期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
PA1b, a plant peptide, induces intracellular [Ca^(2+)] in- crease via Ca^(2+) influx through the L-type Ca^(2+) channel and triggers secretion in pancreatic β cells 被引量:5
1
作者 HU ZhiTao1, DUN XinPeng1, ZHANG Ming1, ZHU HongLiang1, XIE Li1, WU ZhengXing1, CHEN ZhengWang1 & XU Tao1,2 1 Joint Laboratory of Institute of Biophysics and Huazhong University of Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China 2 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. 《Science China(Life Sciences)》 SCIE CAS 2007年第3期285-291,共7页
Using alginic acid to adsorb polypeptides at pH 2.7, we isolated a peptide pea albumin 1b (PA1b) from pea seeds. The PA1b is a single chain peptide consisting of 37 amino acid residues with 6 cysteines which constitut... Using alginic acid to adsorb polypeptides at pH 2.7, we isolated a peptide pea albumin 1b (PA1b) from pea seeds. The PA1b is a single chain peptide consisting of 37 amino acid residues with 6 cysteines which constitutes the cystine-knot structure. Using microfluorometry and patch clamp techniques, we found that PA1b significantly elevated the intracellular calcium level ([Ca2+ ]i) and elicited membrane capacitance increase in the primary rat pancreatic β cells. The PA1b effect on [Ca2+]i elevation was abolished in the absence of extracellular Ca2+ or in the presence of L-type Ca2+ channel blocker, ni- modipine. Interestingly, we found that PA1b significantly depolarized membrane potential, which could lead to the opening of voltage-dependent L-type Ca2+ channels and influx of extracellular Ca2+, and then evoke robust secretion. In this study we identified the plant peptide PA1b which is capable of affecting the excitability and function of mammalian pancreatic β cell. 展开更多
关键词 channel and triggers secretion in pancreatic PA1b influx through the L-type Ca a plant peptide type
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部