Amyotrophic lateral sclerosis(ALS)is a neuromuscular condition resulting from the progressive degeneration of motor neurons in the cortex,brainstem,and spinal cord.While the typical clinical phenotype of ALS involves ...Amyotrophic lateral sclerosis(ALS)is a neuromuscular condition resulting from the progressive degeneration of motor neurons in the cortex,brainstem,and spinal cord.While the typical clinical phenotype of ALS involves both upper and lower motor neurons,human and animal studies over the years have highlighted the potential spread to other motor and non-motor regions,expanding the phenotype of ALS.Although superoxide dismutase 1(SOD1)mutations represent a minority of ALS cases,the SOD1 gene remains a milestone in ALS research as it represents the first genetic target for personalized therapies.Despite numerous single case reports or case series exhibiting extramotor symptoms in patients with ALS mutations in SOD1(SOD1-ALS),no studies have comprehensively explored the full spectrum of extramotor neurological manifestations in this subpopulation.In this narrative review,we analyze and discuss the available literature on extrapyramidal and non-motor features during SOD1-ALS.The multifaceted expression of SOD1 could deepen our understanding of the pathogenic mechanisms,pointing towards a multidisciplinary approach for affected patients in light of new therapeutic strategies for SOD1-ALS.展开更多
Though bacteria of the radiation-resistant Deinococcus radiodurans have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to o...Though bacteria of the radiation-resistant Deinococcus radiodurans have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to oxidative stress are poorly understood. In this report, the superoxide dismutase (SOD) and catalase (CAT) activities produced by these bacteria were measured, and the change of SOD and CAT activities by 20 keV N+ beam exposure was examined. Their activities were increased by N+ beam exposure from 8x 1014 ions/cm2 to 6x1015 ions/cm2.The treatment of H2O2 and [ CHC13 +CH3 CH2OH ] and the measurement of absorption spectrum showed that the increase in SOD activity was resulted from inducible activities of MnSOD in D. radiodurans AS1.633 by N+ beam exposure . These results suggested that this bacteria possess inducible defense mechanisms against the deleterious effects of oxidization.展开更多
Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their abili...Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their ability to reduce the damaging properties of reactive oxygen species within cells and organism.This review discusses the necessary requirements for nanoparticles to have SOD activity and reveals a close relationship between catalysis on prebiotic earth and the recent SOD mimics.This review also aims to highlight the progress in the development of SOD mimicking nanoparticles.We give a broad overview of nanoparticles with SOD activity,based on their material make-up,to underline their increasing diversity.展开更多
By means of electron paramagnetic resonance(EPR), chemistry luminescence and fluorescent spectroscopy, the free radical, superoxide dismutase and water soluble lipid peroxide in tears of normal eyes(150 eyes, 100 case...By means of electron paramagnetic resonance(EPR), chemistry luminescence and fluorescent spectroscopy, the free radical, superoxide dismutase and water soluble lipid peroxide in tears of normal eyes(150 eyes, 100 cases), Moorens ulcer(9 eyes), coreal grafts rejection(16 eyes) were studied. The results showed that the spin density of the free radical was 36(±058)×1012 spins/mL tear, the content of the superoxide dismutase(SOD) was 384(±145) ng/mL tear, the opposite fluorescent density of the water soluble lipid peroxide was 12912(±1691) U/mL tear in normal tears. The normal values are 25—48×1012 spins/mL tear for free radical, 239—529 ng/mL tear for SOD, 9598—16225 U/mL tear for water soluble lipid peroxide. There are significant differences in different eyes, different sexes and different ages. The free radical and lipid peroxide are higher obviously in the tears of patients with Moorens ulcer and rejected corneal grafts, compared with those of the normal control subjects(P<001), SOD is lower evidently(P<001). The above fact shows the pathogenic mechanism of Moorens ulcer and keractoplasty rejection is significantly related to toxicities injuring effect of the free radical. These results have provided important experimental data for studying lacrimalogy and new methods for clinical diagnosis and treatrment.展开更多
The level of lipid peroxides (LPO) and copper-zinc containing superoxide dismutase (SOD-Ⅰ) in the gastric mucosa and serum was determined in 141 samples from 25 patients after con-ventional subtotal gastrectomy (11 c...The level of lipid peroxides (LPO) and copper-zinc containing superoxide dismutase (SOD-Ⅰ) in the gastric mucosa and serum was determined in 141 samples from 25 patients after con-ventional subtotal gastrectomy (11 cases of Billroth Ⅰ and 14 cases of Billroth Ⅱ) and 11 patientsafter pylorus and antroseromuscular flap preserving gastrectomy (PAFPG).Those of 11 normal subjects were examined likewise to serve as control.It was found that:(1) The average LPO levelwas much higher and the average SOD-Ⅰ level much lower in the gastric mucosa of the patientsafter conventional subtotal gastrectomy especially the Billroth Ⅱ type than in that of those afterPAFPG.(2) In 36 specimens of stump mucosa,the average LPO level was significantly higher inthe tissue around the anastomotic ring than in that of the body of the stump;no marked differ-ence of SOD-Ⅰ level between the 2 was revealed.(3) The LPO value in the stump mucosa waspositively correlated to and the SOD-Ⅰ value negatively correlated to the pH value of gastric juice.These rindings suggest that the reation of oxygen free radicals in the stump mucosa may be influ-enced by the intragastric pH or by the type of digestive continuity reconstruction,and that thereaction of oxygen free radicals especially the lipid peroxidation may play a role in the pathogenesisof the lesions in the anastomotic stoma.展开更多
The 3rd Hospital of Wuchang District ,Wuhan 432600)The squamous cell carcinoma of lung was induced with methylcholanthrene (MCA) in iodized oil in wistar rts- During the development of the cancer, the animals were giv...The 3rd Hospital of Wuchang District ,Wuhan 432600)The squamous cell carcinoma of lung was induced with methylcholanthrene (MCA) in iodized oil in wistar rts- During the development of the cancer, the animals were given superoxide dismutase (SOD) or its inhibitor diethyldio-carhamate (DDC). In DDC group, 3 out of 50 rats developed cancer and 4 developed atypical hyperplasia of hronchial epithelium within 35 to 40 days.In SOD group, no cancer developed in all of the 52 rats.and only one had atypical hyperplasia in the lungs. Only one of 42 control rats had cancer and 2 rats had atypical hyperplasia of bronchial epithelium . The difference in cancer frequency between groups DDC and SOD was significant (P<0.05). The results suggest that there is a synergism between DDC and MCA in the induction of lung cancer, while SOD can inhibit MCA-induced lung cancer development. The mechanism of the effect of SOD and DDC was discussed.展开更多
Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion...Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion of erythrocytes in 18 patients with dilated cardiomyopathy and 16 healthy controls were measured. Results Superoxide dlsmutase(SOD) activites of erythrocytes were lower in dilated cardiomyopathy(DCM) patients than that in healthy controls (P <o. oo1 ). The lipids composition of erythrocytes has changed in the DCM patients compared with healthy controls: total liplds changed little (P >o. o5); total phospholipids were lower, but not significantly (P>o. o5): total cholesterol increased significantly (P <o. o5). The cholesterol to phospholipids molecular ratio of erythrocyte membrane has increased remarkably (P >o. o5). Conclusion It can be supposed that decreased SOD activitles play an important role in the damage or membrane system and the pathogensis of DCM.展开更多
文摘Amyotrophic lateral sclerosis(ALS)is a neuromuscular condition resulting from the progressive degeneration of motor neurons in the cortex,brainstem,and spinal cord.While the typical clinical phenotype of ALS involves both upper and lower motor neurons,human and animal studies over the years have highlighted the potential spread to other motor and non-motor regions,expanding the phenotype of ALS.Although superoxide dismutase 1(SOD1)mutations represent a minority of ALS cases,the SOD1 gene remains a milestone in ALS research as it represents the first genetic target for personalized therapies.Despite numerous single case reports or case series exhibiting extramotor symptoms in patients with ALS mutations in SOD1(SOD1-ALS),no studies have comprehensively explored the full spectrum of extramotor neurological manifestations in this subpopulation.In this narrative review,we analyze and discuss the available literature on extrapyramidal and non-motor features during SOD1-ALS.The multifaceted expression of SOD1 could deepen our understanding of the pathogenic mechanisms,pointing towards a multidisciplinary approach for affected patients in light of new therapeutic strategies for SOD1-ALS.
基金This work was supported by the National Nature Science Foundation of China! No.19605005
文摘Though bacteria of the radiation-resistant Deinococcus radiodurans have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to oxidative stress are poorly understood. In this report, the superoxide dismutase (SOD) and catalase (CAT) activities produced by these bacteria were measured, and the change of SOD and CAT activities by 20 keV N+ beam exposure was examined. Their activities were increased by N+ beam exposure from 8x 1014 ions/cm2 to 6x1015 ions/cm2.The treatment of H2O2 and [ CHC13 +CH3 CH2OH ] and the measurement of absorption spectrum showed that the increase in SOD activity was resulted from inducible activities of MnSOD in D. radiodurans AS1.633 by N+ beam exposure . These results suggested that this bacteria possess inducible defense mechanisms against the deleterious effects of oxidization.
文摘Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their ability to reduce the damaging properties of reactive oxygen species within cells and organism.This review discusses the necessary requirements for nanoparticles to have SOD activity and reveals a close relationship between catalysis on prebiotic earth and the recent SOD mimics.This review also aims to highlight the progress in the development of SOD mimicking nanoparticles.We give a broad overview of nanoparticles with SOD activity,based on their material make-up,to underline their increasing diversity.
文摘By means of electron paramagnetic resonance(EPR), chemistry luminescence and fluorescent spectroscopy, the free radical, superoxide dismutase and water soluble lipid peroxide in tears of normal eyes(150 eyes, 100 cases), Moorens ulcer(9 eyes), coreal grafts rejection(16 eyes) were studied. The results showed that the spin density of the free radical was 36(±058)×1012 spins/mL tear, the content of the superoxide dismutase(SOD) was 384(±145) ng/mL tear, the opposite fluorescent density of the water soluble lipid peroxide was 12912(±1691) U/mL tear in normal tears. The normal values are 25—48×1012 spins/mL tear for free radical, 239—529 ng/mL tear for SOD, 9598—16225 U/mL tear for water soluble lipid peroxide. There are significant differences in different eyes, different sexes and different ages. The free radical and lipid peroxide are higher obviously in the tears of patients with Moorens ulcer and rejected corneal grafts, compared with those of the normal control subjects(P<001), SOD is lower evidently(P<001). The above fact shows the pathogenic mechanism of Moorens ulcer and keractoplasty rejection is significantly related to toxicities injuring effect of the free radical. These results have provided important experimental data for studying lacrimalogy and new methods for clinical diagnosis and treatrment.
文摘The level of lipid peroxides (LPO) and copper-zinc containing superoxide dismutase (SOD-Ⅰ) in the gastric mucosa and serum was determined in 141 samples from 25 patients after con-ventional subtotal gastrectomy (11 cases of Billroth Ⅰ and 14 cases of Billroth Ⅱ) and 11 patientsafter pylorus and antroseromuscular flap preserving gastrectomy (PAFPG).Those of 11 normal subjects were examined likewise to serve as control.It was found that:(1) The average LPO levelwas much higher and the average SOD-Ⅰ level much lower in the gastric mucosa of the patientsafter conventional subtotal gastrectomy especially the Billroth Ⅱ type than in that of those afterPAFPG.(2) In 36 specimens of stump mucosa,the average LPO level was significantly higher inthe tissue around the anastomotic ring than in that of the body of the stump;no marked differ-ence of SOD-Ⅰ level between the 2 was revealed.(3) The LPO value in the stump mucosa waspositively correlated to and the SOD-Ⅰ value negatively correlated to the pH value of gastric juice.These rindings suggest that the reation of oxygen free radicals in the stump mucosa may be influ-enced by the intragastric pH or by the type of digestive continuity reconstruction,and that thereaction of oxygen free radicals especially the lipid peroxidation may play a role in the pathogenesisof the lesions in the anastomotic stoma.
文摘The 3rd Hospital of Wuchang District ,Wuhan 432600)The squamous cell carcinoma of lung was induced with methylcholanthrene (MCA) in iodized oil in wistar rts- During the development of the cancer, the animals were given superoxide dismutase (SOD) or its inhibitor diethyldio-carhamate (DDC). In DDC group, 3 out of 50 rats developed cancer and 4 developed atypical hyperplasia of hronchial epithelium within 35 to 40 days.In SOD group, no cancer developed in all of the 52 rats.and only one had atypical hyperplasia in the lungs. Only one of 42 control rats had cancer and 2 rats had atypical hyperplasia of bronchial epithelium . The difference in cancer frequency between groups DDC and SOD was significant (P<0.05). The results suggest that there is a synergism between DDC and MCA in the induction of lung cancer, while SOD can inhibit MCA-induced lung cancer development. The mechanism of the effect of SOD and DDC was discussed.
文摘Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion of erythrocytes in 18 patients with dilated cardiomyopathy and 16 healthy controls were measured. Results Superoxide dlsmutase(SOD) activites of erythrocytes were lower in dilated cardiomyopathy(DCM) patients than that in healthy controls (P <o. oo1 ). The lipids composition of erythrocytes has changed in the DCM patients compared with healthy controls: total liplds changed little (P >o. o5); total phospholipids were lower, but not significantly (P>o. o5): total cholesterol increased significantly (P <o. o5). The cholesterol to phospholipids molecular ratio of erythrocyte membrane has increased remarkably (P >o. o5). Conclusion It can be supposed that decreased SOD activitles play an important role in the damage or membrane system and the pathogensis of DCM.
