目的探讨过氧化物酶6(peroxiredoxin-6,Prdx6)在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其预后意义。方法回顾性分析286例DLBCL中Prdx6的表达及与临床病理特征和预后之间的关系。结果Prdx6在DLBCL中的表达...目的探讨过氧化物酶6(peroxiredoxin-6,Prdx6)在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其预后意义。方法回顾性分析286例DLBCL中Prdx6的表达及与临床病理特征和预后之间的关系。结果Prdx6在DLBCL中的表达高于正常淋巴结(χ^(2)=17.69,P<0.001);Prdx6在non-GCB组中的表达高于GCB组(χ^(2)=21.771,P<0.001);Prdx6在有B症状病例的阳性表达强度高于无B症状病例(χ^(2)=4.872,P=0.027);Prdx6在乳酸脱氢酶(lactate dehydrogenase,LDH)升高病例中的阳性表达强度高于LDH正常病例(χ^(2)=5.965,P=0.015)。随访124例患者中,Prdx6强阳性组患者的预后比阴性组预后差(χ^(2)=6.998,P=0.008)。结论Prdx6在DLBCL中的表达较正常淋巴结高,且与non-GCB组、B症状和LDH有关。同时,Prdx6高表达与患者预后密切相关,提示可能对DLBCL的形成以及发生、发展机制具有一定作用。展开更多
Chinese forest musk deer(FMD),an endangered species,have exhibited low reproductive rates even in captivity due to stress conditions.Investigation revealed the presence of di(2-ethylhexyl)phthalate(DEHP),an environmen...Chinese forest musk deer(FMD),an endangered species,have exhibited low reproductive rates even in captivity due to stress conditions.Investigation revealed the presence of di(2-ethylhexyl)phthalate(DEHP),an environmental endocrine disruptor,in the serum and skin of captive FMDs.Feeding FMDs with maslinic acid(MA)has been observed to alleviate the stress response and improve reproductive rates,although the precise molecular mechanisms remain unclear.Therefore,this study aims to investigate the molecular mechanisms underlying the alleviation of DEHP-induced oxidative stress and cell apoptosis in primary peritubular myoid cells(PMCs)through MA intake.Primary PMCs were isolated and exposed to DEHP in vitro.The results demonstrated that DEHP significantly suppressed antioxidant levels and promoted cell apoptosis in primary PMCs.Moreover,interfering with the expression of PRDX6 was found to induce excessive reactive oxygen species(ROS)production and cell apoptosis in primary PMCs.Supplementation with MA significantly upregulated the expression of PRDX6,thereby attenuating DEHP-induced oxidative stress and cell apoptosis in primary PMCs.These findings provide a theoretical foundation for mitigating stress levels and enhancing reproductive capacity of in captive FMDs.展开更多
文摘目的探讨过氧化物酶6(peroxiredoxin-6,Prdx6)在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及其预后意义。方法回顾性分析286例DLBCL中Prdx6的表达及与临床病理特征和预后之间的关系。结果Prdx6在DLBCL中的表达高于正常淋巴结(χ^(2)=17.69,P<0.001);Prdx6在non-GCB组中的表达高于GCB组(χ^(2)=21.771,P<0.001);Prdx6在有B症状病例的阳性表达强度高于无B症状病例(χ^(2)=4.872,P=0.027);Prdx6在乳酸脱氢酶(lactate dehydrogenase,LDH)升高病例中的阳性表达强度高于LDH正常病例(χ^(2)=5.965,P=0.015)。随访124例患者中,Prdx6强阳性组患者的预后比阴性组预后差(χ^(2)=6.998,P=0.008)。结论Prdx6在DLBCL中的表达较正常淋巴结高,且与non-GCB组、B症状和LDH有关。同时,Prdx6高表达与患者预后密切相关,提示可能对DLBCL的形成以及发生、发展机制具有一定作用。
基金supported by the Fund of Sci-Tech Innovation Program of Shaanxi Academy of Forestry(No.SXLK2021-0219)the Science and Technology Project of Shaanxi Province(No.2022SF-512)the Science and Technology Innovation and Achievement Transformation Project of Experimental Demonstration Station(base)of Northwest A&F University(No.TGZX2021-32)。
文摘Chinese forest musk deer(FMD),an endangered species,have exhibited low reproductive rates even in captivity due to stress conditions.Investigation revealed the presence of di(2-ethylhexyl)phthalate(DEHP),an environmental endocrine disruptor,in the serum and skin of captive FMDs.Feeding FMDs with maslinic acid(MA)has been observed to alleviate the stress response and improve reproductive rates,although the precise molecular mechanisms remain unclear.Therefore,this study aims to investigate the molecular mechanisms underlying the alleviation of DEHP-induced oxidative stress and cell apoptosis in primary peritubular myoid cells(PMCs)through MA intake.Primary PMCs were isolated and exposed to DEHP in vitro.The results demonstrated that DEHP significantly suppressed antioxidant levels and promoted cell apoptosis in primary PMCs.Moreover,interfering with the expression of PRDX6 was found to induce excessive reactive oxygen species(ROS)production and cell apoptosis in primary PMCs.Supplementation with MA significantly upregulated the expression of PRDX6,thereby attenuating DEHP-induced oxidative stress and cell apoptosis in primary PMCs.These findings provide a theoretical foundation for mitigating stress levels and enhancing reproductive capacity of in captive FMDs.
