The mimetic peroxidase iron-tetrakis(3-N-methylpyridyl)- porphyrin (Fe-T(3MPy)P)can be used as a catalyst in the spectrophoto- metric determination of H_2O_2 and glucose.The method is based on the formation of crystal...The mimetic peroxidase iron-tetrakis(3-N-methylpyridyl)- porphyrin (Fe-T(3MPy)P)can be used as a catalyst in the spectrophoto- metric determination of H_2O_2 and glucose.The method is based on the formation of crystal violet from leuco crystal violet.The glucose in human serum is determined by this reaction combined with catalytic reaction of glucose oxidase.展开更多
The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural enzymes.From the perspective of mimicking active site microenvironment at low cost,we herein...The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural enzymes.From the perspective of mimicking active site microenvironment at low cost,we herein report a novel histidine-functionalized graphene quantum dot(His-GQD)/hemin complex,which exhibits the highest catalytic rate for the peroxidase-based chromogenic reaction among the hemin-containing mimetics reported so far.Also,our peroxidase mimetic shows excellent tolerance to strongly acidic conditions and can function in a wide temperature range.Lineweaver-Burk plots and comprehensive electron paramagnetic resonance analysis reveal a ping-pong type catalytic mechanism for this mimetic.In addition,His-GQD/hemin demonstrates high efficiency and accuracy in detecting H2O2 and blood glucose.Our work provides an effective design of artificial enzymes for practical applications.展开更多
文摘The mimetic peroxidase iron-tetrakis(3-N-methylpyridyl)- porphyrin (Fe-T(3MPy)P)can be used as a catalyst in the spectrophoto- metric determination of H_2O_2 and glucose.The method is based on the formation of crystal violet from leuco crystal violet.The glucose in human serum is determined by this reaction combined with catalytic reaction of glucose oxidase.
基金The authors acknowledge financial support for this work from the National Key R&D Program"nanotechnology"special focus(No.2016YFA0201600)the National Natural Science Foundation of China(Nos.21422303,21573049,21872043,81602643)+1 种基金Beijing Natural Science Foundation(No.2142036)the Knowledge Innovation Program,Youth Innovation Promotion Association,and Special Program of"One Belt One Road"of CAS.The authors thank Dr.Dexing Li for the technique support and many helpful discussions for the ITC testing.
文摘The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural enzymes.From the perspective of mimicking active site microenvironment at low cost,we herein report a novel histidine-functionalized graphene quantum dot(His-GQD)/hemin complex,which exhibits the highest catalytic rate for the peroxidase-based chromogenic reaction among the hemin-containing mimetics reported so far.Also,our peroxidase mimetic shows excellent tolerance to strongly acidic conditions and can function in a wide temperature range.Lineweaver-Burk plots and comprehensive electron paramagnetic resonance analysis reveal a ping-pong type catalytic mechanism for this mimetic.In addition,His-GQD/hemin demonstrates high efficiency and accuracy in detecting H2O2 and blood glucose.Our work provides an effective design of artificial enzymes for practical applications.
