In order to explore the forestation technique of pecan in Yunan, different types of pecan seedlings, bare-rooted seedlings and container seedlings were plant- ed in Yunnan, and their survival rates were investigated. ...In order to explore the forestation technique of pecan in Yunan, different types of pecan seedlings, bare-rooted seedlings and container seedlings were plant- ed in Yunnan, and their survival rates were investigated. This study will provide im- portant guidance for development of pecan industry in Yunnan Province.展开更多
Euplatypus segnis is an insect pest of economic importance in pecan (Carya illinoensis) trees grown at Parras, General Cepeda and Torreón Coahuila, Mexico. The objectives in this study-were to identify the fungal...Euplatypus segnis is an insect pest of economic importance in pecan (Carya illinoensis) trees grown at Parras, General Cepeda and Torreón Coahuila, Mexico. The objectives in this study-were to identify the fungal strains associated to ambrosia borer body and diseased pecan wood and determine their pathogenicity. The results showed that the associated fungi to Euplatypus segnis and damaging the pecan wood were identified as: Helminthosporium sp., Aspergillus sp., Penicillium sp., Phoma sp., Ascochyta sp., Phaecylomices sp., Umbeliopsis sp., Torula sp., Fusarium solani, Alternaria alternata, Fusarum oxysporum, and Lasiodiplodia theobromae. The pathogenicity tests on healthy 3 year old pecan trees cv. western using Fusarium oxysporum, Fusarium solani, Alternaria alternata and Lasiodiplodia theobromae suspension conidia shown die back tree branches after 84 days inoculation. The insect in combination with the fungal invasion eventually cause the death of trees. Additionally, the insect contributes to the spread of fungi in pecan nut orchards.展开更多
The pecan nut is considered one of the most important dried fruits in the world by its classification as healthy food. The nutritional balance in the harvest is crucial for its impact on the production, quality and co...The pecan nut is considered one of the most important dried fruits in the world by its classification as healthy food. The nutritional balance in the harvest is crucial for its impact on the production, quality and content of bioactive compounds. This research was carried out in the city of Aldama, Chihuahua (Mexico), where doses of mineral and organic fertilization were tested in L25 Taguchi structure with 6 factors and 5 levels by factor: nitrogen (N) 0 - 240 kg·ha-1, phosphate (P2O5) 0 - 120 kg·ha-1, potassium (K2O) 0 - 100 kg·ha-1, calcium (CaO) 0 - 400 kg·ha-1, liquid humus 0 - 3600 L·ha-1 and solid humus 0 - 8000 kg·ha-1. An average yield of 2.2 t·ha-1 was obtained;145 nuts per kilogram, 60% of the edible part of the nut. The total phenolic content was 225.9 mg gallic acid g-1 and the antioxidant capacity was 180.9 mg Trolox g-1. It is concluded that the factor with the greatest impact on yield, quality and antioxidant capacity in pecan nut was nitrogen. It was also found that the combination of mineral and organic fertilization helps to maintain the production and quality ranges of the nut. In turn, these factors contribute to the increase in the phenolic strength and antioxidant capacity. The optimal fertilization dosis to obtain the maximum levels in production of 3.2 t·ha-1;decrease the number of nuts per kilogram to 135, increase the percentage of the edible part of the nut to 61.8%, the increase in the total phenolic content to 318.6 mg of gallic acid g-1 and in the antioxidant capacity with 187.2 mg of Trolox g-1, was 184 kg·ha-1 of N, 107.4 kg·ha-1 of P2O5, 50 kg·ha-1 of K2O y 2777 L·ha-1 of liquid humus. Finally, the mineral fertilization complemented with organic fertilization is considered a good fertilization strategy for pecan trees and its possible benefits to health and the environment.展开更多
目的分离纯化碧根果致敏原Car i 1,并对其结构进行表征鉴定。方法以新鲜碧根果果仁为原料,通过粉碎、脱脂、浸提、粗分级、凝胶过滤层析,对碧根果致敏原蛋白Car i 1进行分离纯化。结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、液相色谱-串...目的分离纯化碧根果致敏原Car i 1,并对其结构进行表征鉴定。方法以新鲜碧根果果仁为原料,通过粉碎、脱脂、浸提、粗分级、凝胶过滤层析,对碧根果致敏原蛋白Car i 1进行分离纯化。结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、液相色谱-串联质谱法和免疫印迹法3种方法对Cari1进行鉴定,并通过圆二色谱仪与紫外分光光度计表征其二、三级结构。结果本方法纯化获得碧根果致敏原Cari1,单轮制备量可达5 mg以上,且纯度大于95%,蛋白质高级结构未被破坏,能够被全部3名碧根果过敏患者的血清准确识别。结论该纯化方法技术路线简单、设备要求低且单次制备量高,总得率可达65%,操作便捷,为碧根果致敏原Car i 1的相关研究奠定了物质基础。展开更多
文摘In order to explore the forestation technique of pecan in Yunan, different types of pecan seedlings, bare-rooted seedlings and container seedlings were plant- ed in Yunnan, and their survival rates were investigated. This study will provide im- portant guidance for development of pecan industry in Yunnan Province.
文摘Euplatypus segnis is an insect pest of economic importance in pecan (Carya illinoensis) trees grown at Parras, General Cepeda and Torreón Coahuila, Mexico. The objectives in this study-were to identify the fungal strains associated to ambrosia borer body and diseased pecan wood and determine their pathogenicity. The results showed that the associated fungi to Euplatypus segnis and damaging the pecan wood were identified as: Helminthosporium sp., Aspergillus sp., Penicillium sp., Phoma sp., Ascochyta sp., Phaecylomices sp., Umbeliopsis sp., Torula sp., Fusarium solani, Alternaria alternata, Fusarum oxysporum, and Lasiodiplodia theobromae. The pathogenicity tests on healthy 3 year old pecan trees cv. western using Fusarium oxysporum, Fusarium solani, Alternaria alternata and Lasiodiplodia theobromae suspension conidia shown die back tree branches after 84 days inoculation. The insect in combination with the fungal invasion eventually cause the death of trees. Additionally, the insect contributes to the spread of fungi in pecan nut orchards.
文摘The pecan nut is considered one of the most important dried fruits in the world by its classification as healthy food. The nutritional balance in the harvest is crucial for its impact on the production, quality and content of bioactive compounds. This research was carried out in the city of Aldama, Chihuahua (Mexico), where doses of mineral and organic fertilization were tested in L25 Taguchi structure with 6 factors and 5 levels by factor: nitrogen (N) 0 - 240 kg·ha-1, phosphate (P2O5) 0 - 120 kg·ha-1, potassium (K2O) 0 - 100 kg·ha-1, calcium (CaO) 0 - 400 kg·ha-1, liquid humus 0 - 3600 L·ha-1 and solid humus 0 - 8000 kg·ha-1. An average yield of 2.2 t·ha-1 was obtained;145 nuts per kilogram, 60% of the edible part of the nut. The total phenolic content was 225.9 mg gallic acid g-1 and the antioxidant capacity was 180.9 mg Trolox g-1. It is concluded that the factor with the greatest impact on yield, quality and antioxidant capacity in pecan nut was nitrogen. It was also found that the combination of mineral and organic fertilization helps to maintain the production and quality ranges of the nut. In turn, these factors contribute to the increase in the phenolic strength and antioxidant capacity. The optimal fertilization dosis to obtain the maximum levels in production of 3.2 t·ha-1;decrease the number of nuts per kilogram to 135, increase the percentage of the edible part of the nut to 61.8%, the increase in the total phenolic content to 318.6 mg of gallic acid g-1 and in the antioxidant capacity with 187.2 mg of Trolox g-1, was 184 kg·ha-1 of N, 107.4 kg·ha-1 of P2O5, 50 kg·ha-1 of K2O y 2777 L·ha-1 of liquid humus. Finally, the mineral fertilization complemented with organic fertilization is considered a good fertilization strategy for pecan trees and its possible benefits to health and the environment.
文摘目的分离纯化碧根果致敏原Car i 1,并对其结构进行表征鉴定。方法以新鲜碧根果果仁为原料,通过粉碎、脱脂、浸提、粗分级、凝胶过滤层析,对碧根果致敏原蛋白Car i 1进行分离纯化。结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、液相色谱-串联质谱法和免疫印迹法3种方法对Cari1进行鉴定,并通过圆二色谱仪与紫外分光光度计表征其二、三级结构。结果本方法纯化获得碧根果致敏原Cari1,单轮制备量可达5 mg以上,且纯度大于95%,蛋白质高级结构未被破坏,能够被全部3名碧根果过敏患者的血清准确识别。结论该纯化方法技术路线简单、设备要求低且单次制备量高,总得率可达65%,操作便捷,为碧根果致敏原Car i 1的相关研究奠定了物质基础。
