Simple saccharides have a variety of biological functions,but their structural diversity and inherent structural features pose a major challenge for rapid analysis.In this work,we developed a derivative-free and ion m...Simple saccharides have a variety of biological functions,but their structural diversity and inherent structural features pose a major challenge for rapid analysis.In this work,we developed a derivative-free and ion mobility-free method for the rapid analysis of monosaccharides and disaccharides using paper spray tandem mass spectrometry.Trimeric cluster ions consisting of saccharide analytes,ligands and transition metal ions are used as precursor ions.We defined the R-value as the ratio of the intensity of the product ion that loses one molecule of ligand over the intensity of the product ion that loses one molecule of saccharide via collision induced dissociation(CID).The species and conformation of simple saccharides can be easily differentiated by calculating this R-value.With the capability of directly analyzing clinical samples using paper spray ionization,our method can be used to rapidly quantify the molar ratio of galactose to glucose in dried plasma samples to aid in the diagnosis of galactosemia.The analytical strategy provided herein has good potential to be applied to a wide range of saccharide analysis applications in the future.展开更多
基金supported by NNSF China(No.82072247)Research Projects of Beijing University of Chinese Medicine(Nos.2021-JYB-XJSJJ-001,XJYS21005 and 2021-SYJS-007)。
文摘Simple saccharides have a variety of biological functions,but their structural diversity and inherent structural features pose a major challenge for rapid analysis.In this work,we developed a derivative-free and ion mobility-free method for the rapid analysis of monosaccharides and disaccharides using paper spray tandem mass spectrometry.Trimeric cluster ions consisting of saccharide analytes,ligands and transition metal ions are used as precursor ions.We defined the R-value as the ratio of the intensity of the product ion that loses one molecule of ligand over the intensity of the product ion that loses one molecule of saccharide via collision induced dissociation(CID).The species and conformation of simple saccharides can be easily differentiated by calculating this R-value.With the capability of directly analyzing clinical samples using paper spray ionization,our method can be used to rapidly quantify the molar ratio of galactose to glucose in dried plasma samples to aid in the diagnosis of galactosemia.The analytical strategy provided herein has good potential to be applied to a wide range of saccharide analysis applications in the future.
