A new flavone diglycoside, named as pyrropetioside A 1 was isolated from Pyrrosia petiolosa. Its structrue was elucidated as 7-O-[6-O-(α-L-arabifuranosyl)-β-D-glucopyranosyl]-gossypetin by means of chemical and spec...A new flavone diglycoside, named as pyrropetioside A 1 was isolated from Pyrrosia petiolosa. Its structrue was elucidated as 7-O-[6-O-(α-L-arabifuranosyl)-β-D-glucopyranosyl]-gossypetin by means of chemical and spectroscopie methods including IR, MS, 1 D and 2D NMR techniques.展开更多
An efficient, sensitive, accurate and rapid analytical ultra-fast liquid chromatography （UFLC） method for quality evaluations ofPyrrosia petiolosa （Christ） Ching from 20 regions of China was developed in this stud...An efficient, sensitive, accurate and rapid analytical ultra-fast liquid chromatography （UFLC） method for quality evaluations ofPyrrosia petiolosa （Christ） Ching from 20 regions of China was developed in this study. Ten marker compounds were simultaneously quantified, including 5-caffeoylquinic acid （5-CQA）, 3-caffeoylquinic acid （3-CQA）, 4-caffeoylquinic acid （4-CQA）, 1-caffeoylquinic acid （1-CQA）, 3,5-dicaffeoylquinic acid （3,5-diCQA）, 4,5-dicaffeoylquinic acid （4,5-diCQA）, 3,4-dicaffeoylquinic acid （3,4-diCQA）, astragalin, kaempferol-3,7-di-O-glucoside and （±）eriodictyol-7-O-β-D-glucuronide. Chromatography was performed on a Kromasil 100-2.5C18 （100 mm×2.1 mm, 2.5 μm） C18 column with gradient elution. The mobile phases consisted of 0.1% formic acid/water （A） and 0.1% formic acid/methanol （B）. The detection wavelength was set at 326 nm and the flow rate was 0.4 mL/min. Ten components were separated well with good linearity （r2〉0.9998）, precision, repeatability, stability. The recovery was in the range of 99.08%-102.77%. The results showed that the content determination using RP-UFLC-DAD fingerprint technique provides an efficient, sensitive, accurate and rapid analytical method for quality assessment ofP. petiolosa （Christ） Ching. Cluster analysis and principal components analysis were successfully applied to analyze 20 samples, the results revealed that the method was efficient and authentic to distinguish producing areas and the source of P. petiolosa （Christ） Ching. Keywords： Pyrrosiapetiolosa （Christ） Ching, Caffeoylquinic acids, Flavonoids, Multicomponent determination, UFLC展开更多
目的:通过HPLC法对有柄石韦及华北石韦中绿原酸和木犀草素含量的测定,以控制石韦药材质量,建立石韦药材中绿原酸及木犀草素的含量测定方法,同时对有柄石韦及华北石韦中绿原酸及木犀草素的含量进行比较以及对不同浸提物的指纹图谱进行分...目的:通过HPLC法对有柄石韦及华北石韦中绿原酸和木犀草素含量的测定,以控制石韦药材质量,建立石韦药材中绿原酸及木犀草素的含量测定方法,同时对有柄石韦及华北石韦中绿原酸及木犀草素的含量进行比较以及对不同浸提物的指纹图谱进行分析。方法:用hypersil ODS-2柱,流动相为乙腈-0.5%磷酸水(9∶91),检测波长326 nm,流速1 m L·min-1。结果:各峰可较好的分离,有柄石韦中绿原酸的含量可达到1.51%。结论:该方法操作简便,结果准确,具有较好的重现性与稳定性,可用于有柄石韦及华北石韦中绿原酸和木犀草素含量测定。展开更多
文摘A new flavone diglycoside, named as pyrropetioside A 1 was isolated from Pyrrosia petiolosa. Its structrue was elucidated as 7-O-[6-O-(α-L-arabifuranosyl)-β-D-glucopyranosyl]-gossypetin by means of chemical and spectroscopie methods including IR, MS, 1 D and 2D NMR techniques.
基金Study of Safety Testing Techniques and Standards on New Traditional Chinese Drug(National Key Science and Technology Special Projects,Grant No.2014ZX09304307-001-001)
文摘An efficient, sensitive, accurate and rapid analytical ultra-fast liquid chromatography （UFLC） method for quality evaluations ofPyrrosia petiolosa （Christ） Ching from 20 regions of China was developed in this study. Ten marker compounds were simultaneously quantified, including 5-caffeoylquinic acid （5-CQA）, 3-caffeoylquinic acid （3-CQA）, 4-caffeoylquinic acid （4-CQA）, 1-caffeoylquinic acid （1-CQA）, 3,5-dicaffeoylquinic acid （3,5-diCQA）, 4,5-dicaffeoylquinic acid （4,5-diCQA）, 3,4-dicaffeoylquinic acid （3,4-diCQA）, astragalin, kaempferol-3,7-di-O-glucoside and （±）eriodictyol-7-O-β-D-glucuronide. Chromatography was performed on a Kromasil 100-2.5C18 （100 mm×2.1 mm, 2.5 μm） C18 column with gradient elution. The mobile phases consisted of 0.1% formic acid/water （A） and 0.1% formic acid/methanol （B）. The detection wavelength was set at 326 nm and the flow rate was 0.4 mL/min. Ten components were separated well with good linearity （r2〉0.9998）, precision, repeatability, stability. The recovery was in the range of 99.08%-102.77%. The results showed that the content determination using RP-UFLC-DAD fingerprint technique provides an efficient, sensitive, accurate and rapid analytical method for quality assessment ofP. petiolosa （Christ） Ching. Cluster analysis and principal components analysis were successfully applied to analyze 20 samples, the results revealed that the method was efficient and authentic to distinguish producing areas and the source of P. petiolosa （Christ） Ching. Keywords： Pyrrosiapetiolosa （Christ） Ching, Caffeoylquinic acids, Flavonoids, Multicomponent determination, UFLC
文摘目的:通过HPLC法对有柄石韦及华北石韦中绿原酸和木犀草素含量的测定,以控制石韦药材质量,建立石韦药材中绿原酸及木犀草素的含量测定方法,同时对有柄石韦及华北石韦中绿原酸及木犀草素的含量进行比较以及对不同浸提物的指纹图谱进行分析。方法:用hypersil ODS-2柱,流动相为乙腈-0.5%磷酸水(9∶91),检测波长326 nm,流速1 m L·min-1。结果:各峰可较好的分离,有柄石韦中绿原酸的含量可达到1.51%。结论:该方法操作简便,结果准确,具有较好的重现性与稳定性,可用于有柄石韦及华北石韦中绿原酸和木犀草素含量测定。
