Perilla seed is one of the traditional Chinese herbs normally used both as food and medicine for its various functional ingrediems such as protein, amino acids, ct-linolenic acid, flavones, rosmarinic acid, terpenes, ...Perilla seed is one of the traditional Chinese herbs normally used both as food and medicine for its various functional ingrediems such as protein, amino acids, ct-linolenic acid, flavones, rosmarinic acid, terpenes, polysaccharides, etc.. Pefilla seed is with physiological features in slowing down decrepitude, lower- ing blood pressure, cholesterol, inhibiting cancer cell differentiation, and improving body immune. The present paper summarized research progress at home and abroad on chemical component analysis, extraction technology and application status of perilla seed in the fields of feed, health care, food and chemical industry in recent years, in order to provide a reference basis for reasonable development of perilla seed.展开更多
Objective To optimize the extraction technology of perilla seeds oil from the oil cake of perilla seeds(OCPS)by using the contents of active fatty acids as evaluation standard.Methods The fatty acids were extracted fr...Objective To optimize the extraction technology of perilla seeds oil from the oil cake of perilla seeds(OCPS)by using the contents of active fatty acids as evaluation standard.Methods The fatty acids were extracted from OCPS,the residue of perilla seeds after cold-press,by smashing tissue extraction(STE),the new technology selected through comparing with classical leaching extraction(LE),Soxhlet extraction(SE),ultrasonic extraction(UE),and supercritical-CO2 fluid extraction(SFE).For optimized condition of STE,orthogonal test was designed and completed.The contents of five fatty acids in extracted oil and OCPS were determined by GC.Results The optimized extraction parameters were smashing for 1.5 min under extraction power of 150 W and 1:6 of the material/solvent ratio.The contents of five fatty acids in the oils extracted by five techniques from OCPS and determined by GC were as follows:α-linolenic acid(41.12%-51.81%),linoleic acid(15.38%-16.43%),oleic acid (18.93%-27.28%),stearic acid(2.56%-4.01%),and palmitic acid(7.38%-10.77%).Conclusion The results show that STE is the most efficient technology with the highest yield(LE:0.57%;SE:1.03%;UE:0.61%;SFE:0.80%;STE: 1.17%)and shortest time(LE:720 min;SE:360 min;UE:30 min;SFE:120 min;STE:1.5 min)among five tested extraction technologies.It is first reported using STE to extract herbal oil enriched with active fatty acids.展开更多
利用响应面分析法优化泡沫法提取紫苏蛋白工艺,为紫苏资源的开发利用提供理论依据与技术参数。在单因素试验基础上,设计浸泡温度、液料比和浸泡时间3个因素为自变量,紫苏蛋白提取率为响应值,采用Box-Behnken试验设计方法,研究各自变量...利用响应面分析法优化泡沫法提取紫苏蛋白工艺,为紫苏资源的开发利用提供理论依据与技术参数。在单因素试验基础上,设计浸泡温度、液料比和浸泡时间3个因素为自变量,紫苏蛋白提取率为响应值,采用Box-Behnken试验设计方法,研究各自变量及其交互作用对紫苏蛋白提取率的影响。利用Design Expert软件得到回归方程的预测模型并进行响应面分析,紫苏饼粕蛋白提取率对浸泡温度、液料比和浸泡时间二次方程模型为:Y=13.21–2.21A–0.21B–1.38C–0.15AB–2.43AC–1.78BC–3.37A2–4.43B2–1.99C2(R2=0.9544),该模型拟合程度较好,其中浸泡温度对紫苏蛋白提取率有极显著影响(P<0.01),浸泡时间有显著影响(P<0.05),浸泡温度和浸泡时间的交互作用对其有极显著影响,液料比和浸泡时间对其有显著影响。确定紫苏蛋白提取率的最佳条件为:浸泡温度53℃,液料比10:1 m L·g-1,浸泡时间114 min,在此最佳试验条件下,紫苏蛋白提取率为13.91%,与理论预测值相比,其相对误差约为2.10%。试验建立的由响应面设计优化紫苏蛋白泡沫法提取工艺模型拟合效果较好,可以用于实际预测,即该响应面试验设计对泡沫法分离提取紫苏蛋白工艺的优化结果是有效的。展开更多
以紫苏籽粕为原料,通过单因素和响应面试验对闪式提取和超声提取紫苏籽粕蛋白的工艺进行了优化,并研究了不同提取方式对紫苏籽粕蛋白功能性质的影响。结果表明,超声提取分离蛋白(ultrasonic extraction of protein isolate,UEOPI)的提...以紫苏籽粕为原料,通过单因素和响应面试验对闪式提取和超声提取紫苏籽粕蛋白的工艺进行了优化,并研究了不同提取方式对紫苏籽粕蛋白功能性质的影响。结果表明,超声提取分离蛋白(ultrasonic extraction of protein isolate,UEOPI)的提取率为53.85%,闪式提取分离蛋白(flash extraction of protein isolate,FEOPI)的提取率为46.54%,而UEOPI所需的时间为FEOPI所需时间的40倍。两种分离蛋白的分子量范围均为14.4~50 kDa,但UEOPI的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)图谱条带比FEOPI的条带多。UEOPI和FEOPI纯度分别为91.306%和93.923%,纯度均较高。UEOPI比FEOPI有更好的DPPH、ABTS、O_(2)^(-)、OH自由基清除能力和还原力;更高的溶解性(NSI)、持水性(WHC)、乳化性(EAI)、乳化稳定性(ES)、起泡性和起泡稳定性。展开更多
基金Supported by Programs for Science and Technology Development of Henan Province(132102310279)
文摘Perilla seed is one of the traditional Chinese herbs normally used both as food and medicine for its various functional ingrediems such as protein, amino acids, ct-linolenic acid, flavones, rosmarinic acid, terpenes, polysaccharides, etc.. Pefilla seed is with physiological features in slowing down decrepitude, lower- ing blood pressure, cholesterol, inhibiting cancer cell differentiation, and improving body immune. The present paper summarized research progress at home and abroad on chemical component analysis, extraction technology and application status of perilla seed in the fields of feed, health care, food and chemical industry in recent years, in order to provide a reference basis for reasonable development of perilla seed.
基金Chinese Northeast Characteristic Nutritional Plant Oil Construction Foundation and Industrialization Item(No.2008301026)
文摘Objective To optimize the extraction technology of perilla seeds oil from the oil cake of perilla seeds(OCPS)by using the contents of active fatty acids as evaluation standard.Methods The fatty acids were extracted from OCPS,the residue of perilla seeds after cold-press,by smashing tissue extraction(STE),the new technology selected through comparing with classical leaching extraction(LE),Soxhlet extraction(SE),ultrasonic extraction(UE),and supercritical-CO2 fluid extraction(SFE).For optimized condition of STE,orthogonal test was designed and completed.The contents of five fatty acids in extracted oil and OCPS were determined by GC.Results The optimized extraction parameters were smashing for 1.5 min under extraction power of 150 W and 1:6 of the material/solvent ratio.The contents of five fatty acids in the oils extracted by five techniques from OCPS and determined by GC were as follows:α-linolenic acid(41.12%-51.81%),linoleic acid(15.38%-16.43%),oleic acid (18.93%-27.28%),stearic acid(2.56%-4.01%),and palmitic acid(7.38%-10.77%).Conclusion The results show that STE is the most efficient technology with the highest yield(LE:0.57%;SE:1.03%;UE:0.61%;SFE:0.80%;STE: 1.17%)and shortest time(LE:720 min;SE:360 min;UE:30 min;SFE:120 min;STE:1.5 min)among five tested extraction technologies.It is first reported using STE to extract herbal oil enriched with active fatty acids.
文摘利用响应面分析法优化泡沫法提取紫苏蛋白工艺,为紫苏资源的开发利用提供理论依据与技术参数。在单因素试验基础上,设计浸泡温度、液料比和浸泡时间3个因素为自变量,紫苏蛋白提取率为响应值,采用Box-Behnken试验设计方法,研究各自变量及其交互作用对紫苏蛋白提取率的影响。利用Design Expert软件得到回归方程的预测模型并进行响应面分析,紫苏饼粕蛋白提取率对浸泡温度、液料比和浸泡时间二次方程模型为:Y=13.21–2.21A–0.21B–1.38C–0.15AB–2.43AC–1.78BC–3.37A2–4.43B2–1.99C2(R2=0.9544),该模型拟合程度较好,其中浸泡温度对紫苏蛋白提取率有极显著影响(P<0.01),浸泡时间有显著影响(P<0.05),浸泡温度和浸泡时间的交互作用对其有极显著影响,液料比和浸泡时间对其有显著影响。确定紫苏蛋白提取率的最佳条件为:浸泡温度53℃,液料比10:1 m L·g-1,浸泡时间114 min,在此最佳试验条件下,紫苏蛋白提取率为13.91%,与理论预测值相比,其相对误差约为2.10%。试验建立的由响应面设计优化紫苏蛋白泡沫法提取工艺模型拟合效果较好,可以用于实际预测,即该响应面试验设计对泡沫法分离提取紫苏蛋白工艺的优化结果是有效的。
文摘以紫苏籽粕为原料,通过单因素和响应面试验对闪式提取和超声提取紫苏籽粕蛋白的工艺进行了优化,并研究了不同提取方式对紫苏籽粕蛋白功能性质的影响。结果表明,超声提取分离蛋白(ultrasonic extraction of protein isolate,UEOPI)的提取率为53.85%,闪式提取分离蛋白(flash extraction of protein isolate,FEOPI)的提取率为46.54%,而UEOPI所需的时间为FEOPI所需时间的40倍。两种分离蛋白的分子量范围均为14.4~50 kDa,但UEOPI的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)图谱条带比FEOPI的条带多。UEOPI和FEOPI纯度分别为91.306%和93.923%,纯度均较高。UEOPI比FEOPI有更好的DPPH、ABTS、O_(2)^(-)、OH自由基清除能力和还原力;更高的溶解性(NSI)、持水性(WHC)、乳化性(EAI)、乳化稳定性(ES)、起泡性和起泡稳定性。
