Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male st...Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding.Despite its importance,there is a lack of research on the regulation mechanism of male reproductive development in watermelon.In this study,we identified that ClESR2,a VIIIb subclass member in the APETALA2/Ethylene Responsive Factor(AP2/ERF)superfamily,was a key factor in pollen development.RNA insitu hybridization confirmed significantClESR2 expression in the tapetum and pollen during the later stage of anther development.The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage.The RNA-seq and protein interaction assay confirmed that ClESR2 regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels.These suggest that Enhancer of Shoot Regeneration 2(ESR2)plays an important role in pollen maturation and vitality.This study helps understand the male reproductive development of watermelon,providing a theoretical foundation for developing male sterile materials.展开更多
Wuren walnut is a unique germplasm resource of Juglans sigillata in Guizhou Province,named after its purple and black seed coat.It is a typical medicinal and edible plant with high nutritional value and good taste com...Wuren walnut is a unique germplasm resource of Juglans sigillata in Guizhou Province,named after its purple and black seed coat.It is a typical medicinal and edible plant with high nutritional value and good taste compared with ordinary walnut.The color of its inner seed coat is different from that of ordinary walnut,which is mostly light yellow.Wuren walnut has great market potential for development.To lay a theoretical foundation for the future breeding of improved varieties,pollination tree configuration,and hybrid breeding of Wuren walnut in Guizhou Province,this paper summarized the current research status of pollen vitality and stigma acceptability of Guizhou Wuren walnut in recent years,and looked forward to future research and application.展开更多
After the treatment of pollen of larix olgensis at the different low temperature, the pollen vitality was observed by using fluorescent staining method. Several conclusions were obtained: 1) 0℃-3℃ is the best temper...After the treatment of pollen of larix olgensis at the different low temperature, the pollen vitality was observed by using fluorescent staining method. Several conclusions were obtained: 1) 0℃-3℃ is the best temperature range for storing pollen of Larix olgensis. 2) At the same temperature, humidity is the key factor influencing the pollen vitality. 3) Different clones shows different resistance to low temperature. Among the ten clones 2# clone shows the highest resistance.展开更多
The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The result...The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The results indicated that the pollen vi-tality (81.5%) of Syringa villosa was the highest among the seven tested species, followed by S. microphylla and S. meyeri, and that of S. oblata var. affinis was the lowest. The low temperature was the best condition for storage of pollen of Syringa, and the most proper temperature for the storage was 0-2 癈. The storability of S. microphylla was the best of all, and it could be stored over 60 days at the temperature of 0-2 癈, next was S. villosa and S. meyeri.展开更多
Peony pollen is a cheap and readily available biomass material with a relatively high protein content.In this work,it was employed as an N-rich precursor to prepare the nitrogen-doped porous carbon for supercapacitor ...Peony pollen is a cheap and readily available biomass material with a relatively high protein content.In this work,it was employed as an N-rich precursor to prepare the nitrogen-doped porous carbon for supercapacitor application.The porous carbon microspheres were prepared through a hydrothermal method and subsequent carbonization process.Notably,ammonium borofruoride and potassium hydroxide were employed respectively as an etchant and an activator to modify the porosity of the materials.The as prepared ANPPCs-700 has a super high BET specific surface area of 824.69 m^2/g.The microstructure,chemical state and electrochemical properties of the product were investigated in detail.The prepared nitrogen-doped carbon microspheres exhibits excellent specific capacity of 209 F/g at a current density of lA/g and remained 92.5%of the initial capacitance after 5000 deep cycles at 5 A/g.展开更多
Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China.Recent agricultural land expansion,however,not only causes its dwindling population size,but also poses a severe thr...Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China.Recent agricultural land expansion,however,not only causes its dwindling population size,but also poses a severe threat to its long-term persistence.Since our knowledge of the reproductive biology of P.delavayi is very limited,and some management misconceptions have further exacerbated the already worrisome situation,the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of P.delavayi,and to help correct some management misconceptions.Specifically,morphological traits of P.delavayi populations growing in two different habitats(i.e.,the open area versus forest understory),including basal stem diameter,plant height and flower numbers per plant,were measured and compared with each other.A range of floral manipulation experiments was further conducted to study the extent of self-compatibility and pollen limitation.The results showed that P.delavayi preferred the open habitat over forest understory as it generally grew bigger with thickerstems,and produced a higher number of flowers in the former habitat.Therefore,the open habitat should receive immediate priority for conservation action instead of being converted to agricultural land;also,P.delavayi is pollen limited,as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments(e.g.,non-supplemental self pollen,non-supplemental non-self pollen and supplemental self pollen),which is consistent with reported observations that alpine plants are more likely to be pollen-limited than lowland plants.We suggest that human intervention might be necessary to guarantee the long-term persistence of P.delavayi as harsh alpine environment,intense competition for pollinators and different anthropogenic perturbations co-limit its reproductive success.展开更多
研究不同性型蓖麻(Ricinus communis L.)花粉的育性,尤其是雌性系的花粉育性,对于蓖麻杂交育种工作意义重大。本研究首先利用TTC染色法和花粉萌发法分别检测了镶嵌型雌性系、两性系共6种蓖麻种质材料花粉的生活力,随后通过扫描电子显微...研究不同性型蓖麻(Ricinus communis L.)花粉的育性,尤其是雌性系的花粉育性,对于蓖麻杂交育种工作意义重大。本研究首先利用TTC染色法和花粉萌发法分别检测了镶嵌型雌性系、两性系共6种蓖麻种质材料花粉的生活力,随后通过扫描电子显微镜观察了他们的花粉形态,并利用SPSS软件分析了其形态特征值。结果显示:TTC染色和花粉萌发结果基本一致,不同材料间花粉生活力差异显著,蓖麻镶嵌型雌性系HCH3花粉生活力最大(96.97%)、镶嵌型雌性系HCH1花粉生活力次之。两种性型的花粉均呈现为单粒,长球形,赤道面观为长球形,极面观为三裂近圆形,中等大小(花粉极轴长为30.17~42.07μm),属于N_(3)P_(4)C_(3)型花粉。花粉外壁为网状纹饰,脊顶部具有密集的瘤状突起。然而,不同材料间花粉的极轴长、网眼直径、网脊宽度、单位面积网眼数形态值上差异显著。主成分析和聚类分析将6份蓖麻材料按花粉大小分为两类。本研究为蓖麻的花粉可育性和杂交选育提供了新的数据支撑。展开更多
基金support from the National Key Research and Development Program of China(2022YFD1602000)the National Natural Science Foundation of China(32202514,U22A20498 and 32072596)+2 种基金the Joint Fund of Henan Province Science and Technology Research and Development Plan,China(222103810009)the Science and Technology Innovation Team of Shaanxi,China(2021TD-32)the China Postdoctoral Science Foundation(2022M711064 and 2023M741062).
文摘Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding.Despite its importance,there is a lack of research on the regulation mechanism of male reproductive development in watermelon.In this study,we identified that ClESR2,a VIIIb subclass member in the APETALA2/Ethylene Responsive Factor(AP2/ERF)superfamily,was a key factor in pollen development.RNA insitu hybridization confirmed significantClESR2 expression in the tapetum and pollen during the later stage of anther development.The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage.The RNA-seq and protein interaction assay confirmed that ClESR2 regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels.These suggest that Enhancer of Shoot Regeneration 2(ESR2)plays an important role in pollen maturation and vitality.This study helps understand the male reproductive development of watermelon,providing a theoretical foundation for developing male sterile materials.
基金Supported by Guizhou Provincial Forestry Bureau Outstanding Youth Talent Training Special Fund Project(QLKH JZ[2022]05)National Natural Science(31860215)Guizhou Provincial Science and Technology Achievement Promotion Program(QKHCG[2020]1Y051)。
文摘Wuren walnut is a unique germplasm resource of Juglans sigillata in Guizhou Province,named after its purple and black seed coat.It is a typical medicinal and edible plant with high nutritional value and good taste compared with ordinary walnut.The color of its inner seed coat is different from that of ordinary walnut,which is mostly light yellow.Wuren walnut has great market potential for development.To lay a theoretical foundation for the future breeding of improved varieties,pollination tree configuration,and hybrid breeding of Wuren walnut in Guizhou Province,this paper summarized the current research status of pollen vitality and stigma acceptability of Guizhou Wuren walnut in recent years,and looked forward to future research and application.
文摘After the treatment of pollen of larix olgensis at the different low temperature, the pollen vitality was observed by using fluorescent staining method. Several conclusions were obtained: 1) 0℃-3℃ is the best temperature range for storing pollen of Larix olgensis. 2) At the same temperature, humidity is the key factor influencing the pollen vitality. 3) Different clones shows different resistance to low temperature. Among the ten clones 2# clone shows the highest resistance.
基金This research is supported by the NKBRSF (G1999043407-1) Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-406 & SCXZD0101).
文摘The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The results indicated that the pollen vi-tality (81.5%) of Syringa villosa was the highest among the seven tested species, followed by S. microphylla and S. meyeri, and that of S. oblata var. affinis was the lowest. The low temperature was the best condition for storage of pollen of Syringa, and the most proper temperature for the storage was 0-2 癈. The storability of S. microphylla was the best of all, and it could be stored over 60 days at the temperature of 0-2 癈, next was S. villosa and S. meyeri.
基金financial supports from the National Key Research and Development Program of China(Nos.2017YFB0102200,2017YFB0102900)。
文摘Peony pollen is a cheap and readily available biomass material with a relatively high protein content.In this work,it was employed as an N-rich precursor to prepare the nitrogen-doped porous carbon for supercapacitor application.The porous carbon microspheres were prepared through a hydrothermal method and subsequent carbonization process.Notably,ammonium borofruoride and potassium hydroxide were employed respectively as an etchant and an activator to modify the porosity of the materials.The as prepared ANPPCs-700 has a super high BET specific surface area of 824.69 m^2/g.The microstructure,chemical state and electrochemical properties of the product were investigated in detail.The prepared nitrogen-doped carbon microspheres exhibits excellent specific capacity of 209 F/g at a current density of lA/g and remained 92.5%of the initial capacitance after 5000 deep cycles at 5 A/g.
基金supported by China Special Fund for Forest Scientific Research in the Public Welfare (201204110)the National Natural Science Foundation of China (31460158)the Middle Aged Academic and Technical Leader Project of Yunnan Province (2010CI016)
文摘Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China.Recent agricultural land expansion,however,not only causes its dwindling population size,but also poses a severe threat to its long-term persistence.Since our knowledge of the reproductive biology of P.delavayi is very limited,and some management misconceptions have further exacerbated the already worrisome situation,the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of P.delavayi,and to help correct some management misconceptions.Specifically,morphological traits of P.delavayi populations growing in two different habitats(i.e.,the open area versus forest understory),including basal stem diameter,plant height and flower numbers per plant,were measured and compared with each other.A range of floral manipulation experiments was further conducted to study the extent of self-compatibility and pollen limitation.The results showed that P.delavayi preferred the open habitat over forest understory as it generally grew bigger with thickerstems,and produced a higher number of flowers in the former habitat.Therefore,the open habitat should receive immediate priority for conservation action instead of being converted to agricultural land;also,P.delavayi is pollen limited,as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments(e.g.,non-supplemental self pollen,non-supplemental non-self pollen and supplemental self pollen),which is consistent with reported observations that alpine plants are more likely to be pollen-limited than lowland plants.We suggest that human intervention might be necessary to guarantee the long-term persistence of P.delavayi as harsh alpine environment,intense competition for pollinators and different anthropogenic perturbations co-limit its reproductive success.
文摘研究不同性型蓖麻(Ricinus communis L.)花粉的育性,尤其是雌性系的花粉育性,对于蓖麻杂交育种工作意义重大。本研究首先利用TTC染色法和花粉萌发法分别检测了镶嵌型雌性系、两性系共6种蓖麻种质材料花粉的生活力,随后通过扫描电子显微镜观察了他们的花粉形态,并利用SPSS软件分析了其形态特征值。结果显示:TTC染色和花粉萌发结果基本一致,不同材料间花粉生活力差异显著,蓖麻镶嵌型雌性系HCH3花粉生活力最大(96.97%)、镶嵌型雌性系HCH1花粉生活力次之。两种性型的花粉均呈现为单粒,长球形,赤道面观为长球形,极面观为三裂近圆形,中等大小(花粉极轴长为30.17~42.07μm),属于N_(3)P_(4)C_(3)型花粉。花粉外壁为网状纹饰,脊顶部具有密集的瘤状突起。然而,不同材料间花粉的极轴长、网眼直径、网脊宽度、单位面积网眼数形态值上差异显著。主成分析和聚类分析将6份蓖麻材料按花粉大小分为两类。本研究为蓖麻的花粉可育性和杂交选育提供了新的数据支撑。
