Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Me...Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Methods We compared the peritoneal macrophage activity obtained from the irrigation solution of theuremia patients (n=2) immediately after inserting the catheter and with those from the long - dwell time dialysate(≥10h) of peritoneal dialysis patients (n= 7). The capacity of peritoneal macrophage to release TNF - x was assayedby L929 cytotoxicity. At the same time, we compared TNF- 7 activity among different glucose concentration (1.5%,2.5% and 4.25% dextrose) of dialysate. Results The levels of TNF- a in CAPD and control group were 170 and517 pg/106 respectively (P<0.01). The patients who were using dialysate containing higher glucose concentrationhad lower TNF- x level (P<0.05, P<0.01). Conclusion It is suggested that the inhibitory effect of peritonealdialysate on peritoneal macrophages might continue at least 10h in vivo. The higher glucose concentration ofdialysate may have worse effect on peritoneal macrophage activity.展开更多
Background A multi-center large scale study is needed to confirm the efficacy and safety of domestic peritoneal dialysis （PD） solutions. Some researchers believe that 6 Lid is enough for adequate dialysis, but there...Background A multi-center large scale study is needed to confirm the efficacy and safety of domestic peritoneal dialysis （PD） solutions. Some researchers believe that 6 Lid is enough for adequate dialysis, but there is no multi-center prospective study on Chinese population to confirm this. In this study, we evaluated the efficacy and safety of domestic PD solution （Changfu） and its difference between 6 L and 8 L dosage. Methods Adult PD patients who had taken PD therapy for at least one month were selected and divided into four groups according to two dialysis solution brands and two dialysis dosages, i.e., 6 L dose with Changfu dialysis solution, 6 L dose with Baxter dialysis solution, 8 L dose with Changfu dialysis solution, and 8 L dose with Baxter dialysis solution. After 48 weeks, the changes of primary and secondary efficacy indices were compared between different types and different dosages. We also analyzed the changes of safety indices. Results Changes of Kt/V from baseline to 48 weeks between Changfu and Baxter showed no statistical differences; so did those of creatinine clearance rate （Ccr）. Normalized protein catabolic rate （nPCR） from baseline to 48 weeks between Changfu and Baxter showed no statistical differences; so did those of net ultrafiltration volume （nUF） and estimated glomerular filtration rate （eGFR）. Changes of nPCR from baseline to 48 weeks between 6 L and 8 L showed no statistical differences; so did those of nUF and eGFR. The decline of Kt/V from baseline to 48 weeks in 6 L group was more than that in 8 L group. Change of Ccr was similar. During the 48-week period, the mean Kt/V was above 1.7/w, and mean Ccr was above 50 L.1.73 m-2.w1. More adverse events were found in Changfu group before Changfu Corporation commenced technology optimization, and the statistical differences disappeared after that. Conclusions The domestic PD solution （Changfu） was proven to be as effective as Baxter dialysis solution. During 48- week period, a dosage of 6 L/d was enough for these patients to reach adequate PD. Clinical study promotes technological optimization, further helps to improve the safety indices of the medical products.展开更多
The peritoneum response to peritoneal dialysis can lead to fibrosis. The transforming growth factor-β1 （TGF-β1） plays an important role in regulating tissue repair and remodeling after injury. Excessive synthesis ...The peritoneum response to peritoneal dialysis can lead to fibrosis. The transforming growth factor-β1 （TGF-β1） plays an important role in regulating tissue repair and remodeling after injury. Excessive synthesis and deposition of matrix proteins by peritoneal mesothelial cells can lead to structural and functional changes in the peritoneal membrane, jeopardizing the long-term efficacy of peritoneal dialysis （PD）. Prolonged exposure to high glucose concentrations in PD fluid has been implicated as a major stimulus to matrix accumulation, through the induction of transforming growth factor-β1 （TGF-β1）.展开更多
文摘Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Methods We compared the peritoneal macrophage activity obtained from the irrigation solution of theuremia patients (n=2) immediately after inserting the catheter and with those from the long - dwell time dialysate(≥10h) of peritoneal dialysis patients (n= 7). The capacity of peritoneal macrophage to release TNF - x was assayedby L929 cytotoxicity. At the same time, we compared TNF- 7 activity among different glucose concentration (1.5%,2.5% and 4.25% dextrose) of dialysate. Results The levels of TNF- a in CAPD and control group were 170 and517 pg/106 respectively (P<0.01). The patients who were using dialysate containing higher glucose concentrationhad lower TNF- x level (P<0.05, P<0.01). Conclusion It is suggested that the inhibitory effect of peritonealdialysate on peritoneal macrophages might continue at least 10h in vivo. The higher glucose concentration ofdialysate may have worse effect on peritoneal macrophage activity.
文摘Background A multi-center large scale study is needed to confirm the efficacy and safety of domestic peritoneal dialysis （PD） solutions. Some researchers believe that 6 Lid is enough for adequate dialysis, but there is no multi-center prospective study on Chinese population to confirm this. In this study, we evaluated the efficacy and safety of domestic PD solution （Changfu） and its difference between 6 L and 8 L dosage. Methods Adult PD patients who had taken PD therapy for at least one month were selected and divided into four groups according to two dialysis solution brands and two dialysis dosages, i.e., 6 L dose with Changfu dialysis solution, 6 L dose with Baxter dialysis solution, 8 L dose with Changfu dialysis solution, and 8 L dose with Baxter dialysis solution. After 48 weeks, the changes of primary and secondary efficacy indices were compared between different types and different dosages. We also analyzed the changes of safety indices. Results Changes of Kt/V from baseline to 48 weeks between Changfu and Baxter showed no statistical differences; so did those of creatinine clearance rate （Ccr）. Normalized protein catabolic rate （nPCR） from baseline to 48 weeks between Changfu and Baxter showed no statistical differences; so did those of net ultrafiltration volume （nUF） and estimated glomerular filtration rate （eGFR）. Changes of nPCR from baseline to 48 weeks between 6 L and 8 L showed no statistical differences; so did those of nUF and eGFR. The decline of Kt/V from baseline to 48 weeks in 6 L group was more than that in 8 L group. Change of Ccr was similar. During the 48-week period, the mean Kt/V was above 1.7/w, and mean Ccr was above 50 L.1.73 m-2.w1. More adverse events were found in Changfu group before Changfu Corporation commenced technology optimization, and the statistical differences disappeared after that. Conclusions The domestic PD solution （Changfu） was proven to be as effective as Baxter dialysis solution. During 48- week period, a dosage of 6 L/d was enough for these patients to reach adequate PD. Clinical study promotes technological optimization, further helps to improve the safety indices of the medical products.
文摘The peritoneum response to peritoneal dialysis can lead to fibrosis. The transforming growth factor-β1 （TGF-β1） plays an important role in regulating tissue repair and remodeling after injury. Excessive synthesis and deposition of matrix proteins by peritoneal mesothelial cells can lead to structural and functional changes in the peritoneal membrane, jeopardizing the long-term efficacy of peritoneal dialysis （PD）. Prolonged exposure to high glucose concentrations in PD fluid has been implicated as a major stimulus to matrix accumulation, through the induction of transforming growth factor-β1 （TGF-β1）.
