Heavy metals,polycyclic aromatic hydrocarbons(PAHs),and perfluorinated alkyl substances(PFASs)polluted in 155 samples of two marine crustaceans(Oratosquilla oratoria and Portunus trituberculatus)harvested in the Zhous...Heavy metals,polycyclic aromatic hydrocarbons(PAHs),and perfluorinated alkyl substances(PFASs)polluted in 155 samples of two marine crustaceans(Oratosquilla oratoria and Portunus trituberculatus)harvested in the Zhoushan fishing ground in the East China Sea were assessed.The results showed that Pb,Hg,and iAs in the whole edible parts of O.oratoria and P.trituberculatus were at trace levels,far below the limits set by the China’s national standard,whereas the exceeding standard rates of Cd in the whole edible parts of O.oratoria and P.trituberculatus were 98.6%and 75.6%,respectively.Moreover,the average Cd levels and the exceeding standard rates of Cd in the visceral tissues of O.oratoria were significantly higher than those in the muscle tissues(4.64 mg kg^(−1)vs.0.49 mg kg^(−1)and 96.7%vs 30%,respectively).PAHs with high detection frequency in the two marine crustaceans included NA,AC,FL,and PHE.Total contents of 15 PAHs(abbreviated asΣ15 PAHs)in whole edible parts of O.oratoria and P.trituberculatus were 10.75–65.9μg kg^(−1)(mean=18.7μg kg^(−1)and 13.26–181μg kg^(−1)(mean=24.2μg kg^(−1),and those in the muscle and the visceral tissues of P.trituberculatus were 9.58–160μg kg^(−1)(mean=21.1μg kg^(−1)and 18.22–201μg kg^(−1)(mean=32.7μg kg^(−1),respectively.Furthermore,PFASs that were found at high contents included PFOA,PFOS,and PFOSA,and total contents of 23 PFASs(Σ23 PFASs)ranged 0.0162–5.104μg kg^(−1)(mean=0.915μg kg^(−1).Collectively,the present work provides new data on the contamination in two marine crustaceans captured in the Zhoushan fishing ground in the China East Sea,which can be useful for making suggestions on proper consumption amounts of the two marine crustaceans.展开更多
建立了高效液相色谱-串联质谱(HPLC-MS/MS)同时测定蜂蜜中20种全氟烷基化合物(PFASs)含量的分析方法。采用改进的QuEChERS方法对样品进行前处理,用含1.5%(v/v)甲酸的乙腈溶液振荡提取,C_(18)和N-丙基乙二胺(PSA)吸附剂净化,Atlantis T3 ...建立了高效液相色谱-串联质谱(HPLC-MS/MS)同时测定蜂蜜中20种全氟烷基化合物(PFASs)含量的分析方法。采用改进的QuEChERS方法对样品进行前处理,用含1.5%(v/v)甲酸的乙腈溶液振荡提取,C_(18)和N-丙基乙二胺(PSA)吸附剂净化,Atlantis T3 C_(18)色谱柱(150 mm×2.1 mm,3μm)分离,以含5 mmol/L乙酸铵的甲醇溶液和5 mmol/L乙酸铵溶液为流动相进行梯度洗脱。在电喷雾离子(ESI)源负离子模式下以多反应监测(MRM)扫描,采用同位素内标法进行定量分析。结果表明,20种PFASs在16 min内即可完成分离,在0.2~10μg/L范围内线性关系良好,相关系数(r)均大于0.99;检出限(LOD,S/N=3)和定量限(LOQ,S/N=10)分别为0.04~0.10μg/kg和0.10~0.20μg/kg。在0.1、0.5、1.0和2.0μg/kg加标水平下,20种PFASs的加标回收率为72.6%~113.0%,RSD为0.4%~15.9%(n=6)。该法快速、高效、准确,适用于蜂蜜样品中20种PFASs的同时检测。展开更多
基金the National Key R&D Program of China(No.2020YFD0900900)the Public Interest Science and Technology Plan of Ningbo(No.2019C10007)the Basic Public Welfare Project of Zhejiang Provincial Department of Science and Technol ogy(No.LGN20C200015).
文摘Heavy metals,polycyclic aromatic hydrocarbons(PAHs),and perfluorinated alkyl substances(PFASs)polluted in 155 samples of two marine crustaceans(Oratosquilla oratoria and Portunus trituberculatus)harvested in the Zhoushan fishing ground in the East China Sea were assessed.The results showed that Pb,Hg,and iAs in the whole edible parts of O.oratoria and P.trituberculatus were at trace levels,far below the limits set by the China’s national standard,whereas the exceeding standard rates of Cd in the whole edible parts of O.oratoria and P.trituberculatus were 98.6%and 75.6%,respectively.Moreover,the average Cd levels and the exceeding standard rates of Cd in the visceral tissues of O.oratoria were significantly higher than those in the muscle tissues(4.64 mg kg^(−1)vs.0.49 mg kg^(−1)and 96.7%vs 30%,respectively).PAHs with high detection frequency in the two marine crustaceans included NA,AC,FL,and PHE.Total contents of 15 PAHs(abbreviated asΣ15 PAHs)in whole edible parts of O.oratoria and P.trituberculatus were 10.75–65.9μg kg^(−1)(mean=18.7μg kg^(−1)and 13.26–181μg kg^(−1)(mean=24.2μg kg^(−1),and those in the muscle and the visceral tissues of P.trituberculatus were 9.58–160μg kg^(−1)(mean=21.1μg kg^(−1)and 18.22–201μg kg^(−1)(mean=32.7μg kg^(−1),respectively.Furthermore,PFASs that were found at high contents included PFOA,PFOS,and PFOSA,and total contents of 23 PFASs(Σ23 PFASs)ranged 0.0162–5.104μg kg^(−1)(mean=0.915μg kg^(−1).Collectively,the present work provides new data on the contamination in two marine crustaceans captured in the Zhoushan fishing ground in the China East Sea,which can be useful for making suggestions on proper consumption amounts of the two marine crustaceans.
文摘建立了使用固相萃取-液相色谱-串联质谱(SPE-LC-MS/MS)同时检测食品包装材料中16种全氟烷基类化合物(PFAS)的方法。分别对样品前处理方法、质谱条件等进行了比较和优化,样品用甲醇超声提取,经Oasis WAX固相萃取小柱净化后,用Atlantis T3 C18色谱柱分离,以乙腈和5 mmol/L乙酸铵溶液为流动相进行梯度洗脱,多反应监测(MRM)负离子模式扫描,同位素内标法和外标法结合定量。16种PFAS在0.5~20.0μg/L范围内线性关系良好,相关系数(r^2)均大于0.99。加标回收率为68.6%~109.2%,RSD为2.5%~18.1%(n=6)。检出限为0.2~0.5μg/kg,定量限为0.5~1.0μg/kg。该方法简便、快速、准确,可用于食品包装材料样品中PFAS的检测。
