Establishment of Double-antigen Sandwich Time-resolved Fluorescence Immunoassay for Detection of Pest des Petits Ruminants Virus

[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.

Establishment of High-sensitivity Rapid Fluorescence Quantitative Detection Method for Antibody against Peste des Petits Ruminants Virus

[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value.

Dulong-Petit传热规律时加热气体的最优膨胀

给定初态内能、体积,末态体积以及过程时间时对Dulong-Petit传热规律[.q∝(ΔT)5/4]下加热气体膨胀的最优构型进行了研究,利用最优控制理论得出了最大膨胀功输出时膨胀过程的最优构型由两个瞬时绝热分支和一个E-L分支组成。给出了各分支之间转换点参数的求解方法,进行最优构型的数值计算,将得到的结果与线性唯象传热规律、牛顿传热规律和辐射传热规律下加热气体膨胀的最优构型进行了比较,结果表明,虽然4种传热规律时E-L弧部分的气体内能和体积随时间的增加都是逐渐增加的,且在整个E-L弧部分温度均低于外部热槽温度,但是不同传热规律时的E-L弧的形式是不同的,初始绝热过程的终点位置也不相同,故整个膨胀过程所做出的最大功也不相同。

PourfourduPetit综合征的探讨

ＰｏｕｒｆｏｕｒｄｕＰｅｔｉｔ综合征的探讨夏惠群，刘贻德颈交感神经病损导致Ｈｏｒｎｅｒ综合征，是神经科临床工作者所熟悉的。ＰｏｕｒｆｏｕｒｄｕＰｅｔｉｔ综合征国内介绍不多，或有称之为“反Ｈｏｒｎｅｒ综合征”，或视为罕见征而不予注意。其实Ｐｏｕｒ－ｆｏ...

肺癌引起Pourfour du petit综合征1例及其红外热像表现

1病历摘要 患者,男,60岁。主诉左胸背痛伴左面部出汗增多4月余。患者于4个月前无明显诱因出现左侧胸背部疼痛,伴左侧面部出汗增多以及偶尔干咳。吸烟数十年,平均1包/日。查体：左肩胛骨内侧缘压痛（＋）,左锁骨中线第2、3肋间压痛（＋）,双肺呼吸音略粗糙,未闻及干湿性啰音。神经系统检查：左侧瞳孔直径4mm,

Monitoring and Tracking on Immune Antibody of Sheep Peste des Petits Ruminants

Peste ties petits ruminants is a kind of acute eontagious disease infecting goats anti sheep. In this study, antibtly monitoring and tracking of healthy goat and sheep immunized by peste des petits ruminants vaccine in Changping District of Beijing City were conducted, aiming at providing a reference for the devel- opment of effective immunization procedures.

Pourfour du petit综合征及其红外热像表现

Pourfour du petit综合征系因交感神经传导径路中,自下丘脑交感神经中枢往下至支配头颈上肢段任一部位刺激性病损所致。其临床表现与Horner征恰恰相反,表现为病变侧瞳孔散大、睑裂开大、眼球突出、头颈上肢或半身皮肤血管收缩以及出汗增多。

肺癌引起Pourfour du petit综合征1例

1病历摘要患者,男性,60岁。因左胸背痛伴左面部出汗增多4个月余来诊。患者于4个月前无明显诱因出现左侧胸背部疼痛,伴左侧面部出汗增多以及偶尔干咳。既往吸烟史数十年,每天约20支。查体：左肩胛骨内侧缘压痛（＋）,左锁骨中线第2、3肋间压痛（＋）,

ON THE STRICTLY POSITIVE SOLUTIONS OF NONAUTONOMOUS KOLMOGOROV COMPETITION SYSTEMS

In this paper, the existence of strictly positive solutions for N-species nonau- tonom ous Kolm ogorov com petition system s is studied. By applying the Schauder's fixed point theorem som e new sufficient conditions are established. In particular, for the alm ost periodic system , the existence of strictly positive alm ostperiodic solutions is obtained. Som e previous results are im proved and generalized.

Seroprevalence and Associated Risk Factors of Peste des Petits Ruminants among Sheep and Goats in Kassala State, Sudan

Peste des petits ruminant (PPR) is a contagious disease of small ruminants caused by a virus that belongs to the genus Morbillivirus of the family Paramyxoviridae. This study aimed to determine the seroprevalence of PPR disease in sheep and goats and its associated risk factors in Kassala State, Eastern Sudan. Across sectional study was conducted during the period from 30th August to 25th November 2015. The study was carried out using a structured questionnaire survey and a total of 918 blood samples were collected from apparently healthy unvaccinated sheep and goats in different localities in State of Kassala. A total of 546 sheep and 372 goats were tested for specific antibodies to nucleoprotein (NP) by competitive enzyme linked immunosorbent assay (cELISA). The apparent overall prevalence of PPR antibodies in Kassala was 58.2% while the true prevalence was calculated to be 61.3%. The apparent prevalence in sheep and goats was 68.1% and 43.5% respectively. Univariate analysis showed that the risk factors had significant associations with a cELISA positive status: locality, species, age, breed, husbandry system, housing mode, animals movement (p = 0.000) and animals sharing pasture and water (p = 0.003), while sex and newly introduced animals were not significant risk factors (p = 0.771) (p = 0.050) respectively. Factors found that significantly associated (p < 0.05) with increased odds of being cELISA positive in multivariate analysis were localities, species, age and newly introduced animals. The prevalence differed between localities and was the highest in the River Atbara (84.0%) locality, whereas it was lowest in Delta North (29.0%). No significant difference was observed among the sexes. However, the prevalence differed in different age groups and was 52.25% in animals of less than six months old;49.3% were between seven months and two years old and 65.5% were above two years old. In different husbandry systems, the prevalence was 47.9%, 73.0% and 49.2% in intensive, open grazing and pastoral systems respectively. Housing type effects were also observed;the highest prevalence was in animals housed in metal fence (83.3%). The movement pattern showed significant effect, where the prevalence was the highest (81.3%) in animals that move inter-states/inter-localities. It is concluded that the disease is endemic in Kassala State, high prevalent in sheep and goats, posing a threat to animal exportation, and may have a serious economic influence. Owners and herders should compulsorily vaccinate their animals yearly and animals should be investigated periodically for implementation of crucial eradication program.

Efficient and Soluble Expression of N Protein of Peste Des Petits Ruminants Virus and Development of Indirect ELISA

[ Objective] The paper was to get effective soluble N protein to establish indirect ELISA method for Peste des petits ruminants （PPR）. [ Method] Soluble N protein with high expression was obtained from Escherichia coli expression system through codon optimization and optimization of expression conditions, and indirect ELISA detection method based on N protein was further established. [ Result] The assay had no cross reaction with other sheep pathogens. The intra- and inter-batch variation coefficients were less than 9%, indicating the method had good repeatability. Furthermore, totally 480 clinical serum samples were detec- ted by the assay, and the agreement rate with commercial ELISA kit （IDVET） was 98.33%. [ Conclusion] The study laid a foundation for further development of mature PPRV antibody detection kits.

Construction of Recombinant Baculovirus Containing Peste des Petits Ruminants Virus N Gene and Establishment of Indirect ELISA for Detecting Serum Antibodies

This experiment was conducted to diagnose Peste des Petits Ruminants based on the eukaryotically-expressed PPRV nucleoprotein through an indirect ELISA. The full-length PPRV nucleoprotein gene was obtained from viral genome RNA by RT-PCR. The amplified fragments were cloned into baculovirus donor vectors pFastHTA of the Bac-to-Bac system. These recombinant plasmids, pFastHTA-PPRV-N, were transformed into DH10Bac host bacteria to obtain recombinant shuttle plasmids, pBacmid-PPRV-N. Recombinant baculovirus, Bacmid-PPRV-N, was generated for expression of the PPRV nucleoprotein by transfecting recombinant pBacmid-PPRV-N with Lipofectamine 2000 into Sf21 insect cells. The efficient expression of PPRV Nucleoprotein by baculovirus in Sf21 cells was verified by SDS-PAGE and Western blot. An indirect ELISA was developed using recombinant PPRV nucleoprotein as the coating antigen. 37 goat sera from an epidemic area in Tibet and 92 goat sera from a non-infected area in Qinghai Province were simultaneously detected by the indirect ELISA, developed here, and the international standard cELISA kit. The sensitivity and specificity of the indirect ELISA was 100% and 96.2% compared with the cELISA kit. The coincidence rate of the two methods was 96.9%. The results demonstrated that the indirect ELISA established in this study works well for diagnosis of PPR.

Sero-Prevalence and Risk Factors of Diffusion of Peste des Petits Ruminants in Cameroon

The present study was carried out between April 2015 and January 2016 to estimate the sero-prevalence and identify the risk factors of the peste des petits ruminants (PPR) in Cameroon. A total of 269 herds randomly sampled across the country have been studied and 1622 samples of serum have been levied on the sheep and goat. The c-ELISA has been studied in order to detect the presence of antibodies in small ruminants like an indicator of exposition to PPRV. The results revealed the circulation of PPRV in the country with a total sero-prevalence of 39% [95%CI;37 - 41] and a sero-prevalence of 63.2% [95%CI;57.2 - 69.2] at the herd level. Sero-prevalence was variable in the ten regions ranging from 7% [95% CI;6.2 - 8.4] to 73% [95% CI;62 - 84] with the northern zone (Adamawa, North and Far-North) having 52.3% [95% CI;37 - 60] and southern zone (including the remaining seven regions) recording 29% [95% CI;11 - 57]. Similarly, it was higher in animals found in urban/peri-urban areas than in rural areas with prevalence ratio of 2.9 [95% CI 2.54 - 3.4;p < 0.001] i.e. 3 times more, 1.6 [95% CI 1.36 - 1.90;p < 0.001] i.e. 1.6 times more, and 5.02 [95% CI 3.91 - 6.85;p < 0.001] i.e. 5 times more at national level, in the northern zone and in the southern area, respectively. Five risk factors have been identified: the breeding environment, introduction of new animals into the herds, gathering of animals for pasture and watering, wandering and transhumance. The breeding area appeared to be the most important risk factor associated with disease exposure. The control measures for the eradication of this disease must take into account the epidemiological situation, the breeding environment, animal transhumance and breeding system.

Effects of Protective Structures in Morpho-Sedimentological Changes： A Case Study of Rufisque＇s Coast, Petite C6te, Senegal

Protective structures, built in the 1980＇s in order to reduce or stop coastal erosion in Rufisque, involved a modification of the coastal morpho-sedimentary landscape and have intensified the coastal dynamics. The diachronic study of beach profiles, before and after the seawalls construction showed that these protective structures accentuated the coastal erosion, with more important sedimentary deficits at the ends of the structures. Today, the Rufisque coast is characterized by a set of sedimentary cells whose morphological evolution and annual sedimentary dynamics are controlled by seasonal forcing： sedimentary deposits in dry season and erosion in wet season. However, this alternation of deposits and erosion phase shows spatiotemporal disparities. These disparities are very marked between a northwestern area characterized by concave profiles and a southeastern area （the bay of Rufisque） characterized by convex profiles and a much more important sedimentary deficit. The results also showed that these methods of shoreline protection are not durable measurements （degradation of the structures） and have exacerbated the vulnerability to coastal erosion as well as those of the coastal populations.

TRAVELLING FRONT SOLUTION FOR A CLASS OF COMPETITION-DIFFUSION SYSTEM WITH HIGH-ORDER SINGULAR POINT

In thispaper,theexistence oftravelling frontsolution fora classofcom petition-diffu- sion system w ith high-order singular point w it = diw ixx - w αii fi(w ),x ∈R,t> 0,i= 1,2 (Ⅰ) is studied,w here di,αi> 0 (i= 1,2) and w = (w 1(x,t),w 2(x,t)).Under the certain assum ptions on f,itis show ed thatifαi< 1 for som e i,then (Ⅰ) has no travelling frontsolution,ifαi≥1 for i= 1,2,then there isa c0,c:c0≥c> 0,w herecis called the m inim alwavespeed of(Ⅰ),such thatifc≥c0 orc= c,then (Ⅰ) has a travelling frontsolution,ifc< c,then (Ⅰ) hasno travel- ling frontsolution by using the shooting m ethod in com bination w ith a com pactness argum ent.

Young Chinese Singers Take Top Prizes at World Competitions

TWENTY one-year-old Fan Chunmei,a singer from an artensemble of the Chinese People‘s Liberation Army,won firstprize in the fourth Sound of Asia International Popular SongCompetition,held in Kazakhstan on July 19.Ai Jing,from LiaoningProvince,won the Alma-Ata Mayor’s Prize.She sang“My 1997.”Yuan Chenyie of the China Opera Theater took second prize inthe Classical division of the Tokyo International Vocal Music Com-petition,held in Tokyo in June this year.

不用喂食的机器宠物Petit Qoobo猫尾机器人

准确地说,这是一只没有头、没有脚,只有半截身子和猫尾的半只“猫”。Petit Qoobo猫尾机器人主要是模拟真猫在乖巧的时候,近贴着主人爬着,摇动尾巴时的场景,这种动作是表明猫很信任主人,让主人抚摸它,它自己会感觉很舒服。它外表毛茸茸,采用仿宠物绒毛的材质,可以让你随便“撸”、放开“吸”,并且不需要担心过敏和受伤。它就像一块靠垫,你可以抱着它、抚摸它甚至躺在上边,尽情地感受这毛茸茸的舒服感,实足的治愈系机器人。

专业社会工作赋能信访问题治理的理论基础与实践路径

引入社会力量参与信访问题治理是做好新时代信访工作的关键举措。专业社会工作凭借其理论基础和实务方法,成为赋能信访矛盾善治的重要结构性主体。本文从价值、理论、方法三个维度研讨专业社会工作赋能信访工作何以可能的理论问题,阐释专业社会工作在开展信访问题源头治理、以专业识别与干预技术赋能、推进信访矛盾全过程治理等方面的特殊治理效能。在此基础上,描摹和展望了专业社会工作赋能信访问题治理的实践路径,包括嵌入基层治理组织体系、入驻乡镇(街道)社会工作站、承接政府发包项目等。实现专业社会工作赋能信访问题治理效能的跃升,还必须注重信访社会工作的职业化与专业化发展,促进行政性社会工作与专业性社会工作的嵌合,加强党建引领并明确信访社工的角色定位,真正将专业社会工作塑造为新时代信访工作转换升级的重要依凭。

无理涉诉信访治理中适用听证制度的路径思考——基于H省L市法院信访案件处理的实证分析

无理涉诉信访是当前困扰各级法院的重大难题,它具有信访层级向上偏好、线上途径占比较高、重复访现象普遍、信访终结难度大等特征,并在实践中主要呈现谋利型、偏执型和复合型三种典型形态。无理涉诉信访严重损害司法公信力、浪费社会资源、破坏诚信体系,必须在法治轨道内进行治理。在涉诉信访治理中适用听证制度具有特殊法治意义,它有助于保障当事人理性表达、促进信息公开、形成认知共识,进而实现程序正义。现阶段存在听证制度适用标准不明确、适用程序不规范、配套措施不完善等问题,应明确听证制度的启用标准、优化听证的重点环节、深化听证结果运用,强化无理信访惩戒等配套制度,从而提升无理涉诉信访处理的效能和法治化水平,促进信访治理现代化。