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OsTHA8 encodes a pentatricopeptide repeat protein required for RNA editing and splicing during rice chloroplast development
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作者 Yanwei Wang Yu Duan Pengfei Ai 《The Crop Journal》 SCIE CSCD 2023年第5期1353-1367,共15页
In higher plants, the chloroplast is the most important organelle for photosynthesis and for numerous essential metabolic processes in the cell. Although many genes involved in chloroplast development have been identi... In higher plants, the chloroplast is the most important organelle for photosynthesis and for numerous essential metabolic processes in the cell. Although many genes involved in chloroplast development have been identified, the mechanisms underlying such development are not fully understood. In this study, a rice(Oryza sativa) mutant exhibiting pale green color and seedling lethality was isolated from a mutant library. The mutated gene was identified as an ortholog of THA8(thylakoid assembly 8) in Arabidopsis and maize. This gene is designated as OsTHA8 hereafter. OsTHA8 showed a typical pentatricopeptide repeat(PPR) characteristic of only four PPR motifs. Inactivation of OsTHA8 led to a deficiency in chloroplast development in the rice seedling stage. OsTHA8 was expressed mainly in young leaves and leaf sheaths.The OsTHA8 protein was localized to the chloroplast. Loss of function of OsTHA8 weakened the editing efficiency of ndhB-611/737 and rps8-182 transcripts under normal conditions. Y2H and BiFC indicated that OsTHA8 facilitates RNA editing by forming an editosome with multiple organellar RNA editing factor(OsMORF8) and thioredoxin z(OsTRXz), which function in RNA editing in rice chloroplasts. Defective OsTHA8 impaired chloroplast ribosome assembly and resulted in reduced expression of PEP-dependent genes and photosynthesis-related genes. Abnormal splicing of the chloroplast gene ycf3 was detected in ostha8. These findings reveal a synergistic regulatory mechanism of chloroplast biogenesis mediated by RNA, broaden the function of the PPR family, and shed light on the RNA editing complex in rice. 展开更多
关键词 Oryza sativa L. Chloroplast biogenesis pentatricopeptide repeat protein RNA editing RNA splicing
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CsPPR和CsCPN60-like在茶树白化叶片中的表达分析及互作蛋白验证
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作者 王涛 漆思雨 +6 位作者 韦朝领 王艺清 戴浩民 周喆 曹士先 曾雯 孙威江 《生物技术通报》 CAS CSCD 北大核心 2023年第3期218-231,共14页
本课题组前期通过转录组筛选出2个与‘白鸡冠’茶树(Camellia sinensis)叶片白化相关的基因(CSS0013384和CSS0036305),为探明CSS0013384和CSS0036305在白化茶树中的表达模式与其互作蛋白,以‘白鸡冠’茶树叶片为材料,克隆CSS0013384和CS... 本课题组前期通过转录组筛选出2个与‘白鸡冠’茶树(Camellia sinensis)叶片白化相关的基因(CSS0013384和CSS0036305),为探明CSS0013384和CSS0036305在白化茶树中的表达模式与其互作蛋白,以‘白鸡冠’茶树叶片为材料,克隆CSS0013384和CSS0036305 cDNA全长序列,利用生物信息学、酵母单杂交和酵母双杂交,分析其蛋白理化性质、系统进化树、染色体定位、基因结构、蛋白结构、蛋白调控与互作网络和基因表达模式。生物信息学分析结果表明,CSS0013384和CSS0036305分别属于三角状五肽蛋白(pentatricopeptide repeat protein,PPR)和伴侣蛋白(chaperone,CPN60-like)家族,其蛋白质编码区(coding sequence,CDS)长度为1893 bp和1752 bp,编码氨基酸个数为631和575,蛋白质质量为71.87 kD和60.79 kD,等电点为8.93和6.21。亚细胞定位预测结果表明,CSS0013384定位于叶绿体,CSS0036305定位于线粒体。通过白鸡冠茶树第二叶的遮阴和恢复光照处理与不同叶色茶树品种的RT-qPCR发现,CSS0013384和CSS0036305在白化芽叶中高表达。CSS0002807属于PIF转录因子家族,酵母单杂交结果表明CSS0002807可以结合CSS0013384启动子。CSS0013384和CSS0036305在白化茶树叶片中可能参与叶绿体和线粒体发育,在叶片白化过程中发挥重要作用,结果可为进一步探究茶树叶片白化机理提供参考。 展开更多
关键词 茶树 白化 三角状五肽蛋白 伴侣蛋白 表达分析 互作蛋白
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马铃薯PPR蛋白家族基因SoDIPPR的克隆及其在干旱条件下的表达特征分析 被引量:8
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作者 范敏 金黎平 +1 位作者 刘庆昌 屈冬玉 《中国农业科学》 CAS CSCD 北大核心 2008年第8期2249-2257,共9页
【目的】PPR(pentatricope ptide repeat)蛋白家族在植物的生长发育、细胞器形成、细胞质雄性不育的育性恢复、RNA的编辑加工、细胞核与细胞器之间信号传递、逆境防御等方面具有重要作用。研究旨在了解PPR蛋白家族SoDIPPR基因在马铃薯... 【目的】PPR(pentatricope ptide repeat)蛋白家族在植物的生长发育、细胞器形成、细胞质雄性不育的育性恢复、RNA的编辑加工、细胞核与细胞器之间信号传递、逆境防御等方面具有重要作用。研究旨在了解PPR蛋白家族SoDIPPR基因在马铃薯中的表达情况及其在干旱胁迫下的作用【。方法】以抗旱马铃薯二倍体品系H145为材料,利用cDNA-AFLP技术寻找与抗旱性密切相关的基因cDNA片段,利用RACE技术克隆其基因全长cDNA,并利用半定量RT-PCR和Northern杂交法研究其在干旱条件下的表达情况。【结果】从抗旱马铃薯二倍体品系H145中克隆了一个836bp全长cDNA序列,命名为SoDIPPR,该序列开放阅读框长为588bp,编码195个氨基酸序列。序列分析表明,SoDIPPR蛋白存在PPR结构域、激酶结合区、和C端SMR(small MutS related protein)区域。SoDIPPR蛋白序列与GenBank数据库中的其它PPR蛋白进行同源序列比对,构建系统进化树,发现SoDIPPR与其它14个高等植物的PPR蛋白同源性为57%~82%,与苜蓿DNA错配修复蛋白MuTs2、水稻盐诱导蛋白(Q9LS25)和叶绿体RNA结合蛋白(Q75IP8)的同源性达69%~82%。半定量RT-PCR和Northern杂交结果表明,SoDIPPR基因在干旱胁迫下叶片和根系里的表达量明显增加,说明SoDIPPR基因在马铃薯抗旱中起一定作用。在持续干旱条件下,SoDIPPR基因在抗旱品系H145与干旱敏感品系H214中表达模式不同。【结论】马铃薯SoDIPPR基因编码的蛋白与其它植物PPR家族蛋白同源性较高,SoDIPPR基因参与马铃薯对干旱胁迫的应答反应,可能对抵抗干旱胁迫有一定作用。 展开更多
关键词 马铃薯(Solarium TUBEROSUM L.) 抗旱性 全长CDNA ppr(pentat ricopeptide repeat)蛋白 克隆 表达
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PPR蛋白调控植物细胞器RNA加工的分子功能 被引量:3
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作者 李秀兰 姜曰水 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2018年第7期713-718,共6页
35肽重复(pentatricopeptide repeat,PPR)蛋白是2000年发现的一类由多个重复单位串联而成的核编码蛋白质。PPR蛋白广泛存在于真核生物中,在陆生植物中尤为普遍。PPR蛋白大多定位于线粒体或叶绿体。多项研究表明,PPR蛋白为序列特异性RNA... 35肽重复(pentatricopeptide repeat,PPR)蛋白是2000年发现的一类由多个重复单位串联而成的核编码蛋白质。PPR蛋白广泛存在于真核生物中,在陆生植物中尤为普遍。PPR蛋白大多定位于线粒体或叶绿体。多项研究表明,PPR蛋白为序列特异性RNA结合蛋白质,在细胞器RNA编辑、剪接、稳定、切割及翻译等转录后加工过程发挥重要作用。PPR蛋白功能缺陷会导致植物生长发育异常,甚至胚胎致死。本文主要就PPR蛋白功能及作用机制进行综述,并对尚待解决的问题及研究前景加以探讨,以期为PPR蛋白的深入研究提供思路。 展开更多
关键词 ppr蛋白 叶绿体 线粒体 RNA加工
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PPR蛋白在植物线粒体和叶绿体中功能的研究进展 被引量:2
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作者 王婉珍 田发安 +1 位作者 任育军 缪颖 《福建农林大学学报(自然科学版)》 CSCD 北大核心 2018年第3期257-266,共10页
植物中的线粒体和叶绿体是包含遗传信息的半自主性细胞器,它们的功能同时受到自身和细胞核遗传信息共同的调控.三角状五肽(PPR)蛋白是一类含有三角状五肽重复结构的核基因组编码蛋白的大家族.植物中的PPR蛋白通常定位于线粒体或叶绿体中... 植物中的线粒体和叶绿体是包含遗传信息的半自主性细胞器,它们的功能同时受到自身和细胞核遗传信息共同的调控.三角状五肽(PPR)蛋白是一类含有三角状五肽重复结构的核基因组编码蛋白的大家族.植物中的PPR蛋白通常定位于线粒体或叶绿体中,并对这些细胞器基因组转录的RNA进行编辑和修饰,参与许多重要的组织发生和器官形成过程的调控,同时也参与了植物对内外环境变化过程的响应等.因此,PPR是研究植物中细胞器功能和核质互作机制的热点.依据PPR蛋白基序的种类和排列方式,将植物中的PPR分为两大亚类:P类和PLS类.P类由经典的35个氨基酸基序排列组成,主要参与细胞器基因的转录调控;短的S、长的L和经典的P基序排列组成PLS亚族,主要对细胞器基因转录的RNA进行编辑修饰.本文主要从PPR蛋白的结构、亚细胞定位以及在线粒体和叶绿体中的功能等方面着手阐述植物PPR蛋白在叶绿体和线粒体基因组转录和加工过程中的作用以及与植物发育之间的关系,并对研究中存在的一些问题提出设想. 展开更多
关键词 三角状五肽(ppr)蛋白 线粒体 叶绿体 亚细胞定位 RNA加工 植物发育
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EMBRYONIC FACTOR 19 Encodes a Pentatricopeptide Repeat Protein that is Essential for the Initiation of Zygotic Embryogenesis in Arabidopsis 被引量:8
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作者 Dali Yu Li Jiang +1 位作者 Huaqin Gong Chun-Ming Liu 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2012年第1期55-64,共10页
Early embryogenesis is the most fundamental developmental process in biology. Screening of ethyl methanesulfonate (EMS)-mutagenized populations of Arabidopsis thaliana led to the identification of a zygote-lethal mu... Early embryogenesis is the most fundamental developmental process in biology. Screening of ethyl methanesulfonate (EMS)-mutagenized populations of Arabidopsis thaliana led to the identification of a zygote-lethal mutant embryonic factor 19 (fac19) in which embryo development was arrested at the elongated zygote to octant stage. The number of endosperm nuclei decreased significantly in fac19 embryos. Genetic analysis showed fac19 was caused by a single recessive mutation with typical mendelian segregation, suggesting equal maternal and paternal contributions of FAC19 towards zygotic embryogenesis. Positional cloning showed that FAC19 encodes a putative mitochondrial protein with 16 conserved pentatricopeptide repeat (PPR) motifs. The fac19 mutation caused a conversion from hydrophilic serine located in a previously unknown domain to hydrophobic leucine. Crosses between FAC191fac19 and the T-DNA insertion mutants in the same gene failed to complement the fac19 defects, confirming the identity of the gene. This study revealed the critical importance of a PPR protein-mediated mitochondrial function in early embryogenesis. 展开更多
关键词 EMBRYOGENESIS mitochondrial proteins pentatricopeptide repeat motifs zygote activation.
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OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development 被引量:7
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作者 Dekai Wang Heqin Liu +3 位作者 Guowei Zhai Liangsheng Wang Jianfeng Shao Yuezhi Tao 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2016年第10期601-608,共8页
Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice(Oryza sativa) mut... Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice(Oryza sativa) mutant that exhibited albino and seedling-lethal phenotypes was isolated from a60Co-irradiated rice population. The mutant gene was identified as an ortholog of the Arabidopsis plastid transcriptionally active chromosome protein 2(p TAC2) gene, and the mutant strain was designated osptac2. Sequence and transcription analyses showed that Osp TAC2 encodes a putative chloroplast protein consisting of 10 pentratricopeptide repeat(PPR) domains and a C-terminal small Mut S-related(SMR) domain. Cytological observations via microscopy showed that the Osp TAC2-green fluorescent fusion protein is localized in the chloroplasts. Transmission electron microscopy revealed that the chloroplast of the osptac2 mutant lacks an organized thylakoid membrane. The transcript levels of all investigated PEP(plastid-encoded RNA polymerase)-dependent genes were dramatically reduced in the osptac2 mutant, whereas the transcript levels of NEP(nuclear-encoded polymerase)-dependent genes were increased. These results suggest that Osp TAC2 plays a critical role in chloroplast development and indicate that the molecular function of the Osp TAC2 gene is conserved in rice and Arabidopsis. 展开更多
关键词 Oryza sativa pentatricopeptide repeat protein Chloroplast development
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CDE4 encodes a pentatricopeptide repeat protein involved in chloroplast RNA splicing and affects chloroplast development under low-temperature conditions in rice 被引量:3
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作者 Xinyong Liu Xichun Zhang +8 位作者 Ruijie Cao Guiai Jiao Shikai Hu Gaoneng Shao Zhonghua Sheng Lihong Xie Shaoqing Tang Xiangjin Wei Peisong Hu 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2021年第10期1724-1739,共16页
Pentatricopeptide repeat(PPR)proteins play important roles in the post-transcriptional modification of organellar RNAs in plants.However,the function of most PPR proteins remains unknown.Here,we characterized the rice... Pentatricopeptide repeat(PPR)proteins play important roles in the post-transcriptional modification of organellar RNAs in plants.However,the function of most PPR proteins remains unknown.Here,we characterized the rice(Oryza sativa L.)chlorophyll deficient 4(cde4)mutant which exhibits an albino phenotype during early leaf development,with decreased chlorophyll contents and abnormal chloroplasts at low-temperature(20℃).Positional cloning revealed that CDE4 encodes a P-type PPR protein localized in chloroplasts.In the cde4 mutant,plastid-encoded polymerase(PEP)-dependent transcript levels were significantly reduced,but transcript levels of nuclear-encoded genes were increased compared to wild-type plants at 20℃.CDE4 directly binds to the transcripts of the chloroplast genes rpl2,ndhA,and ndhB.Intron splicing of these transcripts was defective in the cde4 mutant at 20℃,but was normal at 32℃.Moreover,CDE4 interacts with the guanylate kinase VIRESCENT 2(V2);overexpression of V2 enhanced CDE4 protein stability,thereby rescuing the cde4 phenotype at 20℃.Our results suggest that CDE4 participates in plastid RNA splicing and plays an important role in rice chloroplast development under lowtemperature conditions. 展开更多
关键词 CDE4 chloroplast development pentatricopeptide repeat(ppr)protein RNA splicing LOW-TEMPERATURE Oryza sativa L.
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A Small Multifunctional Pentatricopeptide Repeat Protein in the Chloroplast of Chlamydomonas reinhardtii
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作者 Abdullah Jala Christian Schwarz +3 位作者 Christian Schmitz-Linneweber Olivier Vallon Jorg Nickelsen Alexandra-Viola Bohne 《Molecular Plant》 SCIE CAS CSCD 2015年第3期412-426,共15页
Organellar biogenesis is mainly regulated by nucleus-encoded factors, which act on various steps of gene expression including RNA editing, processing, splicing, stabilization, and translation initiation. Among these r... Organellar biogenesis is mainly regulated by nucleus-encoded factors, which act on various steps of gene expression including RNA editing, processing, splicing, stabilization, and translation initiation. Among these regulatory factors, pentatricopeptide repeat (PPR) proteins form the largest family of RNA binding proteins, with hundreds of members in flowering plants. In striking contrast, the genome of the unicellular green alga Chlamydomonas reinhardtii encodes only 14 such proteins. In this study, we analyzed PPR7, the smallest and most highly expressed PPR protein in C. reinhardtii. Green fluorescent protein-based localization and gel-filtration analysis revealed that PPR7 forms a part of a high-molecular-weight ribonu- cleoprotein complex in the chloroplast stroma. RIP-chip analysis of PPRT-bound RNAs demonstrated that the protein associates with a diverse set of chloroplast transcripts in vivo, i.e. rrnS, psbH, rpoC2, rbcL, atpA, cemA-atpH, tscA, and atpl-psaJ. Furthermore, the investigation of PPR7 RNAi strains revealed that deple- tion of PPR7 results in a light-sensitive phenotype, accompanied by altered levels of its target RNAs that are compatible with the defects in their maturation or stabilization. PPR7 is thus an unusual type of small multi- functional PPR protein, which interacts, probably in conjunction with other RNA binding proteins, with numerous target RNAs to promote a variety of post-transcriptional events. 展开更多
关键词 pentatricopeptide repeat protein chloroplast gene expression RNA binding protein RNA maturation RNA stabilization RIP-chip
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The pentatricopeptide repeat protein EMB1270 interacts with CFM2 to splice specific group Ⅱ introns in Arabidopsis chloroplasts
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作者 Li Zhang Jingli Chen +9 位作者 Liqun Zhang Ying Wei Yajuan Li Xinyun Xu Hui Wu Zhong‐Nan Yang Jirong Huang Fenhong Hu Weihua Huang Yong‐Lan Cui 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2021年第11期1952-1966,共15页
Chloroplast biogenesis requires the coordinated expression of chloroplast and nuclear genes.Here, we show that EMB1270, a plastid-localized pentatricopeptide repeat(PPR) protein, is required for chloroplast biogenesis... Chloroplast biogenesis requires the coordinated expression of chloroplast and nuclear genes.Here, we show that EMB1270, a plastid-localized pentatricopeptide repeat(PPR) protein, is required for chloroplast biogenesis in Arabidopsis thaliana.Knockout of EMB1270 led to embryo arrest,whereas a mild knockdown mutant of EMB1270 displayed a virescent phenotype. Almost no photosynthetic proteins accumulated in the albino emb1270 knockout mutant. By contrast, in the emb1270 knockdown mutant, the levels of ClpP1 and photosystem I(PSI) subunits were significantly reduced, whereas the levels of photosystem II(PSII) subunits were normal. Furthermore, the splicing efficiencies of the clpP1.2,ycf3.1, ndhA, and ndhB plastid introns were dramatically reduced in both emb1270 mutants. RNA immunoprecipitation revealed that EMB1270 associated with these introns in vivo. In an RNA electrophoretic mobility shift assay(REMSA), a truncated EMB1270 protein containing the 11 Nterminal PPR motifs bound to the predicted sequences of the clpP1.2, ycf3.1, and ndhA introns.In addition, EMB1270 specifically interacted with CRM Family Member 2(CFM2). Given that CFM2 is known to be required for splicing the same plastid RNAs, our results suggest that EMB1270 associates with CFM2 to facilitate the splicing of specific group II introns in Arabidopsis. 展开更多
关键词 Arabidopsis thaliana CRM Family Member 2 CHLOROPLAST EMB1270 intron splicing pentatricopeptide repeat protein
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油菜野芥细胞质雄性不育恢复基因候选片段的克隆 被引量:4
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作者 郝建轶 李云昌 +3 位作者 胡琼 梅德圣 李英德 徐育松 《中国油料作物学报》 CAS CSCD 北大核心 2011年第5期433-437,共5页
根据植物细胞质雄性不育恢复基因编码的PPR(pentatricopeptide repeat)蛋白的特点,结合拟南芥PPR基因簇相似序列,扩增油菜野芥细胞质雄性不育(Nsa CMS)基因组DNA,筛选出一对简并引物,在Nsa不育系育性恢复后的可育材料和野芥亲本中可以... 根据植物细胞质雄性不育恢复基因编码的PPR(pentatricopeptide repeat)蛋白的特点,结合拟南芥PPR基因簇相似序列,扩增油菜野芥细胞质雄性不育(Nsa CMS)基因组DNA,筛选出一对简并引物,在Nsa不育系育性恢复后的可育材料和野芥亲本中可以同时扩增出符合预期的片段。片段长度为309bp,与萝卜CMS恢复基因核苷酸序列的一致性为69%,与拟南芥1号染色体臂上PPR基因簇核苷酸序列的一致性大于70%,与含波里马(Pol)CMS恢复基因的白菜型油菜相关序列一致性达85%。该片段编码的氨基酸序列含有两个相邻排列的PPR基序,可作为Nsa CMS育性恢复基因的候选片段。 展开更多
关键词 甘蓝型油菜 野芥细胞质雄性不育 恢复基因 ppr蛋白 简并引物 基因克隆
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植物线粒体蛋白质组与细胞质雄性不育研究进展 被引量:6
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作者 郭宝健 陈丙堂 刘保申 《分子植物育种》 CAS CSCD 2007年第F11期111-117,共7页
细胞质雄性不育(cytoplasmic male sterility,CMS)是植物杂种优势利用的基础,大量研究表明CMS是线粒体基因与细胞核基因互作的结果。蛋白质是基因表达的产物,也是基因功能的执行者,研究线粒体蛋白质有利于探索CMS产生机理。本文综述了CM... 细胞质雄性不育(cytoplasmic male sterility,CMS)是植物杂种优势利用的基础,大量研究表明CMS是线粒体基因与细胞核基因互作的结果。蛋白质是基因表达的产物,也是基因功能的执行者,研究线粒体蛋白质有利于探索CMS产生机理。本文综述了CMS相关的线粒体蛋白质的研究进展,并探讨了PPR(penta- tricopeptide repeats)基因的结构特征、生物学功能及对CMS相关基因的表达调控。 展开更多
关键词 细胞质雄性不育性 线粒体蛋白质组 ppr
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The ABA-Deficiency Suppressor Locus HAS2 Encodes the PPR Protein LOI1/MEF11 Involved in Mitochondrial RNA Editing 被引量:1
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作者 Julien Sechet Camille Roux +9 位作者 Anne Plessis Delphine Effroy Anne Frey Francois Perreau Catherine Biniek Anja Krieger-Liszkay David Macherel Helen M. North Hakim Mireau Annie Marion-Poll 《Molecular Plant》 SCIE CAS CSCD 2015年第4期644-656,共13页
The hotABA-deficiency suppressor2 (has2) mutation increases drought tolerance and the ABA sensitivity of stomata closure and seed germination. Here we report that the HAS2 locus encodes the MITOCHONDRIAL EDITING FAC... The hotABA-deficiency suppressor2 (has2) mutation increases drought tolerance and the ABA sensitivity of stomata closure and seed germination. Here we report that the HAS2 locus encodes the MITOCHONDRIAL EDITING FACTOR11 (MEF11), also known as LOVASTATIN INSENSITIVE1. has21mef11 mutants exhibited phenotypes very similar to the ABA-hypersensitive mutant, hail-1 pp2ca-1 hab 1-1 abil-2, which is impaired in four genes encoding type 2C protein phosphatases (PP2C) that act as upstream negative regulators of the ABA signaling cascade. Like pp2c, mef11 plants were more resistant to progressive water stress and seed germination was more sensitive to paclobutrazol (a gibberellin biosynthesis inhibitor) as well as mannitol and NaCI, compared with the wild-type plants. Phenotypic alterations in mef11 were associated with the lack of editing of transcripts for the mitochondrial cytochrome c maturation FN2 (cCmFN2) gene, which encodes a cytochrome c-heme lyase subunit involved in cytochrome c biogenesis. Although the abundance of electron transfer chain complexes was not affected, their dysfunction could be deduced from increased respiration and altered production of hydrogen peroxide and nitric oxide in reef11 seeds. As minor defects in mitochondrial respiration affect ABA signaling, this suggests an essential role for ABA in mitochondrial retrograde regulation. 展开更多
关键词 abscisic acid mitochondria pentatricopeptide repeat protein RNA editing drought tolerance germination
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三角形五肽重复蛋白研究进展 被引量:3
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作者 黄俊然 黄文超 《湖北农业科学》 2018年第23期19-23,31,共6页
三角形五肽重复(PPR)蛋白是植物中一个庞大的蛋白家族,能够从RNA剪切、编辑、降解和翻译等多个方面影响细胞器中RNA的新陈代谢,PPR蛋白由连续的PPR基序构成,蛋白呈右手螺旋结构,具有与RNA单链结合的能力。随着研究的深入,人工设计合成PP... 三角形五肽重复(PPR)蛋白是植物中一个庞大的蛋白家族,能够从RNA剪切、编辑、降解和翻译等多个方面影响细胞器中RNA的新陈代谢,PPR蛋白由连续的PPR基序构成,蛋白呈右手螺旋结构,具有与RNA单链结合的能力。随着研究的深入,人工设计合成PPR蛋白技术已经成熟,PPR蛋白与RNA结合的规律已较为清晰,人工PPR蛋白有望被开发成为细胞器中介导RNA调控的新型生物技术。 展开更多
关键词 三角形五肽重复蛋白 RNA单链结合 细胞器RNA调控
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哺乳动物三角状五肽重复蛋白家族在线粒体基因表达调控中的研究进展
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作者 刘如爱 余敏 熊伟 《基因组学与应用生物学》 CAS CSCD 北大核心 2023年第5期449-460,共12页
三角状五肽重复(pentatricopeptide repeat,PPR)蛋白通常包含2~27个串联重复的结构域,每个结构域含有35个氨基酸残基。PPR蛋白是一类广泛存在于真核生物体内的RNA结合蛋白,对单链RNA具有序列特异性识别模式,与RNA的转录、剪切、编辑、... 三角状五肽重复(pentatricopeptide repeat,PPR)蛋白通常包含2~27个串联重复的结构域,每个结构域含有35个氨基酸残基。PPR蛋白是一类广泛存在于真核生物体内的RNA结合蛋白,对单链RNA具有序列特异性识别模式,与RNA的转录、剪切、编辑、稳定性及翻译等过程密切相关。迄今为止,在哺乳动物中仅发现7个PPR蛋白,它们均存在于线粒体中,包括线粒体RNA聚合酶(mitochondrial RNA polymerase,POLRMT)、富含亮氨酸的三角状五肽重复结构蛋白(leucinerich pentatricopeptide repeat-containing protein,LRPPRC)蛋白、线粒体核糖体小亚基蛋白27(mitochondrial ribosomal protein of the small subunit 27,MRPS27)、线粒体核糖核酸酶P蛋白3(mitochondrial RNase P protein 3,MRPP3)以及五肽重复结构域蛋白(pentaricopeptide repeat domain protein,PTCD)1~3。哺乳动物PPR蛋白家族的成员在线粒体转录、RNA代谢和翻译中具有多种调控作用,对于线粒体功能和细胞健康至关重要。本文简要概述了哺乳动物细胞中PPR蛋白家族7个成员的结构和亚细胞定位,探讨了PPR蛋白在线粒体基因表达调控中的作用,并展望了PPR蛋白对人类线粒体相关疾病的应用前景。 展开更多
关键词 哺乳动物线粒体 三角状五肽重复蛋白 线粒体RNA加工 线粒体翻译
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假禾谷镰孢中三角状五肽重复蛋白的全基因组鉴定与表达分析 被引量:2
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作者 王利民 孟家兴 +6 位作者 庄训宇 张媛 李海洋 陈琳琳 汪敏 丁胜利 李洪连 《植物病理学报》 CAS CSCD 北大核心 2022年第3期341-351,共11页
假禾谷镰孢(Fusarium pseudograminearum,Fp)是引起小麦茎基腐病的优势病原菌,但关于该病原菌致病机理的研究报道很少。三角状五肽重复蛋白(Pentatricopeptide repeat protein,Ppr)为一类核编码的RNA结合蛋白,通过结合特定的RNA序列调... 假禾谷镰孢(Fusarium pseudograminearum,Fp)是引起小麦茎基腐病的优势病原菌,但关于该病原菌致病机理的研究报道很少。三角状五肽重复蛋白(Pentatricopeptide repeat protein,Ppr)为一类核编码的RNA结合蛋白,通过结合特定的RNA序列调控靶标基因RNA的成熟过程,参与线粒体基因组转录和翻译。Ppr蛋白在植物、动物(包括人)及酵母中有一些研究报道,但在丝状真菌特别是植物病原真菌中鲜见报道。为了明确假禾谷镰孢中PPR基因家族的特征,本研究通过全基因组Blastp分析,共鉴定出8个PPR候选基因序列FpPPR1~FpPPR8,主要分布在1号、3号、4号染色体上,基本不含有内含子。理化性质与功能预测显示,Ppr为亲水性蛋白,氨基酸长度510~1353 aa,分子量59.09~152.23 kDa,等电点为5.23~9.69,其中FpPpr3和FpPpr7为稳定蛋白,其他6个蛋白为不稳定蛋白。亚细胞定位预测显示,Ppr蛋白主要位于线粒体。FpPpr1、FpPpr3、FpPpr5、FpPpr6和FpPpr7的3D结构预测形成明显的超螺旋结构。在假禾谷镰孢营养阶段和侵染过程的转录组数据中分析了8个基因表达模式,通过qRT-PCR进行验证,FpPPR1、FpPPR3、FpPPR5在菌丝阶段高表达,FpPPR1、FpPPR5在侵染后期显著下调。本研究结果为进一步研究假禾谷镰孢中PPRs基因的生物学功能奠定基础。 展开更多
关键词 小麦茎基腐病 假禾谷镰孢 三角状五肽重复蛋白(ppr) Blastp分析 表达模式
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龙眼miR403及其候选靶标对外源激素的响应模式以及在龙眼体胚中的表达模式 被引量:4
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作者 苏立遥 黄倏祺 +5 位作者 蒋梦琦 厉雪 徐小萍 陈旭 赖钟雄 林玉玲 《应用与环境生物学报》 CAS CSCD 北大核心 2019年第4期977-984,共8页
MicroRNA403(miR403)为双子叶植物特有的miRNA家族,其在双子叶植物抗病、逆境胁迫和生长发育中具有重要作用.为了解miR403及其候选靶标对外源激素的响应模式以及在龙眼体胚发生过程中的表达模式,利用psRNAtarget对miR403潜在靶标进行预... MicroRNA403(miR403)为双子叶植物特有的miRNA家族,其在双子叶植物抗病、逆境胁迫和生长发育中具有重要作用.为了解miR403及其候选靶标对外源激素的响应模式以及在龙眼体胚发生过程中的表达模式,利用psRNAtarget对miR403潜在靶标进行预测,采用改良RLM-RACE技术验证其裂解位点,通过qPCR技术检测miR403及其候选靶标对外源激素的应答及在龙眼体胚中的表达模式.结果显示,共获得41个龙眼miR403的潜在靶标,包含4个PPR(Pentatricopeptide repeat protein)和3个PCNT115(Auxin-induced protein PCNT115),却未发现AGO2(Argonaute2).4个PPR中Dlo014588.1和Dlo014589.1序列一致,而3个PCNT115基因序列在所预测的miR403结合位点上下游序列基本一致.裂解位点显示,miR403不具备裂解AGO2 mRNA的能力,但介导PPR(Dlo014588.1)和PCNT115 mRNA的裂解,裂解位点位于miR403 5′端的第2和第3个碱基之间. qPCR显示,miR403响应脱落酸(ABA)信号并显著上调,PPR和PCNT115对不同浓度的ABA呈现不同的表达模式,PPR和PCNT115在5μg/L ABA时显著上调,随后,PPR先显著下调(50μg/L ABA),而后显著上调(5 000μg/L ABA),而PCNT115呈显著下调趋势;miR403不响应赤霉素(GA3)信号,而PPR和PCNT115随着GA3浓度升高而上调;miR403可以响应不同浓度的水杨酸(SA)和2,4-D信号显著下调,而其靶基因显著上调.不同胚性培养物中qPCR显示,miR403仅在龙眼胚性愈伤组织(EC)高度表达且在龙眼体胚发生过程中显著下调,PPR在EC和子叶胚(CE)高度表达,PCNT115在CE和成熟胚(ME)高水平表达,三者之间并未呈现明显的负调控趋势.本研究表明在龙眼体胚中miR403并不靶向调控AGO2,而是调控PPR和PCNT115;另外,miR403可能响应ABA、SA和2,4-D调控PPR和PCNT15参与到龙眼体胚发生过程中. 展开更多
关键词 龙眼 miR403 靶基因 ppr PCNT115 AGO2 激素响应 体胚发生
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