This research aimed to improve selection of pepper seeds for separating high-quality seeds from low-quality seeds. Past research has shown that seed vigor is significantly related to the seed color and size, thus seve...This research aimed to improve selection of pepper seeds for separating high-quality seeds from low-quality seeds. Past research has shown that seed vigor is significantly related to the seed color and size, thus several physical features were identified as candidate predictors of high seed quality. Image recognition software was used to automate recognition of seed feature quality using 400 kernels of pepper cultivar 101. In addition, binary logistic regression and a neural network were applied to determine models with high predictive value of seed germination. Single-kernel germination tests were conducted to validate the predictive value of the identified features. The best predictors of seed vigor were determined by the highest correlation observed between the physical features and the subsequent fresh weight of seedlings that germinated from the 400 seeds. Correlation analysis showed that fresh weight was significantly positively correlated with eight physical features: three color features (R, a*, brightness), width, length, projected area, and single-kernel density, and weight. In contrast, fresh weight significantly negatively correlated with the feature of hue. In analyses of two of the highest correlating single features,' germination percentage increased from 59.3 to 71.8% when a*〉3, and selection rate peaked at 57.8%. Germination percentage increased from 59.3 to 79.4%, and the selection rate reached 76.8%, when single-kernel weight 〉0.0064 g. The most effective model was based on a multilayer perceptron (MLP) neural network, consisting of 15 physical traits as variables, and a stability calculated as 99.4%. Germination percentage in a calibration set of seeds was 79.1% and the selection rate was 90.0%. These results indicated that the model was effective in predicting seed germination based on physical features and could be used as a guide for quality control in seed selection. Automated systems based on machine vision and model classifiers can contribute to reducing the costs and labor required in the selection of pepper seeds.展开更多
A mathematical model for extraction of red pepper seed oil with supercritical CO2was proposed.Some factors influencing the process were investigated,including operation pressure,temperature and extraction yield Xe(%)....A mathematical model for extraction of red pepper seed oil with supercritical CO2was proposed.Some factors influencing the process were investigated,including operation pressure,temperature and extraction yield Xe(%).The model was solved by the method of weighted residuals and used to simulate the process numerically.The kinetic equation is expressed as Xe16.8606exp(t/9.98177)16.95457 and the simulation results are in excellent agreement with the experimental data.The optimal operating parameters are 30 MPa,318 K and 60 min.展开更多
Pepper is widely planted and used all over the world as fresh vegetable and spice.Genetic and morphological information of pepper are stored through seeds.Determination of seed variety is crucial for correctly identif...Pepper is widely planted and used all over the world as fresh vegetable and spice.Genetic and morphological information of pepper are stored through seeds.Determination of seed variety is crucial for correctly identifying genetic materials.Pepper varieties cannot be easily classified even by an expert eye due to the very small size of seeds and visual similarities.Hence,more advanced technologies are required to determine the variety of a pepper seed.A classification method was proposed to discriminate pepper seed based on neural networks and computer vision.Image acquisition was conducted using an office scanner at a resolution of 1200 dpi.Image features representing color,shape,and texture were extracted and used to classify pepper seeds.By calculating features from different color components,a feature database was constructed.Effective features were selected using sequential feature selection with different criterion functions.As a result of the feature selection procedure,the number of the features was significantly reduced from 257 to 10.Cross validation rules were applied to obtain a reliable classification model by preventing overfitting.Different numbers of neurons in the hidden layer and various training algorithms were investigated to determine the best multilayer perceptron model.The best classification performance was obtained using 30 neurons in the hidden layer of the network.With this network,an accuracy rate of 84.94%was achieved using the sequential feature selection and the training algorithm of resilient back propagation in classifying eight pepper seed varieties.展开更多
Objective The aim of this study was to explore the method and standard for rapidly screening low temperature-resistant pepper germplasm resources and provide a theoretical basis for the breeding of low temperature-res...Objective The aim of this study was to explore the method and standard for rapidly screening low temperature-resistant pepper germplasm resources and provide a theoretical basis for the breeding of low temperature-resistant pepper. [ Method ] With 110 pigment pepper seeds as the materials, their germination vigor under optimum temperature and suboptimal temperature were determined by means of roll rapid germination, and seeds with different genetic types were evaluated from aspects of germination vigor and its interval division. [ Result ] 37 pepper seeds with stronger low temperature resistance were screened. [ Conclusion]This study provides an important basis for screening low temperature-resistant pepper germplasm resources.展开更多
【目的】为研究γ-氨基丁酸(GABA)种子引发处理对盐胁迫下辣椒种子萌发和幼苗生长的效果及其可能机制。【方法】以茂蔬360朝天椒为材料,通过不同浓度γ-氨基丁酸(0、1.0、2.0、4.0、6.0、8.0μmol/L)种子引发处理,分析了100 mM NaCl模...【目的】为研究γ-氨基丁酸(GABA)种子引发处理对盐胁迫下辣椒种子萌发和幼苗生长的效果及其可能机制。【方法】以茂蔬360朝天椒为材料,通过不同浓度γ-氨基丁酸(0、1.0、2.0、4.0、6.0、8.0μmol/L)种子引发处理,分析了100 mM NaCl模拟盐胁迫下的辣椒种子萌发及幼苗生长的形态、生理和生化指标,【结果】浓度为6.0μmol/L的GABA引发处理显著增加了辣椒种子的可溶性糖、可溶性蛋白含量,降低了O_(2)-·和MDA的积累,提高了CAT酶活性、ASA及DHA含量;GABA引发处理提高了辣椒种子盐胁迫下发芽率22.6%、发芽势9.91倍、发芽指数56.3%及活力指数70.6%;GABA引发处理增加了盐胁迫下辣椒幼苗的地上部鲜质量、根系鲜质量、干质量及株高,降低了幼苗MDA含量、可溶性糖和脯氨酸含量,增加了SOD、CAT和APX活性。【结论】GABA通过提前启动种子贮藏物质的代谢及调控抗氧化防御能力增强辣椒种子萌发能力及耐盐性,并且在幼苗阶段再遇盐碱胁迫下,调控渗透调节物质含量和增强抗氧化酶活性,增加幼苗对盐胁迫的耐受性。展开更多
基金supported by the Beijing Municipal Science and Technology Project,China (Z151100001015004)
文摘This research aimed to improve selection of pepper seeds for separating high-quality seeds from low-quality seeds. Past research has shown that seed vigor is significantly related to the seed color and size, thus several physical features were identified as candidate predictors of high seed quality. Image recognition software was used to automate recognition of seed feature quality using 400 kernels of pepper cultivar 101. In addition, binary logistic regression and a neural network were applied to determine models with high predictive value of seed germination. Single-kernel germination tests were conducted to validate the predictive value of the identified features. The best predictors of seed vigor were determined by the highest correlation observed between the physical features and the subsequent fresh weight of seedlings that germinated from the 400 seeds. Correlation analysis showed that fresh weight was significantly positively correlated with eight physical features: three color features (R, a*, brightness), width, length, projected area, and single-kernel density, and weight. In contrast, fresh weight significantly negatively correlated with the feature of hue. In analyses of two of the highest correlating single features,' germination percentage increased from 59.3 to 71.8% when a*〉3, and selection rate peaked at 57.8%. Germination percentage increased from 59.3 to 79.4%, and the selection rate reached 76.8%, when single-kernel weight 〉0.0064 g. The most effective model was based on a multilayer perceptron (MLP) neural network, consisting of 15 physical traits as variables, and a stability calculated as 99.4%. Germination percentage in a calibration set of seeds was 79.1% and the selection rate was 90.0%. These results indicated that the model was effective in predicting seed germination based on physical features and could be used as a guide for quality control in seed selection. Automated systems based on machine vision and model classifiers can contribute to reducing the costs and labor required in the selection of pepper seeds.
基金Supported by the Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China(ZS12015)the Ministry of Education and Natural Science Foundation of Education Committee of Shaanxi Province(11JK0598)the 2012 Xi’an Modern Agricultural Promotion Project(NC1207)
文摘A mathematical model for extraction of red pepper seed oil with supercritical CO2was proposed.Some factors influencing the process were investigated,including operation pressure,temperature and extraction yield Xe(%).The model was solved by the method of weighted residuals and used to simulate the process numerically.The kinetic equation is expressed as Xe16.8606exp(t/9.98177)16.95457 and the simulation results are in excellent agreement with the experimental data.The optimal operating parameters are 30 MPa,318 K and 60 min.
文摘Pepper is widely planted and used all over the world as fresh vegetable and spice.Genetic and morphological information of pepper are stored through seeds.Determination of seed variety is crucial for correctly identifying genetic materials.Pepper varieties cannot be easily classified even by an expert eye due to the very small size of seeds and visual similarities.Hence,more advanced technologies are required to determine the variety of a pepper seed.A classification method was proposed to discriminate pepper seed based on neural networks and computer vision.Image acquisition was conducted using an office scanner at a resolution of 1200 dpi.Image features representing color,shape,and texture were extracted and used to classify pepper seeds.By calculating features from different color components,a feature database was constructed.Effective features were selected using sequential feature selection with different criterion functions.As a result of the feature selection procedure,the number of the features was significantly reduced from 257 to 10.Cross validation rules were applied to obtain a reliable classification model by preventing overfitting.Different numbers of neurons in the hidden layer and various training algorithms were investigated to determine the best multilayer perceptron model.The best classification performance was obtained using 30 neurons in the hidden layer of the network.With this network,an accuracy rate of 84.94%was achieved using the sequential feature selection and the training algorithm of resilient back propagation in classifying eight pepper seed varieties.
基金Supported by National Science and Technology Support Program"Key Technology for male-sterile breeding of main vegetable cropsintegration and seed industrialization"(2008BADB1B04)SeedProject of Vegetable Germplasm and Breeding in Shandong Province"Start-up Funding of High-level Talents in Qingdao Agricultureuniversity"(630912)~~
文摘Objective The aim of this study was to explore the method and standard for rapidly screening low temperature-resistant pepper germplasm resources and provide a theoretical basis for the breeding of low temperature-resistant pepper. [ Method ] With 110 pigment pepper seeds as the materials, their germination vigor under optimum temperature and suboptimal temperature were determined by means of roll rapid germination, and seeds with different genetic types were evaluated from aspects of germination vigor and its interval division. [ Result ] 37 pepper seeds with stronger low temperature resistance were screened. [ Conclusion]This study provides an important basis for screening low temperature-resistant pepper germplasm resources.
文摘【目的】为研究γ-氨基丁酸(GABA)种子引发处理对盐胁迫下辣椒种子萌发和幼苗生长的效果及其可能机制。【方法】以茂蔬360朝天椒为材料,通过不同浓度γ-氨基丁酸(0、1.0、2.0、4.0、6.0、8.0μmol/L)种子引发处理,分析了100 mM NaCl模拟盐胁迫下的辣椒种子萌发及幼苗生长的形态、生理和生化指标,【结果】浓度为6.0μmol/L的GABA引发处理显著增加了辣椒种子的可溶性糖、可溶性蛋白含量,降低了O_(2)-·和MDA的积累,提高了CAT酶活性、ASA及DHA含量;GABA引发处理提高了辣椒种子盐胁迫下发芽率22.6%、发芽势9.91倍、发芽指数56.3%及活力指数70.6%;GABA引发处理增加了盐胁迫下辣椒幼苗的地上部鲜质量、根系鲜质量、干质量及株高,降低了幼苗MDA含量、可溶性糖和脯氨酸含量,增加了SOD、CAT和APX活性。【结论】GABA通过提前启动种子贮藏物质的代谢及调控抗氧化防御能力增强辣椒种子萌发能力及耐盐性,并且在幼苗阶段再遇盐碱胁迫下,调控渗透调节物质含量和增强抗氧化酶活性,增加幼苗对盐胁迫的耐受性。