Biological Characteristics of Marssonina coronaria and Its Pesticide Screening in Laboratory

Chaenomeles sinensis spot leaf cast is one of the most serious diseases in production. The biological characteristics of Marssonina coronaria were studied and the suitable pesticides against the pathogen were screened in the paper, to provide a scientific basis for studies on epidemic regularity and control of the disease. The effects of different nutrition and environmental conditions as well as nine pesticides on the mycelial growth of M. coronaria were investigated in this paper. The results indicated that the mycelium grew well on the PDA medium with pH of 7.0, and soluble starch and urea were suitable carbon and nitrogen sources, respec- tively. The lethal temperature was 45 ~C, and the light-dark alternation （12/12h） was benefit for mycelial growth. Among the nine tested pesticides, carbendazim was the most effective agent, with ECs0 of 8.78 mg/L, and its relative inhibition rate was 85 % under the recommended concentration of 1.0 mg/L. Mancozeb was the least effective agent, and its relative inhibition rate was only 40.8% under the recommended concentration of 1.67 mg/L.

Yield Chemical Prevention and Control Test of Asterococcus muratae Kuwana

In field chemical prevention and control test of A. muratae, effect of re- moving tertia coating method was the fastest in five pesticide application methods, and preventive effect of burying pesticide method at the root was significantly im- proved after 14 days. Corrected death rates of the two methods were both above 70%, and corrected death rate of burying pesticide method at the root reached 100% after using pesticide secondly for 7 days. For different pesticide tests, pre- vention and control effect of matrine water aqua stoste, matrine water aqua 10 times liquid and imidacloprid wet powder 10 times liquid coating was the best, and burying pesticide method at the root and pesticide liquid irrigating root method had the best prevention and control effect when respectively used imidacloprid wet pow- der and dimethoate missible oil. It was suggested to use 40-60 g/plant of imidaclo- pdd wet powder at the root during occurrence period of nymphae, which had simple operation and good effect.

Determination of eighty-two pesticides and application to screening pesticides in cannabis growing facilities

Determination of pesticides in cannabis facilities is increasingly important as medicinal and recreational uses of cannabis products expand rapidly. We report a method involving wipe sampling, liquid chromatography separation, and tandem mass spectrometry, which enables determination of 82 pesticides out of the 96 regulated by Health Canada. To demonstrate an application of the method, we sampled and measured pesticides in two cannabis growing facilities, representing a non-certified and a certified site. We detected 41 pesticides in surface wipe samples at the non-certified site and 6 at the certified site. This study provides the first evidence showing pesticide occurrence on common surfaces in cannabis growing facilities and points to a need for routine monitoring and strict control of pesticide use in cannabis facilities.