[ Objective ] The paper was to isolate and preliminarily identify the antibacterial active substances of antagonistic actinomyeete strain G19 obtained from the soil highly affected by peach crown gall （Agrobacterium ...[ Objective ] The paper was to isolate and preliminarily identify the antibacterial active substances of antagonistic actinomyeete strain G19 obtained from the soil highly affected by peach crown gall （Agrobacterium tumefaciens）. [ Method] The antibacterial substances of antagonistic actinomycete strain G19 were ex- tracted using protein precipitation method, then isolated and purified using high performance liquid chromatography and medium-pressure preparative chromatogra- phy. Its molecular weight was determined by MALDI-TOFMS method, and the related functional groups were verified through chemical color reaction. [ Result] Seven peptide portions were produced from the antibacterial substances of antagonistic actinomycete strain G19 with the molecular weights of 900 - 1 300 Da after isolation and purification. It could be also inferred that it contained Cys, and carried with H2O and Na＋. Color reaction of functional groups verified that the sub- stance was polypeptide containing glycosyl. [ Conclusion] The result provided basis for the final definition of the structure of antibacterial substances in antagonistic actinomycete strain G19.展开更多
【目的】筛选拮抗根癌病的生防细菌,为根癌病的生物防治提供材料。【方法】采用平板拮抗法测定桃树根际、茎和土壤中细菌对根癌土壤杆菌Agrobacterium tumefaciens的拮抗活性;以向日葵和番茄为感病植物,测定拮抗菌的防病效果;通过形态...【目的】筛选拮抗根癌病的生防细菌,为根癌病的生物防治提供材料。【方法】采用平板拮抗法测定桃树根际、茎和土壤中细菌对根癌土壤杆菌Agrobacterium tumefaciens的拮抗活性;以向日葵和番茄为感病植物,测定拮抗菌的防病效果;通过形态特征、生理生化特性和16S r DNA序列分析,明确高效生防菌的分类地位。【结果】利用平板拮抗法从125株桃根际细菌中,筛选出2株可在YEB培养基平板上产生抑菌圈的拮抗菌株51-A和51-B和5株没有抑菌圈但长势良好的菌株。将浓度为106cfu·m L-1的待测菌的菌悬液和A.tumefaciens菌悬液以10∶1的比例混和接种向日葵7 d后,菌株51-A和51-B可显著抑制根癌瘤的形成,防效分别为98.0%和97.0%;提前接种拮抗菌菌悬液,第2天接种病原菌菌悬液,可明显抑制番茄根癌瘤的形成,防效分别为91.9%和86.5%。通过形态特征、生理生化特性和16S r DNA序列分析,将51-A和51-B归为粪产碱菌A.faecalis。【结论】粪产碱菌A.faecalis 51-A和51-B对A.tumefaciens引起的根癌病具有强烈抑制作用,可用于根癌病的防治。展开更多
基金Supported by Beijing Natural Science Foundation( 5112010)Beijing Municipal Education Commission Grant ( KM200910020001)~~
文摘[ Objective ] The paper was to isolate and preliminarily identify the antibacterial active substances of antagonistic actinomyeete strain G19 obtained from the soil highly affected by peach crown gall （Agrobacterium tumefaciens）. [ Method] The antibacterial substances of antagonistic actinomycete strain G19 were ex- tracted using protein precipitation method, then isolated and purified using high performance liquid chromatography and medium-pressure preparative chromatogra- phy. Its molecular weight was determined by MALDI-TOFMS method, and the related functional groups were verified through chemical color reaction. [ Result] Seven peptide portions were produced from the antibacterial substances of antagonistic actinomycete strain G19 with the molecular weights of 900 - 1 300 Da after isolation and purification. It could be also inferred that it contained Cys, and carried with H2O and Na＋. Color reaction of functional groups verified that the sub- stance was polypeptide containing glycosyl. [ Conclusion] The result provided basis for the final definition of the structure of antibacterial substances in antagonistic actinomycete strain G19.
文摘【目的】筛选拮抗根癌病的生防细菌,为根癌病的生物防治提供材料。【方法】采用平板拮抗法测定桃树根际、茎和土壤中细菌对根癌土壤杆菌Agrobacterium tumefaciens的拮抗活性;以向日葵和番茄为感病植物,测定拮抗菌的防病效果;通过形态特征、生理生化特性和16S r DNA序列分析,明确高效生防菌的分类地位。【结果】利用平板拮抗法从125株桃根际细菌中,筛选出2株可在YEB培养基平板上产生抑菌圈的拮抗菌株51-A和51-B和5株没有抑菌圈但长势良好的菌株。将浓度为106cfu·m L-1的待测菌的菌悬液和A.tumefaciens菌悬液以10∶1的比例混和接种向日葵7 d后,菌株51-A和51-B可显著抑制根癌瘤的形成,防效分别为98.0%和97.0%;提前接种拮抗菌菌悬液,第2天接种病原菌菌悬液,可明显抑制番茄根癌瘤的形成,防效分别为91.9%和86.5%。通过形态特征、生理生化特性和16S r DNA序列分析,将51-A和51-B归为粪产碱菌A.faecalis。【结论】粪产碱菌A.faecalis 51-A和51-B对A.tumefaciens引起的根癌病具有强烈抑制作用,可用于根癌病的防治。
