Multiple mating by females,polyandry,is common in insects,including in tephritid fruit flies.Female insects that remate commonly store sperm of multiple males.How the sperm of different males contribute to paternity i...Multiple mating by females,polyandry,is common in insects,including in tephritid fruit flies.Female insects that remate commonly store sperm of multiple males.How the sperm of different males contribute to paternity is an important element of sexual selection.Sexual behavior and reproduction of the Queensland fruit fly(Qfly),Bactrocera tryoni,has been extensively investigated both in relation to understanding this economically important species’reproductive biology and in relation to implications for Sterile Insect Technique(SIT),whereby sterile flies are released to constrain reproduction of pest populations.Despite numerous studies of pre-and postcopulatory sexual selection in Qfly,there have been no direct studies of paternity patterns in polyandrous female Qflies.We used two morphologically distinguishable lines to investigate patterns of sperm use in Qfly.The two lines showed comparable mating performance evidenced by similar mating and remating frequency,copula duration,and proportion of second mate paternity(P2)between reciprocal crosses.The mechanism of sperm usage,with P2 close to 0.5 immediately after the second mating followed by gradual decrease of P2 as females aged,is most consistent with stratification or repositioning of sperm.Patterns observed in the present study are compared with the available information from other tephritid fruit flies,and are discussed in relation to this species’reproductive biology,known patterns of sperm storage,and SIT.展开更多
基金The authors thank Dr.Simon Baxter from the University of Melbourne(previously University of Adelaide)for facilitating the use of yellowr line Qfly colony.This research was conducted as part of the SITplus collaborative fruit fly program.Projeet Raising Q-ly Sterile Inseet Tech-nique to World Standard(HG14033)is funded by the Hort Frontiers Fruit Fly Fund,part of the Hort Frontiers strategic partnership initiative developed by Hort Innovation,with co-investment from Macquarie University and contributions from the Australian Government.JS was supported by a Macquarie University iRTP Scholarship.
文摘Multiple mating by females,polyandry,is common in insects,including in tephritid fruit flies.Female insects that remate commonly store sperm of multiple males.How the sperm of different males contribute to paternity is an important element of sexual selection.Sexual behavior and reproduction of the Queensland fruit fly(Qfly),Bactrocera tryoni,has been extensively investigated both in relation to understanding this economically important species’reproductive biology and in relation to implications for Sterile Insect Technique(SIT),whereby sterile flies are released to constrain reproduction of pest populations.Despite numerous studies of pre-and postcopulatory sexual selection in Qfly,there have been no direct studies of paternity patterns in polyandrous female Qflies.We used two morphologically distinguishable lines to investigate patterns of sperm use in Qfly.The two lines showed comparable mating performance evidenced by similar mating and remating frequency,copula duration,and proportion of second mate paternity(P2)between reciprocal crosses.The mechanism of sperm usage,with P2 close to 0.5 immediately after the second mating followed by gradual decrease of P2 as females aged,is most consistent with stratification or repositioning of sperm.Patterns observed in the present study are compared with the available information from other tephritid fruit flies,and are discussed in relation to this species’reproductive biology,known patterns of sperm storage,and SIT.
