[Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides（Mart.）Griseb.[Method] Effects of fragmentation intensity of fresh roots and their bu...[Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides（Mart.）Griseb.[Method] Effects of fragmentation intensity of fresh roots and their burial depth on sprouting and early growth of A.philoxeroides were studied by control test.[Result] More sprouts of A.philoxeroides emerged when the fragmentation intensity of fresh roots was higher,while if the fragmentation intensity of fresh roots was lower,the early growth of A.philoxeroides was more rapid.The soil buried depth had significant effect on fresh root sprouts＇ emergence,but once fresh root sprouts could reach the soil surface and were given enough growth time,even if the fresh roots were buried in different depths,soil buried depth had no significant effect on its young plant growth.[Conclusion] If different fragmentation intensities of fresh roots present,there is a kind of trade-off strategy between root sprouts＇ emergence and plant＇ early growth,by which A.philoxeroides can invade new habitat successfully.To control the invasion of A.philoxeroides,it is critical to prevent its fresh root sprouts from emerging to soil surface,that is,to bury the fresh roots at a further soil depth.展开更多
[Objective] This study aimed to establish an effective detection technology for rapidly identifying Bt transgenic sugarcane, promptly removing non-Bt transgenic plants and increasing breeding efficiency of Bt transgen...[Objective] This study aimed to establish an effective detection technology for rapidly identifying Bt transgenic sugarcane, promptly removing non-Bt transgenic plants and increasing breeding efficiency of Bt transgenic plants, thereby improving breeding efficiency of insect-resistant sugarcane cultivars in China. [ Method] Approximately 1 mg of root tips and other materials were crushed, placed in the bottom of PCR tubes, added successively with solution I and mineral oil, headed at 95 ℃ for 15 rain, added with solution Ⅱ, and finally added with solution III containing GBt primer for PCR. After separated by using electrophoresis on agarese gel, amplification products were observed and photographed under a gel imaging system. [ Result] Various materials treated with different concentrations of solution led to different amplification results. Specifically, the appropriate concentration of NaOH in solution I was 0.1 -0.2 mol/L; the appropriate pH value of solution II was 2. No bands were amplified from mature leaves, tender leaves and old roots of Bt transgenic sugarcane, while root tips successfully amplified 545 bp target bands. The established method was used to identify and analyze 63 hybrid seedlings of YT 91 -976 × SBR216, results showed that a total of 17 samples exhibited 545 bp bands, accounting for approximately 27% of the total number of hybrid seedlings, indicating that these 17 seedlings were Bt transgenic sugarcane plants. [ Conclusion] The rapid detection method established in this study is conducive to identifying Bt transgenic sugarcane and improving breeding efficiency of insect-resistant sugarcane lines, which provides basis for screening of other transgenic materials.展开更多
The impacts of pH on roots growth of sugarcane seedlings were explored by a pot test. The nutrient solution with three pH levels, Le., 5.0, 7.0 and 8.5 were applied to culture five combination seedlings, including A ...The impacts of pH on roots growth of sugarcane seedlings were explored by a pot test. The nutrient solution with three pH levels, Le., 5.0, 7.0 and 8.5 were applied to culture five combination seedlings, including A （RB85-5156 × YR05-407）, B （YR06-3501 ×Tolodo）, C （YT94-128 × GZ75-65）, D （ROC6 × YR05-733） and E （VMC87-95 ×YR05-171）. The result indicated that root length, root surface, root volume and root diameter were all in the largest in B combination with solution pH= 7.0, and the root length, root surface, root volume were in the largest in combination A, C, D and E with solution pH=8.5. Root activity in combination C, D and E were in the highest with solution pH=5.0, while it was in the highest for combination B when pH=7.0. In general, the root growth and morphology in all five combinations were better in the solutions with pH of 7.0 and 8.5.展开更多
Background:Standing root biomass stocks are larger in the perennial grain intermediate wheatgrass(IWG;Thinopyrum intermedium[Host]Barkworth and Dewey)than annual spring wheat(Triticum aestivum L.).However,previous stu...Background:Standing root biomass stocks are larger in the perennial grain intermediate wheatgrass(IWG;Thinopyrum intermedium[Host]Barkworth and Dewey)than annual spring wheat(Triticum aestivum L.).However,previous studies have not separated root growth from root decomposition,which presents a significant gap in our understanding of how roots can contribute to soil organic carbon(C)accrual or other soil properties through time.Methods:We used paired sequential coring and root ingrowth cores to measure standing root stock,new root production,root decomposition,and decomposed root C and N from 0 to 15 cm soil depth of 1-year-old IWG(IWG-1),2-year-old IWG(IWG-2),and annual spring wheat.Results:Standing root stock was 3.2–6.5 and 6.3–9.9 times higher in IWG-1 and IWG-2 than wheat.Total root production was 1.7 times greater in IWG-1 than IWG-2.Conversely,root decomposition almost doubled from 1.39 to 2.43 kg m-3 between IWG-1 and IWG-2.Conclusions:In IWG,decreased root production and increased root decomposi-tion with stand age suggest a change in growth strategy that could reduce the contribution of root-derived C to stabilized soil C pools as IWG stands age.展开更多
基金Supported by Program from Hubei Education Department(Z200512002)Outstanding Youth Science and Technology Innovation Team Plan Project of Yangtze University~~
文摘[Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides（Mart.）Griseb.[Method] Effects of fragmentation intensity of fresh roots and their burial depth on sprouting and early growth of A.philoxeroides were studied by control test.[Result] More sprouts of A.philoxeroides emerged when the fragmentation intensity of fresh roots was higher,while if the fragmentation intensity of fresh roots was lower,the early growth of A.philoxeroides was more rapid.The soil buried depth had significant effect on fresh root sprouts＇ emergence,but once fresh root sprouts could reach the soil surface and were given enough growth time,even if the fresh roots were buried in different depths,soil buried depth had no significant effect on its young plant growth.[Conclusion] If different fragmentation intensities of fresh roots present,there is a kind of trade-off strategy between root sprouts＇ emergence and plant＇ early growth,by which A.philoxeroides can invade new habitat successfully.To control the invasion of A.philoxeroides,it is critical to prevent its fresh root sprouts from emerging to soil surface,that is,to bury the fresh roots at a further soil depth.
基金Supported by Modern Agricultural Industry Technology System (CARS-2004B)
文摘[Objective] This study aimed to establish an effective detection technology for rapidly identifying Bt transgenic sugarcane, promptly removing non-Bt transgenic plants and increasing breeding efficiency of Bt transgenic plants, thereby improving breeding efficiency of insect-resistant sugarcane cultivars in China. [ Method] Approximately 1 mg of root tips and other materials were crushed, placed in the bottom of PCR tubes, added successively with solution I and mineral oil, headed at 95 ℃ for 15 rain, added with solution Ⅱ, and finally added with solution III containing GBt primer for PCR. After separated by using electrophoresis on agarese gel, amplification products were observed and photographed under a gel imaging system. [ Result] Various materials treated with different concentrations of solution led to different amplification results. Specifically, the appropriate concentration of NaOH in solution I was 0.1 -0.2 mol/L; the appropriate pH value of solution II was 2. No bands were amplified from mature leaves, tender leaves and old roots of Bt transgenic sugarcane, while root tips successfully amplified 545 bp target bands. The established method was used to identify and analyze 63 hybrid seedlings of YT 91 -976 × SBR216, results showed that a total of 17 samples exhibited 545 bp bands, accounting for approximately 27% of the total number of hybrid seedlings, indicating that these 17 seedlings were Bt transgenic sugarcane plants. [ Conclusion] The rapid detection method established in this study is conducive to identifying Bt transgenic sugarcane and improving breeding efficiency of insect-resistant sugarcane lines, which provides basis for screening of other transgenic materials.
基金Supported by National Modern Agriculture Industry Technology System Construction Project(CARS-20-1-1)Yunnan Key New Product Development and Planning Project(2011BB005)Yunnan Key New Product Development and Planning(2012BB014)~~
文摘The impacts of pH on roots growth of sugarcane seedlings were explored by a pot test. The nutrient solution with three pH levels, Le., 5.0, 7.0 and 8.5 were applied to culture five combination seedlings, including A （RB85-5156 × YR05-407）, B （YR06-3501 ×Tolodo）, C （YT94-128 × GZ75-65）, D （ROC6 × YR05-733） and E （VMC87-95 ×YR05-171）. The result indicated that root length, root surface, root volume and root diameter were all in the largest in B combination with solution pH= 7.0, and the root length, root surface, root volume were in the largest in combination A, C, D and E with solution pH=8.5. Root activity in combination C, D and E were in the highest with solution pH=5.0, while it was in the highest for combination B when pH=7.0. In general, the root growth and morphology in all five combinations were better in the solutions with pH of 7.0 and 8.5.
文摘Background:Standing root biomass stocks are larger in the perennial grain intermediate wheatgrass(IWG;Thinopyrum intermedium[Host]Barkworth and Dewey)than annual spring wheat(Triticum aestivum L.).However,previous studies have not separated root growth from root decomposition,which presents a significant gap in our understanding of how roots can contribute to soil organic carbon(C)accrual or other soil properties through time.Methods:We used paired sequential coring and root ingrowth cores to measure standing root stock,new root production,root decomposition,and decomposed root C and N from 0 to 15 cm soil depth of 1-year-old IWG(IWG-1),2-year-old IWG(IWG-2),and annual spring wheat.Results:Standing root stock was 3.2–6.5 and 6.3–9.9 times higher in IWG-1 and IWG-2 than wheat.Total root production was 1.7 times greater in IWG-1 than IWG-2.Conversely,root decomposition almost doubled from 1.39 to 2.43 kg m-3 between IWG-1 and IWG-2.Conclusions:In IWG,decreased root production and increased root decomposi-tion with stand age suggest a change in growth strategy that could reduce the contribution of root-derived C to stabilized soil C pools as IWG stands age.
