Peripartal cows often experience negative energy balance,and are therefore prone to suffering from metabolic diseases such as hyperketonemia,which causes financial losses in dairy farms.This study aimed to investigate...Peripartal cows often experience negative energy balance,and are therefore prone to suffering from metabolic diseases such as hyperketonemia,which causes financial losses in dairy farms.This study aimed to investigate the effect of green tea polyphenol(GTP)supplementation during the periparturient period on production performance,oxidative stress and immunometabolism in dairy cows with hyperketonemia.One hundred Holstein cows were assigned to GTP(0.2 g/kg DM;n=50)or control(without GTP;n=50)group based on body weight,previous milk yield,and parity on d 15 before expected parturition.Subsequently,10 cows with hyperketonemia were selected from each group,according to bloodβ-hydroxybutyric acid(BHBA)concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum.All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum.Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10,20,and 30 d postpartum.Compared with control cows,greater milk yield and lower somatic cell count were observed in GTP cows.The GTP group had lower concentrations of BHBA,free fatty acids,cholesterol,triglyceride,reactive oxygen species,malondialdehyde,and hydrogen peroxide,greater concentrations of glucose,lower activities of aspartate aminotransferase,alanine aminotransferase,and glutamyl transpeptidase,alongside greater ac-tivities of superoxide dismutase,glutathione peroxidase,and total antioxidant capacity.Additionally,GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10,but down-regulated concentrations of tumor necrosis factor-α,interleukin-1β,interleukin-2,interleukin-8,and interferon-γin plasma.Greater concentrations of plasma immunoglobulin G were also detected in the GTP group.Overall,the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hvoerketonemia during early lactation.展开更多
基金grants from National Natural Science Foundation of China(no.32060765,31601975,31460616,Beijing,China)Inner Mongolia Natural Science Foundation of China(no.2019BS03033,Hohhot,China)+2 种基金Ningxia University Scientific Research Start-up Project,Inner Mongolia Academy of Agricultural&Animal Husbandry Science Innovation Foundation(no.2018QNJJM08,2019GXJJM06,Hohhot,China)Inner Mongolia Autonomous Region Foundation for Innovation Initiation Support Program for Overseas Students(Hohhot,China)Inner Mongolia Autonomous Region Ministry of Finance Agricultural and husbandry Science and Technology Promotion Project(no.2020TG07-6,Hohhot,China).
文摘Peripartal cows often experience negative energy balance,and are therefore prone to suffering from metabolic diseases such as hyperketonemia,which causes financial losses in dairy farms.This study aimed to investigate the effect of green tea polyphenol(GTP)supplementation during the periparturient period on production performance,oxidative stress and immunometabolism in dairy cows with hyperketonemia.One hundred Holstein cows were assigned to GTP(0.2 g/kg DM;n=50)or control(without GTP;n=50)group based on body weight,previous milk yield,and parity on d 15 before expected parturition.Subsequently,10 cows with hyperketonemia were selected from each group,according to bloodβ-hydroxybutyric acid(BHBA)concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum.All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum.Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10,20,and 30 d postpartum.Compared with control cows,greater milk yield and lower somatic cell count were observed in GTP cows.The GTP group had lower concentrations of BHBA,free fatty acids,cholesterol,triglyceride,reactive oxygen species,malondialdehyde,and hydrogen peroxide,greater concentrations of glucose,lower activities of aspartate aminotransferase,alanine aminotransferase,and glutamyl transpeptidase,alongside greater ac-tivities of superoxide dismutase,glutathione peroxidase,and total antioxidant capacity.Additionally,GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10,but down-regulated concentrations of tumor necrosis factor-α,interleukin-1β,interleukin-2,interleukin-8,and interferon-γin plasma.Greater concentrations of plasma immunoglobulin G were also detected in the GTP group.Overall,the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hvoerketonemia during early lactation.
