pesticide concentration. Trimmed Spearman-Karber analysis gave sediment 96-h LC50 values were 84.81 ng/g for HCH, and 157.94 ng/g for DDT. The median AI (Al50) was -0.77, which suggested that the combined effect of riCH and DDT showed a weak effect than individual effects. There was a positive relationship between mortality and exposure time in DDT treatment, while the relationship was not significant in HCH treatment. The results suggest that organochlorine pesticides can reduce recruitment of A. pacifica nauplii from benthic resting eggs to planktonic population. 展开更多
关键词 organochlorine pesticide resting egg RECRUITMENT A cartia pacifica
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Seasonal size composition and abundance distribution of Euphausia pacifica in relation to environmental factors in the southern Yellow Sea
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作者 Tao Zuo Hui Liu 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2019年第2期70-77,共8页
The seasonal size structure and spatial abundance distributions of Euphausia pacifica populations were investigated in the central part of southern Yellow Sea from August 2009 to May 2010.The abundance and biomass of ... The seasonal size structure and spatial abundance distributions of Euphausia pacifica populations were investigated in the central part of southern Yellow Sea from August 2009 to May 2010.The abundance and biomass of E.pacifica were higher in spring and summer,and lower in autumn and winter.The mean abundance and biomass(calculated by carbon)were 74.94 ind./m^3 and 8.23 mg/m^3,respectively.Females with total length(TL)ranging between 10 and 19 mm in summer had a substantial contribution to the population biomass,whereas larvae of TL of 3–7 mm in spring were the main contributor to the population abundance.The sex ratio(female:male)showed a female bias in four seasons.Its value peaked in summer,and then decreased in autumn,spring,and winter successively.Cohort analysis revealed that the length-frequency distribution of E.pacifica could be characterized as one group with large animals(mean TL>12 mm)accompanied by one or two subgroups of small individuals(mean TL<7 mm).Regarding the spatial distribution,juveniles and adults of E.pacifica tend to concentrate in relatively deep water with low temperature(~11℃)and high salinity(>32),whereas its larvae showed more abundance in inshore water with rich chlorophyll a,low salinity(<32),and warm temperature(>11℃),especially in summer and autumn.Associations changed seasonally between stage-specific abundance and environmental factors. 展开更多
关键词 spatial DISTRIBUTION size composition BIOMASS Euphausia pacifica YELLOW Sea
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Population dynamics of Acartia pacifica (Copepoda:Calanoida):the importance of benthic-pelagic coupling
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作者 JIANG Xiaodong WANG Guizhong LI Shaojing 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2006年第6期88-98,共11页
The seasonal occurrence of Acartia pacifica ( Copepoda: Calanoida) and their resting eggs in the sediment of Xiamen Bay were documented between October 2002 and September 2003. The numher of viable eggs in the sedi... The seasonal occurrence of Acartia pacifica ( Copepoda: Calanoida) and their resting eggs in the sediment of Xiamen Bay were documented between October 2002 and September 2003. The numher of viable eggs in the sediment increased from January to May with the increase in the numher of planktonic females. When the population ofA. pacifica disappeared from the water cohinm, the number of eggs in the sediment began to decrease and reached a low value due to lack of input. The peak of nauplii abundance occurred when the hatching potential of eggs from the sediment was high under the natural environment from February to June. The hatching of resting eggs of A. pacifica was essentially temperature-dependent after suspension, while photoperied regimes had no significant effect on the hatching. The mean density of subitaneeus eggs was 1. 122 0 g/cm^3 with a standard deviation (SD) of 0. 000 2 g/cm^3. The mean density of diapause eggs was 1. 151 2 g/cm^3 with a SD of 0.000 1 g/cm^3. The sinking rates of subitaneons eggs ranged from 19.55 to 26.17 m/d, while those of diapause eggs ranged from 30.29 to 31.28 m/d. The comparison of the egg deposition time and egg hatching time suggested that in most cases virtually all subitaneous eggs of A. pacifica would settle to the bottom before their hatching even though the eggs have high potential to hatch. The evidence was provided that the seasonal dynamics of A. pacifica is accompanied by benthic-pelagic coupling. 展开更多
关键词 population dynamics benthic - pelagic coupling Acartia pacifica
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优科豪马Avid轮胎将成为2017款克莱斯勒Pacificas原配胎
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作者 马晓 《轮胎工业》 CAS 2016年第5期301-301,共1页
美国《现代轮胎经销商》(www.moderntiredealer.com)2016年3月22日报道:优科豪马轮胎公司规格为235/65R17 104T的Avid S34RV轮胎(见图1)将成为2017款克莱斯勒Pacificas原配胎,产品将覆盖美国和加拿大市场。"
关键词 AVID pacificas 加拿大市场 系列产品 销售主管
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The Boundary Between Gondwana and Pacifica and the Suturing Ages of Their Allied Terranes in Southwestern China
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作者 Guo Fuxiang Institute of Prediction of Hidden Ore Deposits, Guilin College of Metallurgical Geology, Guilin 541004, Guangxi 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 1991年第1期87-95,共9页
Two terranes formed since the Late Palaeozoic can be distinguished in southwestern China. One is charac-terized by the Permo-Carboniferous ice-rafted marine gravel-bearing clastic formation and the cold-water faunaof ... Two terranes formed since the Late Palaeozoic can be distinguished in southwestern China. One is charac-terized by the Permo-Carboniferous ice-rafted marine gravel-bearing clastic formation and the cold-water faunaof the Gondwana facies, including the Gangmar Co, Lhasa, Sa' gya, Tengchong and Baoshan terranes and theother is marked by the Upper Palaeozoic of the Yangtze type with the Cathaysian flora and the Pacific-typefusulinids, comprising the Changning-Menglian, Shuangjiang-Lancang, Qamdo and Bayan Har terranes. TheLongmu Co-Shuanghu-Dengqen-North Lancang River-Kejie-Mengding suture zone between the two groups ofterranes is the boundary between Gondwana and Pacifica in southwestern China. On the grounds of thesedimentary formation and successive southwestward migration of the Asian nonmarine Jurassic-Cretaceousendemic bivalves, the ages of the suture and some terranes to the southwest of the suture zone are discussed.The Baoshan terrane and the Nyainrong-Sog terrane in the Lhasa composite terrane were firstly pieced togeth-er with the Asian continent in the early Early Jurassic. The northern Tibet-western Yunnan microplate, in-cluding the Gangmar Co, Lhasa and Tengchong terranes, collided with the Asian continent at the end of theEarly Cretaceous Neocomian. 展开更多
关键词 The Boundary Between Gondwana and pacifica and the Suturing Ages of Their Allied Terranes in Southwestern China
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克莱斯勒Pacifica 新车型官图发布
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《中国汽车市场》 2017年第9期6-6,共1页
近日,克莱斯勒官方公布了Pacifica Touring Plus车型的官图。目前北美市场在售的普通燃油版Pacifica包括LX、Touring、Touring-L、Touring-L Plus、限量版共五种配置,搭载3.6L V6自然吸气发动机,这款发动机的最大功率为291马力(214千瓦)... 近日,克莱斯勒官方公布了Pacifica Touring Plus车型的官图。目前北美市场在售的普通燃油版Pacifica包括LX、Touring、Touring-L、Touring-L Plus、限量版共五种配置,搭载3.6L V6自然吸气发动机,这款发动机的最大功率为291马力(214千瓦),峰值扭矩为355牛·米。 展开更多
关键词 新车型 pacifica 克莱斯勒
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Reduction of hydrocarbon contamination on viability of Acartia pacifica benthic resting eggs 被引量:4
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作者 姜晓东 王桂忠 林琼武 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2008年第1期91-96,共6页
The potential effect of hydrocarbon contamination on the hatching success of benthic resting eggs of Acartia pacifica in Xiamen Bay was investigated experimentally. The number of nauplii emerging from the sediment sam... The potential effect of hydrocarbon contamination on the hatching success of benthic resting eggs of Acartia pacifica in Xiamen Bay was investigated experimentally. The number of nauplii emerging from the sediment samples decreased with increasing Fuel Oil #0 concentration. The estimated rate of mortality increased markedly with the increase of Fuel Oil #0 concentration. Successive fuel Oil #0 concentrations from 50 mg/kg to 5000 mg/kg reduced the number of hatched nauplii by 3.8%-100%. The mortality of A. pacifica resting eggs due to Fuel Oil #0 contamination did not significantly increase as time progressed at each concentration level. The LC50 values of resting eggs, changing from 237.12 to 279.59 mg/kg, remained at an almost stable level in two months. The number of A. pacifica nauplii that hatched from the sediment at 10°C was higher than those from the sediment at 30°C, which indicates that the toxicity of Fuel Oil #0 on A. pacifica resting eggs increases with increasing temperature. 展开更多
关键词 深海底休眠卵 烃污染 生存能力 环境污染
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太平洋真宽水蚤(Eurytemora pacifica)Cu/Zn SOD基因克隆及在重金属胁迫下的表达分析 被引量:2
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作者 胡利腾 夏立萍 +2 位作者 武敏敏 陈晋 张建设 《海洋与湖沼》 CAS CSCD 北大核心 2018年第2期384-394,共11页
铜锌超氧化物歧化酶(Superoxide dismutase,SOD)是一种广泛存在于生物体中最主要的抗氧化酶之一,在抵御过多活性氧簇对机体毒害的过程中起重要作用。本研究以太洋真宽水蚤为研究对象,采用RT-PCR与RACE方法克隆得到太平洋真宽水蚤Cu/Zn ... 铜锌超氧化物歧化酶(Superoxide dismutase,SOD)是一种广泛存在于生物体中最主要的抗氧化酶之一,在抵御过多活性氧簇对机体毒害的过程中起重要作用。本研究以太洋真宽水蚤为研究对象,采用RT-PCR与RACE方法克隆得到太平洋真宽水蚤Cu/Zn SOD基因全长c DNA序列。Cu/Zn SOD(Gen Bank登录号:MF289343)基因序列全长837bp,其中完全开放阅读框为456bp编码152个氨基酸,5′非编码区长297bp,3′非编码区长84bp,分子量约为15.050k Da,理论等电点为5.73。序列比较表明,太平洋真宽水蚤Cu/Zn SOD c DNA的氨基酸序列与其他甲壳动物的一致性较高;存在8个蛋白翻译后修饰位点及蛋白家族标签序列,无跨膜结构域与信号肽。在重金属铬与镍胁迫下,通过实时荧光定量PCR对太平洋真宽水蚤Cu/Zn SOD m RNA体内表达特点分析显示,其表达量均呈现先升后降趋势,在暴露12小时后表达量达到峰值;铬胁迫下的表达量略高于镍;联合胁迫下呈现出拮抗作用。本研究结果将有利于深入探讨桡足类Cu/Zn SOD基因的结构与功能,为进一步研究抗氧化分子机理奠定基础。 展开更多
关键词 铜锌超氧化物歧化酶 太平洋真宽水蚤 基因克隆 金属胁迫 实时荧光定量
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太平洋真宽水蚤(Eurytemora pacifica)CRH-BP基因克隆及表达分析
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作者 王斌 武敏敏 +1 位作者 胡利腾 张建设 《海洋与湖沼》 CAS CSCD 北大核心 2019年第1期210-219,共10页
促肾上腺皮质激素释放激素结合蛋白(CRH-BP)是一种糖基化蛋白,与促肾上腺皮质激素释放激素(CRH)具有很高的亲和力,对CRH诱导的脑垂体促肾上腺皮质激素(ACTH)的释放起到抑制作用。本研究采用RT-PCR与RACE基因克隆方法首次获得太平洋真宽... 促肾上腺皮质激素释放激素结合蛋白(CRH-BP)是一种糖基化蛋白,与促肾上腺皮质激素释放激素(CRH)具有很高的亲和力,对CRH诱导的脑垂体促肾上腺皮质激素(ACTH)的释放起到抑制作用。本研究采用RT-PCR与RACE基因克隆方法首次获得太平洋真宽水蚤(Eurytemora pacifica)CRH-BP基因全长cDNA序列(GenBank登录号:MF289345)1950bp,其中ORF区1245bp编码415个氨基酸,5′非编码区841bp,3′非编码区294bp,理论分子量/等电点为45.816/5.87。生物学序列分析结果表明,太平洋真宽水蚤CRH-BP氨基酸序列与桡足类中近亲真宽水蚤同源性最高;具有6个蛋白修饰位点及蛋白家族标签序列,具有明显的跨膜结构域及信号肽。实时荧光定量PCR分析结果表明,太平洋真宽水蚤CRH-BP基因在盐度、温度及pH环境胁迫中的mRNA表达量均具有一定的时间梯度表达性与浓度梯度表达性特征。本文通过对太平洋真宽水蚤CRH-BP基因结构及不同环境因子刺激下的表达特点的研究为探究桡足类在环境应激、生殖生理及免疫调控等方面奠定了理论基础。 展开更多
关键词 太平洋真宽水蚤 促肾上腺激素释放激素结合蛋白 基因克隆 环境胁迫 实时荧光定量PCR
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Triterpenoid Saponins from Roots of Gypsophila pacifica 被引量:1
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作者 NIE Wei,LUO Jian-guang,KONG Ling-yi Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China 《Chinese Herbal Medicines》 CAS 2010年第2期145-147,共3页
Objective To study the chemical constituents from the roots of Gypsophila pacifica.Methods The chemical constituents were isolated by various column chromatographic methods and their structures were identified by spec... Objective To study the chemical constituents from the roots of Gypsophila pacifica.Methods The chemical constituents were isolated by various column chromatographic methods and their structures were identified by spectral data together with physicochemical analysis.Results Five compounds were isolated and identified as 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl gypsogenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(1),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(2),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl gypsogenin 28-O-β-D-glucopyranosyl-(1→3)-[β-D-xylopyranosyl-(1→4)]-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(3),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-glucopyranosyl-(1→3)-[β-D-xylopyranosyl-(1→4)]-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(4),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-α-L-arabino-pyranosyl-(1→4)-α-L-arabinopyranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside(5).Conclusion The five compounds are isolated from this plant for the first time. 展开更多
关键词 CARYOPHYLLACEAE Gypsophila pacifica triterpenoid saponins
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Chrysler PACIFICA
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作者 陈雨 《汽车杂志》 2005年第3期60-60,共1页
美国人造车的概念如同这的电影一样就是要给人气势磅礴的感受,而克莱斯勒产品将美国人这个概念发挥的淋漓尽致,如本文的主角太平洋多功能车,也是把它赋予如同它的名字一样巨大伟岸。
关键词 CHRYSLER pacifica汽车 操控性能 越野功能 技术性能
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铿锵性格:克莱斯勒Pacifica
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作者 正道 《汽车杂志》 2002年第10期32-35,共4页
关键词 克莱斯勒pacifica 运动型旅行车 技术参数 结构配置
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磷虾肌肉营养成分的分析和评价 被引量:16
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作者 王兰 冯晓梅 +3 位作者 吕晴 陈曼华 乔清慧 韩玉谦 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2013年第7期51-55,共5页
为评估太平洋磷虾的营养价值,采用常规营养指标测定方法对太平洋磷虾和南极磷虾肌肉的营养成分进行测定,并对其营养价值进行综合评价和比较。结果表明:太平洋磷虾肌肉(干样)中粗蛋白含量为65.80%,18种氨基酸总量为56.5%,其中8种人体必... 为评估太平洋磷虾的营养价值,采用常规营养指标测定方法对太平洋磷虾和南极磷虾肌肉的营养成分进行测定,并对其营养价值进行综合评价和比较。结果表明:太平洋磷虾肌肉(干样)中粗蛋白含量为65.80%,18种氨基酸总量为56.5%,其中8种人体必需的氨基酸含量为26.01%;南极磷虾肌肉(干样)中粗蛋白含量为62.80%,18种必需氨基酸总量为57.3%,其中8种人体必需氨基酸含量为25.19%。太平洋磷虾肌肉(干样)中粗脂肪含量为8.97%,脂肪酸组成中多不饱和脂肪酸含量达到47.93%,其中EPA和DHA的含量分别达到9.12%和25.82%;南极磷虾肌肉(干样)中粗脂肪含量为5.80%,多不饱和脂肪酸含量达到41.31%,其中EPA和DHA的含量分别达到26.28%和11.42%。太平洋磷虾矿物质和微量元素含量丰富,与南极磷虾含量相当。综合分析认为,太平洋磷虾与南极磷虾均为营养价值较高的虾类,具有良好的开发利用前景。 展开更多
关键词 太平洋磷虾 肌肉 营养成分 蛋白质 氨基酸
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圆背角无齿蚌血细胞培养 被引量:12
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作者 石安静 邱安东 +3 位作者 唐敏 余燕萍 张洪渊 町井昭 《水生生物学报》 CAS CSCD 北大核心 2001年第2期116-122,共7页
用新设计的培养基培养了圆背角无齿蚌的血细胞,在倒置相差显微镜下进行了活体观察及扫描电镜摄影。发现培养的血细胞有无颗粒细胞、颗粒细胞、透明细胞和类淋巴细胞。前三者均能伸出长的伪足和突起,与瓶壁紧密贴附,呈体外培养的成纤... 用新设计的培养基培养了圆背角无齿蚌的血细胞,在倒置相差显微镜下进行了活体观察及扫描电镜摄影。发现培养的血细胞有无颗粒细胞、颗粒细胞、透明细胞和类淋巴细胞。前三者均能伸出长的伪足和突起,与瓶壁紧密贴附,呈体外培养的成纤维细胞型;后者呈圆形,不与瓶壁贴附;四者的比例约为4:2:3:1。在活体内注射或在培养基上加入PHA和ConA,培养2—7d中,每天取部分供加入秋水仙素,用空气干燥法制片,作染色体观察,但未观察到转化细胞和有丝分裂相。研究结果表明,圆背角无齿蚌的颗粒细胞、无颗粒细胞和透明细胞在体外贴附玻璃表面的特征与高等动物的巨噬细胞类似。而不贴瓶的圆形细胞与高等动物的淋巴细胞类似。但在体外培养均不能繁殖,它们可能是高度分化的细胞。 展开更多
关键词 圆背角无齿蚌 血细胞 培养 贝类
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二种微藻多糖与蛋白质提取物的抗菌活性 被引量:27
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作者 陈晓清 郑怡 林雄平 《福建师范大学学报(自然科学版)》 CAS CSCD 北大核心 2005年第2期76-79,共4页
从二种微藻(海水小球藻和紫球藻)中提取粗多糖与粗蛋白质,利用纸片法进行了抑菌实验.结果表明,二种微藻蛋白质与多糖提取物显示了不同程度的抑制细菌和真菌活性.多糖提取物抗菌谱比蛋白质提取物抗菌谱广.海水小球藻多糖提取物对中华根... 从二种微藻(海水小球藻和紫球藻)中提取粗多糖与粗蛋白质,利用纸片法进行了抑菌实验.结果表明,二种微藻蛋白质与多糖提取物显示了不同程度的抑制细菌和真菌活性.多糖提取物抗菌谱比蛋白质提取物抗菌谱广.海水小球藻多糖提取物对中华根霉与稻瘟病菌有极强的抗菌活性.紫球藻多糖提取物抗细菌与抗真菌活性没有明显差别.二种微藻蛋白质提取物抗真菌活性比抗细菌活性大. 展开更多
关键词 海水小球藻 紫球藻 多糖 蛋白质 抗菌活性
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盐度对花鳗鲡和太平洋双色鳗鲡幼鳗生长性能及消化酶活力的影响 被引量:21
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作者 罗鸣钟 关瑞章 靳恒 《水生生物学报》 CAS CSCD 北大核心 2015年第4期653-660,共8页
文章研究了不同盐度对花鳗鲡(Anguilla marmorata)幼鳗和太平洋双色鳗鲡(A.bicolor pacifica)幼鳗生长性能及消化酶活力的影响。将花鳗鲡幼鳗[(9.76±0.36)g]和太平洋双色鳗鲡幼鳗[(11.82±0.04)g]分别在淡水(盐度0‰)与盐度5‰... 文章研究了不同盐度对花鳗鲡(Anguilla marmorata)幼鳗和太平洋双色鳗鲡(A.bicolor pacifica)幼鳗生长性能及消化酶活力的影响。将花鳗鲡幼鳗[(9.76±0.36)g]和太平洋双色鳗鲡幼鳗[(11.82±0.04)g]分别在淡水(盐度0‰)与盐度5‰、10‰、18‰水体中养殖30d,测量每组实验鱼总重后检测胃、肠道和肝脏蛋白酶、淀粉酶和脂肪酶的活力。结果表明,花鳗鲡和太平洋双色鳗鲡在各盐度处理中存活率均为100%,未出现死亡。两种鳗鲡在淡水中生长良好,特定生长率最高,而饵料系数最低。盐度对花鳗鲡幼鳗和太平洋双色鳗鲡幼鳗消化酶活力的影响存在差异,其中花鳗鲡胃、肠道和肝脏蛋白酶活力在各盐度处理中均无显著变化(P>0.05),淀粉酶和脂肪酶活力均随盐度的增加而下降;太平洋双色鳗鲡胃蛋白酶活力在盐度10‰时最大,肝蛋白酶活力在盐度18‰时最大,而淀粉酶和脂肪酶活力在各盐度处理组无显著变化(P>0.05)。这表明盐度对花鳗鲡胃、肠道和肝脏的淀粉酶和脂肪酶活力具有抑制作用,对太平洋双色鳗鲡的蛋白酶活力有一定的激活作用。在相同盐度条件下,不同消化器官中同种消化酶活力存在差异,各盐度的两种鳗鲡肠道中淀粉酶和脂肪酶的活力均显著高于肝脏和胃(P<0.05),胃中蛋白酶活力高于肝脏和肠道,但不显著(P>0.05)。研究发现两种鳗鲡体内脂肪酶活力相对较高,表明其对脂肪具有较强的消化能力。建议在配制花鳗鲡幼鳗和太平洋双色鳗鲡幼鳗饲料时,适当提高粗脂肪比例,有助于促进对营养物质的消化吸收,提高养殖效益。 展开更多
关键词 盐度 花鳗鲡 太平洋双色鳗鲡 生长性能 消化酶
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