This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as subs...This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as substrates. Bacillus megaterium HDYM-09 produced pectin lyase that exhibited the highest activity of 2116.71 ± 11.55 U/mL. Bacillus licheniformis HDYM-03 produced pectate lyase that exhibited the highest activity of 611.21 ± 14.54 U/mL. Based on these fi ndings, we constructed four retting systems to degrade the pectin substance. The results showed that the content of galacturonic acid in the mixed system was 529.21 μg/mL, the content of reducing sugar was 98.14 mg/mL, and the weight loss ratio of cells reached 19.49%, which were signifi cantly higher than those in other systems. The mixed system has more advantages, and the utilization rate of degumming was higher, which further ensured that the degumming can be carried out effi ciently and quickly. The mixed system exhibits feasible applications in the fi ber and textile industry.展开更多
Nodular structures were formed by rhizobia on oilseed rape roots following treatment with pectinolytic bacteria. Nodules developed within 50 days.Photomicrograph of nodule cells showed that the capsulated bacteria wer...Nodular structures were formed by rhizobia on oilseed rape roots following treatment with pectinolytic bacteria. Nodules developed within 50 days.Photomicrograph of nodule cells showed that the capsulated bacteria were intrarellular.Rhizobia reisolated from the root nodules retained not only the ability of nodulation but also the characteristic of resistance to 100 μg neomycin mL-1.A low nitrogenaSe activity of the nodules was determined by the method of acetylene reduction.展开更多
[Objectives] This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No. 1 as the test material.[Methods]Huanyou No. 1 was sprayed with chitin,and the changes of som...[Objectives] This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No. 1 as the test material.[Methods]Huanyou No. 1 was sprayed with chitin,and the changes of some physiological and biochemical indexes in fruit softening were investigated. [Results]Chitin treatment significantly improved single fruit weight,increased soluble solid content,but decreased titratable acid content and significantly reduced the content of cellulose and hemicellulose; and the chitin treatment significantly inhibited the breakdown of pectin,cellulose and hemicellulose,and inhibited the activities of PG enzyme and β-Gal enzyme. [Conclusions] Chitin spray treatment could increase the yield of A. arguta,and improve its quality and storage property.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 31270534, 31770538)the National Natural Science Youth Foundation of China (No. 31300355)the Post-Doctorate Foundation of Heilongjiang Province (No. LBH-Z15214)
文摘This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as substrates. Bacillus megaterium HDYM-09 produced pectin lyase that exhibited the highest activity of 2116.71 ± 11.55 U/mL. Bacillus licheniformis HDYM-03 produced pectate lyase that exhibited the highest activity of 611.21 ± 14.54 U/mL. Based on these fi ndings, we constructed four retting systems to degrade the pectin substance. The results showed that the content of galacturonic acid in the mixed system was 529.21 μg/mL, the content of reducing sugar was 98.14 mg/mL, and the weight loss ratio of cells reached 19.49%, which were signifi cantly higher than those in other systems. The mixed system has more advantages, and the utilization rate of degumming was higher, which further ensured that the degumming can be carried out effi ciently and quickly. The mixed system exhibits feasible applications in the fi ber and textile industry.
文摘Nodular structures were formed by rhizobia on oilseed rape roots following treatment with pectinolytic bacteria. Nodules developed within 50 days.Photomicrograph of nodule cells showed that the capsulated bacteria were intrarellular.Rhizobia reisolated from the root nodules retained not only the ability of nodulation but also the characteristic of resistance to 100 μg neomycin mL-1.A low nitrogenaSe activity of the nodules was determined by the method of acetylene reduction.
文摘[Objectives] This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No. 1 as the test material.[Methods]Huanyou No. 1 was sprayed with chitin,and the changes of some physiological and biochemical indexes in fruit softening were investigated. [Results]Chitin treatment significantly improved single fruit weight,increased soluble solid content,but decreased titratable acid content and significantly reduced the content of cellulose and hemicellulose; and the chitin treatment significantly inhibited the breakdown of pectin,cellulose and hemicellulose,and inhibited the activities of PG enzyme and β-Gal enzyme. [Conclusions] Chitin spray treatment could increase the yield of A. arguta,and improve its quality and storage property.
