The effects of tanshinone II A on cell signal transduction system protein kinase B in rats with myocardial hypertrophy induced by the abdominal aorta partial coarctation were investigated.Rat models of myocardial hype...The effects of tanshinone II A on cell signal transduction system protein kinase B in rats with myocardial hypertrophy induced by the abdominal aorta partial coarctation were investigated.Rat models of myocardial hypertrophy were established by using abdom-inal aorta partial coarctation method.Forty-eight rats were randomly divided into sham group(S group),model group(M group),valsartan treatment group(X group),low-dose tanshinone treatment group(LD group),medium-dose tanshinone treatment group(MD group),and high-dose tanshinone treatment group(HD group)(n=8 in each group).Left ventricular mass index(LVMI),left ventri-cular posterior wall(LVPW),and septal thickness(IVS)were detected by high frequency ultrasonography.Myocardialfiber diameter(MFD)was examined by Hematoxylin-Eosin(HE)staining,and the contents of phosphorylated protein kinase B(p-Akt)and p-Gsk3βin myocardium were assayed by Western blot.The results showed that compared with S group,the values of LVMI,LVPW,IVS and MFD were increased in other groups(P<0.05),and the contents of p-Akt,and p-Gsk3βwere also increased in other groups.As compared with MD group,the values of LVMI,LVPW,IVS and MFD were decreased in all treatment groups(P<0.05),and the contents of p-Akt,and p-Gsk3βwere also decreased in all treatment groups.However,there were no significant differences among LD,MD,and HD groups(P>0.05),and there were no significant differences between X group and tanshinone treatment groups(P>0.05).It was suggested that tanshinone II A could prevent myocardial hypertrophy by its action on the Akt signaling pathway.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.30500657).
文摘The effects of tanshinone II A on cell signal transduction system protein kinase B in rats with myocardial hypertrophy induced by the abdominal aorta partial coarctation were investigated.Rat models of myocardial hypertrophy were established by using abdom-inal aorta partial coarctation method.Forty-eight rats were randomly divided into sham group(S group),model group(M group),valsartan treatment group(X group),low-dose tanshinone treatment group(LD group),medium-dose tanshinone treatment group(MD group),and high-dose tanshinone treatment group(HD group)(n=8 in each group).Left ventricular mass index(LVMI),left ventri-cular posterior wall(LVPW),and septal thickness(IVS)were detected by high frequency ultrasonography.Myocardialfiber diameter(MFD)was examined by Hematoxylin-Eosin(HE)staining,and the contents of phosphorylated protein kinase B(p-Akt)and p-Gsk3βin myocardium were assayed by Western blot.The results showed that compared with S group,the values of LVMI,LVPW,IVS and MFD were increased in other groups(P<0.05),and the contents of p-Akt,and p-Gsk3βwere also increased in other groups.As compared with MD group,the values of LVMI,LVPW,IVS and MFD were decreased in all treatment groups(P<0.05),and the contents of p-Akt,and p-Gsk3βwere also decreased in all treatment groups.However,there were no significant differences among LD,MD,and HD groups(P>0.05),and there were no significant differences between X group and tanshinone treatment groups(P>0.05).It was suggested that tanshinone II A could prevent myocardial hypertrophy by its action on the Akt signaling pathway.
