[Objectives]This study was conducted to establish a rapid and effective method for simultaneous extraction of 54 kinds of veterinary drug residues in animal-derived food, including sulfonamides, quinolones, tetracycli...[Objectives]This study was conducted to establish a rapid and effective method for simultaneous extraction of 54 kinds of veterinary drug residues in animal-derived food, including sulfonamides, quinolones, tetracyclines, malachite greens, penicillins, nitroimidazoles, tranquilizers and macrolides, by HPLC-MS. [Methods] The samples were extracted with 80% acetonitrile water(containing 0.1% formic acid), combined with QuEChERS extraction technology and C18 and PSA purification, analyzed by high performance liquid chromatography-mass spectrometry, and quantified by external standard method. The target substances were analyzed on ZORBAX Eclipse C18 chromatographic column using 0.2% formic acid water and 0.2% methanol as mobile phases. The gradient elution mode was used for chromatographic separation and multiple reaction detection. [Results] In the linear range of 0.5-50.0 ng/ml, the linear relationship of the 54 kinds of veterinary drug residues was good, with correlation coefficients(r~2) greater than 0.995, and the detection limits ranged from 0.30 to 1.00 μg/kg. The results showed that the recovery ranged from 75.4% to 118.2% when different matrixes were added for recovery. [Conclusions] This method is simple, efficient, accurate, stable, and highly operable. It is applicable to simultaneous batch screening of veterinary drug residues in animal-derived food, and has high practical application value.展开更多
As an important guarantee for the prevention and control of animal diseases,veterinary drugs have important functions in improving animal production performance and product quality and maintaining ecological balance.T...As an important guarantee for the prevention and control of animal diseases,veterinary drugs have important functions in improving animal production performance and product quality and maintaining ecological balance.They are an important guarantee for the healthy development of animal husbandry,food safety and public health.However,the irrational use and abuse of veterinary drugs and feed pharmaceutical additives are widespread,causing harmful substances in animal foods and damage to human health,and threatening the sustainable development of the environment and animal husbandry as well.In order to ensure human health,it is urgent to develop a simple,rapid,high-sensitivity,high-throughput and low-cost veterinary drug residue detection technology.In this paper,the sample pretreatment methods and detection techniques for the analysis of veterinary drug residues in animal foods were reviewed.展开更多
With the people's lives improving,the demands of livestock products on the import and export aspects were growing. Veterinary drug residues as the important factor which might influence animal products safety has ...With the people's lives improving,the demands of livestock products on the import and export aspects were growing. Veterinary drug residues as the important factor which might influence animal products safety has become a major concern. In this paper,the status quo and inspection of the veterinary drug residue and the corresponding control measures were put forward.展开更多
With the long -term and irrational use of veterinary drugs and drug additives in the process of animal feeding, the veterinary drug resi- dues in animals and their additives can be ingested into the human body and pos...With the long -term and irrational use of veterinary drugs and drug additives in the process of animal feeding, the veterinary drug resi- dues in animals and their additives can be ingested into the human body and pose potential threat to human health. Detection of veterinary drug resi- dues are extremely important practical significance to protect the ecological environment and human health. In recent years, liquid chromatography -tandem mass spectrometry has been widely used in vetednary drug residue detection because of its applicability, qualitative and quantitative fea- tures as well as high sensitivity. This article summarized the application of liquid chromatography -tandem mass spectrometry in detection veterinary drug residues from feed, animal products, arid udne as well as illicit drugs and harmful additives.展开更多
Thousands of tons of pharmaceutically active substances are used in veterinary medicines in intensive livestock and poultry production, but most of the drugs are poorly absorbed by animals and excreted with the animal...Thousands of tons of pharmaceutically active substances are used in veterinary medicines in intensive livestock and poultry production, but most of the drugs are poorly absorbed by animals and excreted with the animal feces. The veterinary medicines can cause various kinds of harmful impact to the environment. Now the relevant research has been a hot all over the world. With the feces excreted to the environment, the veterinary medicines can be accumulated in the soil and water, and produce all sorts of reaction. Some medicines can cause toxic effect to animals, plants and microorganisms. The article summarized the residue, the environmental impact, the transformation and the development direction of the veterinary medicine in the environment to supply frame of reference for further research.展开更多
目的了解内蒙古自治区部分地区鸡蛋中土霉素和强力霉素残留情况,并评估膳食风险。方法在内蒙古自治区部分地区的农贸市场、商店、网店等采集共180份样品,采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS...目的了解内蒙古自治区部分地区鸡蛋中土霉素和强力霉素残留情况,并评估膳食风险。方法在内蒙古自治区部分地区的农贸市场、商店、网店等采集共180份样品,采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)进行检测,分析鸡蛋中的土霉素、强力霉素残留含量,根据内蒙古居民的饮食消费情况,采用点评估法和食品安全指数法对鸡蛋中兽药的暴露风险进行评估。结果2022—2023年内蒙古自治区部分地区土霉素、强力霉素总体样本检出率为24.44%(44/180),超标率为12.22%(22/180)。强力霉素检出42份,检出率为23.33%(42/180),超标率为12.22%(22/180);土霉素检出3份,检出率为1.67%(3/180),此次调查中无超标样本;鸡蛋中强力霉素食品安全指数均值为0.001851,最大值为0.2137,均小于1。结论内蒙古自治区部分地区鸡蛋样本中存在少量的土霉素和强力霉素残留,暴露风险较低,对人体健康危害处在可接受范围。展开更多
目的:本研究建立了一种一步净化法结合液相色谱串联质谱(LC-MS/MS)同时检测雅鱼(Schizothorax prenanti)中13类54种兽药残留的快速检测方法。方法:向2.0 g样品中加入8 mL 0.2%甲酸-乙腈:水溶液(90:10,V/V)提取,冰水浴超声提取,LPAS一步...目的:本研究建立了一种一步净化法结合液相色谱串联质谱(LC-MS/MS)同时检测雅鱼(Schizothorax prenanti)中13类54种兽药残留的快速检测方法。方法:向2.0 g样品中加入8 mL 0.2%甲酸-乙腈:水溶液(90:10,V/V)提取,冰水浴超声提取,LPAS一步净化。采用C18柱,甲醇-0.1%甲酸/5 mmol/L乙酸铵溶液梯度洗脱,动态多反应监测(dMRM),正/负离子模式,内标法定量,LC-MS/MS测定。以添加54种兽药的方式,对提取方式、提取体积、盐的加入、液相和质谱条件进行了优化,对方法性能(基质效应、方法检出限、方法定量限、准确度、精密度)等进行了验证。结果:54种兽药在0.1~50μg/L范围内线性关系良好,决定系数(R^(2))≥0.992,方法检出限(LOD)为0.03~2.5μg/kg之间,方法定量限(LOQ)为0.1~8.3μg/kg之间,在低(添加量5~10μg/kg)、中(添加量10~20μg/kg)、高(添加量50μg/kg)三个水平下54种药物的平均回收率为70.45%~118.1%,RSD为0.01%~9.9%。对50份养殖雅鱼样本应用该方法进行检测,7份样品有药物检出,恩诺沙星和达氟沙星各检出2次,磺胺甲噁唑检出4次,均低于定量限的定性检出。结论:该方法操作简单,一步净化,且方法检出限低、灵敏度高,准确度和精密度均较好,方法性能验证其符合多种兽药残留要求,可为大批量检测雅鱼中多种类兽药残留提供有力支持。展开更多
文摘[Objectives]This study was conducted to establish a rapid and effective method for simultaneous extraction of 54 kinds of veterinary drug residues in animal-derived food, including sulfonamides, quinolones, tetracyclines, malachite greens, penicillins, nitroimidazoles, tranquilizers and macrolides, by HPLC-MS. [Methods] The samples were extracted with 80% acetonitrile water(containing 0.1% formic acid), combined with QuEChERS extraction technology and C18 and PSA purification, analyzed by high performance liquid chromatography-mass spectrometry, and quantified by external standard method. The target substances were analyzed on ZORBAX Eclipse C18 chromatographic column using 0.2% formic acid water and 0.2% methanol as mobile phases. The gradient elution mode was used for chromatographic separation and multiple reaction detection. [Results] In the linear range of 0.5-50.0 ng/ml, the linear relationship of the 54 kinds of veterinary drug residues was good, with correlation coefficients(r~2) greater than 0.995, and the detection limits ranged from 0.30 to 1.00 μg/kg. The results showed that the recovery ranged from 75.4% to 118.2% when different matrixes were added for recovery. [Conclusions] This method is simple, efficient, accurate, stable, and highly operable. It is applicable to simultaneous batch screening of veterinary drug residues in animal-derived food, and has high practical application value.
基金Supported by National Beef Industrial Technology System(CARS-38)Basic Science Research Fund(1610322018002)
文摘As an important guarantee for the prevention and control of animal diseases,veterinary drugs have important functions in improving animal production performance and product quality and maintaining ecological balance.They are an important guarantee for the healthy development of animal husbandry,food safety and public health.However,the irrational use and abuse of veterinary drugs and feed pharmaceutical additives are widespread,causing harmful substances in animal foods and damage to human health,and threatening the sustainable development of the environment and animal husbandry as well.In order to ensure human health,it is urgent to develop a simple,rapid,high-sensitivity,high-throughput and low-cost veterinary drug residue detection technology.In this paper,the sample pretreatment methods and detection techniques for the analysis of veterinary drug residues in animal foods were reviewed.
基金Supported by Scientific and Technological Program in State Quality Detection Bureau(2088IK013,2010IK004)Key Scientic techndogical Program(2012ZRZ014,2012SZZ030)
文摘With the people's lives improving,the demands of livestock products on the import and export aspects were growing. Veterinary drug residues as the important factor which might influence animal products safety has become a major concern. In this paper,the status quo and inspection of the veterinary drug residue and the corresponding control measures were put forward.
文摘With the long -term and irrational use of veterinary drugs and drug additives in the process of animal feeding, the veterinary drug resi- dues in animals and their additives can be ingested into the human body and pose potential threat to human health. Detection of veterinary drug resi- dues are extremely important practical significance to protect the ecological environment and human health. In recent years, liquid chromatography -tandem mass spectrometry has been widely used in vetednary drug residue detection because of its applicability, qualitative and quantitative fea- tures as well as high sensitivity. This article summarized the application of liquid chromatography -tandem mass spectrometry in detection veterinary drug residues from feed, animal products, arid udne as well as illicit drugs and harmful additives.
文摘Thousands of tons of pharmaceutically active substances are used in veterinary medicines in intensive livestock and poultry production, but most of the drugs are poorly absorbed by animals and excreted with the animal feces. The veterinary medicines can cause various kinds of harmful impact to the environment. Now the relevant research has been a hot all over the world. With the feces excreted to the environment, the veterinary medicines can be accumulated in the soil and water, and produce all sorts of reaction. Some medicines can cause toxic effect to animals, plants and microorganisms. The article summarized the residue, the environmental impact, the transformation and the development direction of the veterinary medicine in the environment to supply frame of reference for further research.
文摘目的了解内蒙古自治区部分地区鸡蛋中土霉素和强力霉素残留情况,并评估膳食风险。方法在内蒙古自治区部分地区的农贸市场、商店、网店等采集共180份样品,采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)进行检测,分析鸡蛋中的土霉素、强力霉素残留含量,根据内蒙古居民的饮食消费情况,采用点评估法和食品安全指数法对鸡蛋中兽药的暴露风险进行评估。结果2022—2023年内蒙古自治区部分地区土霉素、强力霉素总体样本检出率为24.44%(44/180),超标率为12.22%(22/180)。强力霉素检出42份,检出率为23.33%(42/180),超标率为12.22%(22/180);土霉素检出3份,检出率为1.67%(3/180),此次调查中无超标样本;鸡蛋中强力霉素食品安全指数均值为0.001851,最大值为0.2137,均小于1。结论内蒙古自治区部分地区鸡蛋样本中存在少量的土霉素和强力霉素残留,暴露风险较低,对人体健康危害处在可接受范围。
