Different concentrations of jasmonic acid(JA)and benzothiadiazole(BTH) were sprayed on 2-year-old Rosa rugosa‘Plena’ seedlings. The induced resistance of JA and BTH to Sphaerotheca pannosa(Wallr.) and the changes of...Different concentrations of jasmonic acid(JA)and benzothiadiazole(BTH) were sprayed on 2-year-old Rosa rugosa‘Plena’ seedlings. The induced resistance of JA and BTH to Sphaerotheca pannosa(Wallr.) and the changes of their related physiological indices were investigated. Results showed that JA and BTH treatments had inhibitory impacts on S. pannosa infection. The optimal concentration of JA and BTH was 0.5 mmol/L for the disease-resistance induction of the leaves, its inductive effect was up to 66.36% for BTH and 54.49% for JA. Our results confirmed that exogenous JA and BTH significantly improved R. rugose ‘Plena’ resistance to S. pannosa. When treated with JA and BTH, activities of the three defense enzymes(POD, PPO, and PAL) increased significantly.Contents of total phenolics, flavonoids, and lignin also increased significantly. It is inferred from these results that exogenous JA and BTH could improve the resistance of R.rugose ‘Plena’ to S. pannosa through enhancing activities of the defensive enzymes and accumulation of secondary metabolites in the leaves.展开更多
The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3...The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3.8%, 35.2% and 24.3%, respectively. 3,6-Anhydro-α-D-galactose, β-D-galactose, α-D-galactose and D-glucose were identified by liquid and thin-layer chromatography. Fourier transform infrared (FTIR) spectra of the polysaccharide resembled that of ι-carrageenan. From the <sup>1</sup>H- and <sup>13</sup>C-NMR spectra, 1,3-linked β-D-galactose, 1,4-linked anhydro-α-D-galactose, 1,4-linked α-D-galactose, 1,4-linked β-D-glucose and pyruvic acid (carboxyl acetal, methyl proton and methyl carbon) were assigned. Methylation analysis revealed terminal D-galactose 0.1 mol), 1,4-linked D-glucose (1.0 mol) and 1,2,3,4,6-linked D-galactose (3.7 mol) for native polysaccharide, and terminal D-galactose, 1,4-linked D-galactose (1.9 mol), 1,4-linked D-glucose (1.0 mol), 1,3- linked D-galactose (1.7 mol), and 1,3,4,6-linked D-galactose (0.3 mol) which substituted with pyruvate group at 4 and 6 positions for desulfated polysaccharide. The polysaccharide was the novel pyruvated glucogalactan sulfate, the structure of which was proposed.展开更多
The researches through Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) indicated that Sphaerotheca pannosa directly intrude in the place of concave of rose leaves,form filiform haustorium...The researches through Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) indicated that Sphaerotheca pannosa directly intrude in the place of concave of rose leaves,form filiform haustorium in the epidermal cells,absorb the nutrient and water,and destory the internal structure of mitochondrions,etc.when epidermal cells are filled with filiform haustorium.展开更多
研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比...研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比，EC90值无显著差异；经500～1000mg／L的纳他霉素处理可显著抑制冬枣丙二醛（MDA）含量的增加，并使多聚半乳糖醛酸酶（PG）与苯丙氨酸解氨酶（PAL）的活性提高，对保持果实硬度有很好的效果，并且抑制了可溶性固形物、抗坏血酸（Vc）含量的下降。经1000mg／L纳他霉素处理的冬枣贮藏到60d其商品率迭87．3％，高于对照组的商品率（72．9％）；贮藏到100d其商品率仍可达到75．0％，显著高于对照组（50．2％）。展开更多
基金supported by the Science Foundation of Heilongjiang Province of China(No.QC2014C012)the Fundamental Research Funds for the Central Universities(NO.2572016CA11)
文摘Different concentrations of jasmonic acid(JA)and benzothiadiazole(BTH) were sprayed on 2-year-old Rosa rugosa‘Plena’ seedlings. The induced resistance of JA and BTH to Sphaerotheca pannosa(Wallr.) and the changes of their related physiological indices were investigated. Results showed that JA and BTH treatments had inhibitory impacts on S. pannosa infection. The optimal concentration of JA and BTH was 0.5 mmol/L for the disease-resistance induction of the leaves, its inductive effect was up to 66.36% for BTH and 54.49% for JA. Our results confirmed that exogenous JA and BTH significantly improved R. rugose ‘Plena’ resistance to S. pannosa. When treated with JA and BTH, activities of the three defense enzymes(POD, PPO, and PAL) increased significantly.Contents of total phenolics, flavonoids, and lignin also increased significantly. It is inferred from these results that exogenous JA and BTH could improve the resistance of R.rugose ‘Plena’ to S. pannosa through enhancing activities of the defensive enzymes and accumulation of secondary metabolites in the leaves.
文摘The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3.8%, 35.2% and 24.3%, respectively. 3,6-Anhydro-α-D-galactose, β-D-galactose, α-D-galactose and D-glucose were identified by liquid and thin-layer chromatography. Fourier transform infrared (FTIR) spectra of the polysaccharide resembled that of ι-carrageenan. From the <sup>1</sup>H- and <sup>13</sup>C-NMR spectra, 1,3-linked β-D-galactose, 1,4-linked anhydro-α-D-galactose, 1,4-linked α-D-galactose, 1,4-linked β-D-glucose and pyruvic acid (carboxyl acetal, methyl proton and methyl carbon) were assigned. Methylation analysis revealed terminal D-galactose 0.1 mol), 1,4-linked D-glucose (1.0 mol) and 1,2,3,4,6-linked D-galactose (3.7 mol) for native polysaccharide, and terminal D-galactose, 1,4-linked D-galactose (1.9 mol), 1,4-linked D-glucose (1.0 mol), 1,3- linked D-galactose (1.7 mol), and 1,3,4,6-linked D-galactose (0.3 mol) which substituted with pyruvate group at 4 and 6 positions for desulfated polysaccharide. The polysaccharide was the novel pyruvated glucogalactan sulfate, the structure of which was proposed.
文摘The researches through Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) indicated that Sphaerotheca pannosa directly intrude in the place of concave of rose leaves,form filiform haustorium in the epidermal cells,absorb the nutrient and water,and destory the internal structure of mitochondrions,etc.when epidermal cells are filled with filiform haustorium.
文摘研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比，EC90值无显著差异；经500～1000mg／L的纳他霉素处理可显著抑制冬枣丙二醛（MDA）含量的增加，并使多聚半乳糖醛酸酶（PG）与苯丙氨酸解氨酶（PAL）的活性提高，对保持果实硬度有很好的效果，并且抑制了可溶性固形物、抗坏血酸（Vc）含量的下降。经1000mg／L纳他霉素处理的冬枣贮藏到60d其商品率迭87．3％，高于对照组的商品率（72．9％）；贮藏到100d其商品率仍可达到75．0％，显著高于对照组（50．2％）。
