A new triterpenoid saponin from the leaves and stems of Panax quinquefolium L.

One new triterpcnoid saponin, quinquenoside L17 （1）, was isolated from the leaves and stems of Panax quinquefolium L., and its structure was elucidated as 20-O-[（β-D-xylopyranosyl-（1-6）-O-β-D-glucopyranosy）]-6-O-β-D-glucopyranosy1-dammar-24-ene- 3,6,12,20-tetraol, by the combination analysis of one-dimensional NMR and two-dimensional NMR, mass spectrometry, CD spectrum and chemical evidences.

Two new acetylated ginsenosides from the roots of Panax quinquefolium

Two new acetylated ginsenosides, 20（S）-protopanaxatriol-6-O-α-L-rhamnopyranosyl （1 → 2）-β-D-6＇-O-acetylglucopyranoside （1） and 20（R）-protopanaxatrol-6-O-α-L-rhamnopyranosyl （1 → 2）-β-o-6＇-O-acetylglucopyranoside （2）, were isolated from the roots of Panax quinquefolium. The structures were elucidated on the basis of spectroscopic techniques and chemical means.

Influence of Panax quinquefolium saponins on increased intracellular Ca^(2+) in PC12 cells

BACKGROUND： Previous studies have demonstrated that intracellular Ca^2＋ （[Ca^2＋]） overload, excitotoxicity, free radical injury, and nitric oxide toxicity are involved in mechanisms of neuronal death in the ischemic brain. OBJECTIVE： To investigate the influence of Panax quinquefo/ium saponins （PQS） on multiple factors-induced Ca^2＋ overload in the rat pheochromocytoma （PC12） cell line. DESIGN, TIME AND SETTING： Intergroup comparison, in vitro study. The experiment was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical University between November 2007 and April 2008. MATERIALS- In vitro cultured PC12 cells in the logarithmic phase were assigned into blank control, model, and drug treatment groups （10 μmol/L nimodipine; 40 μg/L, 100 μg/L, and 250 μg/L PQS）. Nimodipine was purchased from Jiangsu Yangtze River Pharmacy Group Co., China; PQS （purity 〉 95%, HLPC grade） was provided by School of Basic Medical Sciences, Jilin University. Caffeine, Na2S2O4, L-glutamic acid （Glu）, Fura-2/AM, and calcium ionophore A23187 were purchased from Sigma, USA. METHODS： PC12 cells in the model and drug treatment groups were separately incubated in glucose-free Hank＇s buffered saline solution ＋ Na2S2O4 （2 mmol/L） for 6 hours, Glu （200 μmot/L） plus A23187 （0.05 μmol/L） for 6 hours, KCI （50 mmol/L） for 1 hour, and caffeine （5 mmol/L） for 3 hours to establish models of intracellular Ca^2＋ overload induced by oxygen and glucose deprivation, Glu, A23187, high K＋, or caffeine. In addition, control cells were incubated in high-glucose DMEM culture medium. MAIN OUTCOME MEASURES： [Ca^2＋]i changes in PC12 cells exposed to oxygen-glucose deprivation, Glu, A23187, high K^＋, or caffeine were detected using spectrofluorometer. RESULTS： PQS blocked the [Ca^2＋]i increase induced by oxygen-glucose deprivation, Glu, A23187, high K＋, or caffeine. In particular, high-dose PQS was most effective （P 〈 0.01）. PQS significantly inhibited Glu- or caffeine-induced [Ca^2＋]i increases in the absence of extracellular Ca^2＋, but nimodipine did not. CONCLUSION： PQS blocked intracellular Ca^2＋ overload induced by oxygen-glucose deprivation, Glu, A23187, high K^＋, or caffeine. This mechanism might be involved in the attenuation of neuronal apoptosis following ischemic brain injury.

A new ceramide from transgenic crown galls of Panax quinquefolium

A new ceramide （1） was isolated from transgenic crown galls of Panax quinquefolium. The structure was elucidated as （2S, 3S, 4R, 20E）-2-[（2＇R）-2＇-hydroxylpalmitoylamino]-20-hexacosene- 1, 3, 4-triol on the basis of spectroscopic and chemical methods.

Isolation and Characterization of a Bioactive Polysaccharide from Panax Quinquefolium L.

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Research Progress on Antitumor Effect and Mechanism of Panax quinquefolium Linn.

Panax quinquefolium Linn.,belonging to Panax,Araliaceae,contains a variety of active ingredients.In recent years,many studies have shown that P.quinquefolium Linn.has high value and development prospect in inhibiting colon cancer,lung cancer and breast cancer.Recent advances in studies on chemical components,antitumor effects and mechanisms of P.quinquefolium Linn.are reviewed in the paper.

Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

Objective To investigate the effects and underlying mechanisms of Panax quinquefolium saponin(PQS)on energy deficiency in hypoxia-reperfusion(H/R)induced cardiomyocytes.Methods The H/R injury involved hypoxia for 3 h and then reperfusion for 2 h.Cardiomyocytes recruited from neonatal rat ventricular myocytes(NRVMs)were randomly divided into control,H/R,H/R+compound C(C.C),H/R+PQS,and H/R+C.C+PQS groups.BrdU assay,lactase dehydrogenase(LDH)leakage and early apoptosis rate were evaluated to assess cell damages.Contents of high energy phosphate compounds were conducted to detect the energy production.Protein expression levels of adenosine monophosphate-activated protein kinase a(AMPKα),glucose transporter 4(GLUT4),phosphate fructose kinase 2(PFK2),fatty acid translocase/cluster of differentiation 36(FAT/CD36),and acetyl CoA carboxylase 2(ACC2)in the regulatory pathways were measured by Western blotting.Immunofluorescence staining of GLUT4 and FAT/CD36 was used to observe the mobilization of metabolic transporters.Results PQS(50 mg/L)pretreatment significantly alleviated H/R-induced inhibition of NRVMs viability,up-regulation of LDH leakage,acceleration of early apoptosis,and reduction of energy production(P<0.05).Compared with the H/R group,up-regulated expression of AMPKα,GLUT4,PFK2,FAT/CD36 and ACC2 were observed,and more GLUT4 and FAT/CD36 expressions were detected on the membrane in the H/R+PQS group(P<0.05).These effects of PQS on H/R-induced NRVMs were eliminated in the H/R+C.C+PQS group(P<0.05).Conclusion PQS has prominent advantages in protecting NRVMs from H/R-induced cell damages and energy metabolic disorders,by activation of AMPKα-mediated GLUT4-PFK2 and FAT/CD36-ACC2 pathways.

Effects of Panax Quinquefolium Saponin on Phosphatidylinositol 3-Kinase/Serine Threonine Kinase Pathway of Neonatal Rat Myocardial Cells Subjected to Hypoxia

Objective： To explore the effects of Panax Quinquefolium Saponin （PQS） on phosphatidylinositol 3-kinase/serine threonine kinase （P13/Akt） pathway of neonatal rat myocardial cells subjected to hypoxia. Methods： Neonatal rat myocardial cells were cultured in vitro. After the myocardial cell injury was induced by hypoxia, the cells were randomized into 5 groups： the normal group, the model group, the positive control group （Ciclosporin A, 2 p, mol/L）, the low-dose PQS group （PQSL, 25mg/L）, and the high-dose PQS group （PQSH, 50 mg/L）. Morphology and behavior of myocardial cells were observed under an inverted microscope. Apoptosis rate and lactate dehydrogenase （LDH） leakage rate of myocardial cells were determined by colorimetry. Mitochondrial transmembrane potential was assessed using a fluorexon laser. Phospho-glycogen synthase kinase （GSK）-3β and phospho-Akt as well as cytochrome C were determined by Western blot. Results： LDH leakage in the Ciclosporin A group, PQSH group and PQSL group reduced progressively compared with the model group （P〈0.05）. Akt and GSK-3β was strongly phosphorylated after treatment with Ciclosporin A and PQS compared with the model group （P〈0.05, P〈0.01）. Compared with the model group （16.41 ± 1.74; 35.28 ± 6.30）, both the integrated optical density of mitochondrial permeability transition pore （MPTP） and the mitochondrial transmembrane potential significantly increased in the PQSH group （42.74± 2.12; 71.36 ± 6.54） and the PQSL group （39.58± 1.49; 66.99± 5.45; P〈0.05, P〈0.01）. However, the protein of cytochrome C outside the mitochonddon decreased in the PQSH group （273.66 ± 14.61） and the PQSL group （259.62 ± 17.31） compared with the model group （502.41 ± 17.76; P〈0.05）. Conclusion： Through activation of the P13K/Akt pathway and inhibition of the MPTP, PQS might protect the heart against ischemia injury and apoptosis of myocardial cells.

Integrative SMRT sequencing and ginsenoside profiling analysis provide insights into the biosynthesis of ginsenoside in Panax quinquefolium

Panax quinquefolium is one of the most common medicinal plants worldwide.Ginsenosides are the major pharmaceutical components in P.quinquefolium.The biosynthesis of ginsenosides in different tissues of P.quinquefolium remained largely unknown.In the current study,an integrative method of transcriptome and metabolome analysis was used to elucidate the ginsenosides biosynthesis pathways in different tissues of P.quinquefolium.Herein,22 ginsenosides in roots,leaves,and flower buds showed uneven distribution patterns.A comprehensive P.quinquefolium transcriptome was generated through single molecular real-time(SMRT)and second-generation sequencing(NGS)technologies,which revealed the ginsenoside pathway genes and UDP-glycosyltransferases(UGT)family genes explicitly expressed in roots,leaves,and flower buds.The weighted gene co-expression network analysis(WGCNA)of ginsenoside biosynthesis genes,UGT genes and ginsenoside contents indicated that three UGT genes were positively correlated to pseudoginsenoside F11,notoginsenoside R1,notoginsenoside R2 and pseudoginsenoside RT5.These results provide insights into ginsenoside biosynthesis in different tissues ofP.quinquefolium.

Purification and Identification of a Novel Protein Isolated from Panax quinquefolium and Evaluation of its In vitro Antioxidant Properties

Objective:A novel protein was first purified from Panax quinquefolius L.(AGNP),and in vitro antioxidant activities of AGNP were first studied in this work.Methods:AGNP was purified by Ion-exchange chromatography and Gel-filtration chromatography.The chemical characterizations of AGNP were tested by sodium dodecyl sulfate–polyacrylamide gel electrophoresis(SDS-PAGE),high-pressure gel-filtration chromatography,MALDI-TOF-MS and HPLC.In vitro antioxidant effects were tested in simple antioxidant assay including 2,2-diphenylpicrylhydrazyl radical scavenging,superoxide radical(O2^-)scavenging,hydroxyl radical(OH)scavenging,and ferric-reducing ability.Results:The results showed which the content of AGNP measured by Bradford method was 2.42 mg/m L and the subunit molecular weight of AGNP measured by sodium dodecyl sulfate–polyacrylamide gel electrophoresis(SDS-PAGE)was 15 kD.The AGNP molecular weight was 15,114 Da both of SDS-PAGE and mass spectrum purity.The result of high-pressure gel-filtration chromatography demonstrated that the molecular weight of AGNP was 31,086 Da,which implied that AGNP was a homodimer.The in vitro Antioxidant results indicated that AGNP had obvious effects to remove the free radicals in vitro.Conclusion:In conclusion,AGNP had more powerful antioxidant capacity and it can be used as an effective natural antioxidant to alleviate oxidative stress.

Microbial inoculants and garbage fermentation liquid reduced root-knot nematode disease and As uptake in Panax quinquefolium cultivation by modulating rhizosphere microbiota community

Objective:To find a suitable ecological cultivation measure to solve the problem of root-knot nematode disease of Panax quinquefolium(Panacis Quinquefolii Radix)and the heavy metals accumulating in its roots.Methods:Three-year-old P.quinquefolium was treated with four different combinations of microbial inoculant(MI)and garbage fermentation liquid(GFL)[the joint application of‘TuXiu’MI and Fifty potassium MI(TF),the combination use of‘No.1'MI and Fifty potassium MI(NF),‘Gulefeng’poly-γ-glutamic acid MI(PGA),GFL],and the untreated control(CK).Here,high-throughput sequencing,ICP-MS and UPLC were employed to systematically characterize changes of microbial diversity and structure composition,heavy metals(As,Cd and Pb)content and ginsenoside content among different treatments.Results:The results revealed that different MIs and GFL could increase the root dry weight of P.quinquefolium,PGA enhanced it by 83.24%,followed by GFL(49.93%),meanwhile,PGA and GFL were able to lessen root-knot nematode disease incidence by 57.25%and 64.35%.The treatment of PGA and GFL can also effectively reduce heavy metals in roots.The As content in GFL and PGA was decreased by 52.17%and 43.48%respectively,while the Cd and Pb contents of GFL and PGA was decreased somewhat.Additionally,the content of total ginsenosides was increased by 42.14%and 42.07%,in response to TF and NF,respectively.Our metagenomic analysis showed that the relative abundance of particular soil microbial community members related to the biocontrol of root-knot nematode disease and plant pathogen(i.e.,Chaetomium in NF,Xylari in GFL,and Microascus in PGA),heavy metal bioremediation(Hyphomacrobium in PGA and Xylaria in GFL),and nitrogen fixation(Nordella and Nitrospira in TF)was significantly increased;notably,potential harmful microflora,such as Plectosaphaerella and Rhizobacter,were more abundant in the control group.Conclusion:MI and GFL could improve the quality of P.quinquefolium by modifying its rhizosphere microbial community structure and composition,both of them are beneficial to the development of ecological cultivation of P.quinquefolium.

西洋参加工副产物对黄羽肉鸡血清生化指标、抗氧化能力和肉品质的影响

为研究西洋参加工副产物对黄羽肉鸡血清生化指标、抗氧化能力及肉品质的影响。本试验选择7日龄黄羽肉鸡160只,随机分成4组,每组4个重复,Ⅰ组、Ⅱ组、Ⅲ组分别添加1%、3%、5%西洋参加工副产物,Ⅳ组为基础饲粮对照组,试验期100d。结果表明,西洋参加工副产物可显著降低肉鸡血糖含量(P <0.05),并显著降低血清中低密度脂蛋白含量(P <0.05);试验组总抗氧化力和谷胱甘肽过氧化物酶极显著高于对照组(P <0.01);Ⅰ组和Ⅱ组的胸肌、腿肌pH值、亮度、红度和黄度极显著优于对照组(P<0.01);试验组胸肌滴水损失和剪切力显著优于对照组(P <0.05)。在本试验条件下,饲粮中添加1%~3%的西洋参加工副产物,能够较好的调节胸肌、腿肌外观色泽,更符合市场消费的需求。

西洋参根腐病拮抗菌XT-25的生防作用研究

为探讨西洋参根腐病的生物防治技术,本研究以西洋参根腐病病原茄病镰孢菌(Fusarium solani)和土赤壳属的Ilyonectria mors-panacis为靶标,从西洋参根际土中筛选具有拮抗作用的生防菌,通过对峙培养、菌丝抑制活性、抑菌促生物质测定和离体接种防效等试验,初步探讨拮抗菌株对西洋参根腐病的抑菌机制和防治效果。结果表明,分离到1株对2种西洋参致病菌的抑菌率皆在55%以上的菌株XT-25;结合形态学特征及16S rRNA、atpD和rpoB基因序列系统发育分析结果,将菌株XT-25鉴定为淡紫灰链霉菌(Streptomyces lavendulae);菌株XT-25对黄瓜枯萎病菌等6种病原菌的抑菌率为59%~70%。菌株XT-25不仅具有分泌蛋白酶、淀粉酶以及β-1,3葡聚糖酶等抑菌物质的能力,还具有溶解有机磷、无机磷、产氨、固氮、以及分泌IAA和ACC脱氨酶等促生物质能力。菌株XT-25挥发性气体对F.solani的抑菌率为9.04%;其可造成F.solani、I.mors-panacis菌丝的畸形。离体接种西洋参的防效试验结果表明,菌株XT-25对致病菌F.solani和I.mors-panacis引致的西洋参根腐病的防治效果分别为40.00%和24.74%。本研究结果为防治由镰孢菌和土赤壳菌引起的西洋参根腐病提供了生防材料和理论参考。

UPLC法测定西洋参不同部位中皂苷类成分及质量评价研究

本文旨在建立超高效液相色谱-二极管阵列检测器(UPLC-PDA)法同时测定山东西洋参中16种皂苷类成分的方法,并对山东省威海市5个地区11批西洋参芦头、主根、侧根和须根不同部位的皂苷含量进行测定,以期为山东西洋参质量控制与评价提供科学依据。采用Acquity UPLC BEH C_(18)柱(2.1 mm×50 mm,1.7μm),流动相为水-乙腈,梯度洗脱,流速0.4 mL/min,柱温为30℃,进样量为2μL,PDA检测器,检测波长为203 nm。16种皂苷在0.05~1.00 mg/mL范围内线性良好,相关系数均大于0.999 1,加标回收率为92.45%~103.24%,RSD为0.31%~4.60%,灵敏度、准确度及精密度均符合要求。山东省威海地区西洋参芦头、主根、侧根和须根部位皂苷含量分别为(42.69±7.01) mg/g、(32.63±4.71) mg/g、(30.80±5.15) mg/g和(37.55±5.23) mg/g。通过主成分分析及正交偏最小二乘法分析,初步筛选出人参皂苷Re、Rc、Rb_(1)、Rb_(3)和Rb_(2)作为西洋参不同部位鉴别的潜在质量标志物。本研究建立的方法可用于西洋参皂苷含量测定及不同部位的区分,对西洋参质量进行初步评价。

西洋参茎叶皂苷对力竭运动大鼠心肌损伤的改善作用

为了研究西洋参茎叶皂苷减轻力竭运动大鼠心肌损伤的作用及机制,为西洋参茎叶皂苷的综合利用提供参考,本试验分为正常对照组、力竭运动组及西洋参茎叶皂苷低、中、高剂量组(100、200、400 mg/kg),除正常对照组外均需进行力竭游泳训练,4周后检测相关指标变化。结果表明,(1)与力竭运动组相比,西洋参茎叶皂苷中、高剂量组大鼠LVIDs、LVIDd极显著减少(P<0.01),低、中、高剂量组LVEF、LVFS极显著增加(P<0.01),心肌组织cTnⅠ含量及血清CTNK、LDH、ALT、α-HBDH活性均极显著降低(P<0.01);中、高剂量组LVIDs、LVIDd同低剂量组比极显著减少(P<0.01),高剂量组LVEF极显著高于低、中剂量组(P<0.01),中、高剂量组LVFS极显著高于低剂量组(P<0.01),而CTNK、α-HBDH活性极显著低于低剂量组(P<0.01),高剂量组LDH活性极显著低于低、中剂量组(P<0.01),低、中、高剂量组ALT活性比较差异均有极显著性(P<0.01)。(2)与力竭运动组相比,西洋参茎叶皂苷低、中、高剂量组大鼠心肌组织及血清MDA含量极显著降低(P<0.01),除低剂量组大鼠血清GSH-Px活性显著增加(P<0.05)外,其余各组大鼠心肌组织及血清SOD、CAT及GSH-Px活性均极显著增加(P<0.01);高剂量组心肌组织及血清MDA含量极显著低于低、中剂量组(P<0.01),SOD、CAT活性则极显著高于低、中剂量组(P<0.01),中、高剂量组GSH-Px活性极显著高于低剂量组(P<0.01)。(3)与力竭运动组相比,西洋参茎叶皂苷低剂量组大鼠心肌组织细胞凋亡率显著降低(P<0.05),中、高剂量组细胞凋亡率及各剂量组Bax、cleaved caspase-3表达极显著降低(P<0.01),而Bcl-2表达极显著增加(P<0.01);中、高剂量组细胞凋亡率、Bax及cleaved caspase-3表达均极显著少于低剂量组(P<0.01),而Bcl-2表达极显著高于低剂量组(P<0.01)。本研究显示,西洋参茎叶皂苷具有减轻力竭运动大鼠心肌损伤及改善心功能作用,且该作用与抑制氧化应激及抗心肌细胞凋亡有关。

UPLC-MS/MS测定心悦胶囊中6个皂苷类成分的含量

目的:采用超高效液相色谱-质谱法(UPLC-MS/MS)建立心悦胶囊中主要成分人参皂苷Rg_(1)、人参皂苷Re、人参皂苷Rb_(1)、拟人参皂苷F_(11)、人参皂苷Rd和20(S)-人参皂苷F1的含量测定方法。方法:使用Waters ACQUITY BEH C_(18)色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-水为流动相,梯度洗脱,流速为0.5 mL·min^(–1),柱温为40℃,电喷雾电离源,多反应离子监测扫描方式进行检测。结果:6个成分分别在质量浓度为9.638~963.800、9.839~983.900、9.951~995.100、5.270~1054.000、9.608~960.800、4.905~981.000 ng·mL^(–1)时与峰面积线性关系良好(r>0.998),平均加样回收率(RSD)分别为96.9%(2.2%)、100.1%(1.5%)、97.2%(1.8%)、98.5%(2.3%)、96.6%(2.8%)、100.2%(3.5%)。结论:所建立的方法快速、准确、灵敏度高,可用于心悦胶囊中西洋参茎叶总皂苷的质量控制。

基于离子液体原位泡腾萃取法在丹参及西洋参克百威残留分析中的应用

本研究建立了一种新颖的基于酸性离子液体的原位泡腾萃取技术的前处理方法。酸性离子液体1-丁基-3-甲基咪唑硫酸氢盐呈酸性,能与碳酸氢盐反应产生二氧化碳,起到泡腾萃取的效果,它还能与六氟磷酸铵发生离子交换作用而置换成疏水性离子液体1-丁基-3-甲基咪唑六氟磷酸盐,使其与水相分离,通过离心获取萃取相,最后通过高效液相色谱仪测定萃取相中农药的含量。考察了酸性离子液体、碳酸氢钠和氯化钠用量对萃取效率的影响,最终所得最佳条件为200 mg酸性离子液体、0.4 g碳酸氢钠和1.0 g氯化钠。在最优条件下,克百威的线性范围为0.3~4.0μg/mL,日内和日间的相对偏差(RSD)为4.6%和10.2%,丹参和西洋参在4.0μg/mL加标水平下,加标回收率为94.7%(西洋参)和74.6%(丹参),将此方法可用于不同基质中药材和饮片中农药残留的处理。

霍山石斛和西洋参配伍对环磷酰胺诱导的大鼠血虚证模型治疗作用研究

目目的:探讨霍山石斛和西洋参破壁饮片配伍对环磷酰胺诱导的大鼠血虚证模型的治疗作用。方法:大鼠腹腔注射环磷酰胺,建立血虚证模型,制备霍山石斛和西洋参破壁饮片混悬液进行灌胃给药,每天1次,连续给药14d,期间对大鼠的一般生活状态进行观察,并记录体质量;末次给药后对大鼠外周血象指标、免疫和造血相关因子水平进行检测;计算胸腺指数和脾指数。结果:与模型组比较,霍山石斛和西洋参破壁饮片配伍可不同程度提高大鼠体质量,提升外周血中WBC、PLT、RBC、LYM、HGB水平,升高血清中IL-2、IL-6、EPO、M-CSF水平,增加胸腺和脾脏指数。结论:霍山石斛、西洋参配伍对血虚证有一定的治疗作用,具体临床效果需进一步研究。

响应面法优化西洋参果多糖的提取工艺及其体外抗氧化活性

目的:对西洋参果实中的多糖进行提取,结合响应面法对提取工艺进行优化,并对西洋参果多糖是否具有体外抗氧化活性进行研究。方法:本研究以新鲜的西洋参果实为原料,采用了水提醇沉法提取其中的多糖。用单因素实验以及响应面法对提取工艺进行了优化。从DPPH自由基清除率、羟基自由基清除率以及还原能力三个方面进行果多糖的体外抗氧化活性研究。结果:最佳工艺参数为:提取时间为2.5 h,乙醇浓度为80%,料液比为1:16 g/mL,此时的多糖得率为29.47%±0.65%,与模型预测值相当。在以下三方面考察了西洋参果多糖的体外抗氧化活性:多糖浓度为3.4 mg/mL时,其DPPH自由基清除率达75.14%±0.65%,IC_(50)值为0.71 mg/mL;多糖浓度为3.4 mg/mL时,其羟基自由基的清除率可达71.82%±1.43%,IC_(50)值为0.87 mg/mL;多糖的浓度为1.0 mg/mL时,其总还原力达到了0.730,并且其体外抗氧化能力随西洋参果多糖浓度的增加而增强。结论:抗氧化活性的实验结果说明了西洋参果多糖具有较好的抗氧化活性。本研究可以为西洋参果多糖进一步开发利用奠定理论基础,确保中药资源能够被充分利用。

大别山区林地环境对林下西洋参生长的影响

通过比较大别山区不同海拔和林分类型的林下栽培西洋参地上和地下部位生长量的差异,分析大别山区林地环境对西洋参生长的影响。结果表明:海拔、林分类型和落叶层厚度均对林下西洋参的生长有显著影响,海拔1250~1300 m的针阔混交林的林地环境更适合高山林下西洋参生长,一定厚度的落叶层有助于西洋参根部的生长。