Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroit...Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroitin sulfate)double network hydrogel was constructed in this paper.It was not only multiply bionic in its composition,structure and mechanical strength,but also endowed with the ability to immunomodulate microenvironment and simultaneously induce in situ repair of defected TMJ discs.On the one hand,it inhibited inflammatory effects of inflammasome in macrophages,reduced the extracellular matrix(ECM)-degrading enzymes secreted by chondrocytes,reversed the local inflammatory state,promoted the proliferation of TMJ disc cells and induced fibrochondrogenic differentiation of synovium-derived mesenchymal stem cells(SMSCs).On the other hand,it gave an impetus to repairing a relatively-large(6 mm-sized)defect in mini pigs’TMJ discs in a rapid and high-quality manner,which suggested a promising clinical application.展开更多
基金This work was sponsored by grants from the National Nature Science Foundation of China(82072070)the National Key Research and Development Program of China(2018YFC1105202)Science and Technology Commission of Shanghai Municipality(21DZ2291700,18DZ2290300).
文摘Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroitin sulfate)double network hydrogel was constructed in this paper.It was not only multiply bionic in its composition,structure and mechanical strength,but also endowed with the ability to immunomodulate microenvironment and simultaneously induce in situ repair of defected TMJ discs.On the one hand,it inhibited inflammatory effects of inflammasome in macrophages,reduced the extracellular matrix(ECM)-degrading enzymes secreted by chondrocytes,reversed the local inflammatory state,promoted the proliferation of TMJ disc cells and induced fibrochondrogenic differentiation of synovium-derived mesenchymal stem cells(SMSCs).On the other hand,it gave an impetus to repairing a relatively-large(6 mm-sized)defect in mini pigs’TMJ discs in a rapid and high-quality manner,which suggested a promising clinical application.
