[Objectives]This study was conducted to establish a microscopic identification method of Polygonum capitatum Buch.Ham.ex D.Don.[Methods]The cross sections were identified by microscopic identification.[Results]The ste...[Objectives]This study was conducted to establish a microscopic identification method of Polygonum capitatum Buch.Ham.ex D.Don.[Methods]The cross sections were identified by microscopic identification.[Results]The stem cross section of P.capitatum is round-like,and shows pericyclic fibers forming a ring,strongly lignified,many vascular bundles,and a hollow pith part.There are many starch granules in the powder,and single granules are more common;and fibers are mostly bundled or scattered,lignified or non-lignified,and calcium oxalate cluster crystals are common.There are many pollen grains,obtuse triangular or round-like,and some of them have three germination apertures.They have fine thorn-like protrusions on the outer wall,the surface of which has reticulate carvings.[Conclusions]The results of microscopic identification are reliable and can be used as the basis for identification of P.capitatum.展开更多
目的以网络药理学研究结果为基础,对小建中汤中桂枝、甘草、生姜、大枣、白芍、饴糖6种中药开展深入研究,以期为小建中汤治疗脾胃虚寒类消化性溃疡作用机制研究提供新的依据及方向。方法基于中药系统药理学数据库与分析平台(TCMSP)、中...目的以网络药理学研究结果为基础,对小建中汤中桂枝、甘草、生姜、大枣、白芍、饴糖6种中药开展深入研究,以期为小建中汤治疗脾胃虚寒类消化性溃疡作用机制研究提供新的依据及方向。方法基于中药系统药理学数据库与分析平台(TCMSP)、中医药综合数据库(TCMID)获取小建中汤6种中草药的相关化学成分,并在Swiss Target Prediction中搜索活性化学成分潜在靶点;通过GeneCards数据库检索消化性溃疡、十二指肠溃疡、胃溃疡靶点和脾胃虚寒型消化性溃疡主要症状靶点;筛选出疾病主要症状、疾病和药物的共同靶点;运用Cytoscape Version 3.7.2软件绘制药物-化合物-靶点网络;利用STRING数据库绘制构建蛋白质相互作用(PPI)网络分析共同靶点之间的相互作用,找出可能的关键基因;利用R软件进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)富集分析,分析治疗的作用机制;运用AutoDock及Pymol对关键靶点与相应活性成分进行分子对接验证,实验验证小建中汤对靶点蛋白、白细胞介素(IL)-6表达的影响。结果分析得到小建中汤具有128个活性化学成分作用于75个脾胃虚寒型消化性溃疡靶点,其中TP53、血管内皮生长因子(VEGF)、IL-6、胱天蛋白酶3(CASP3)、丝氨酸/苏氨酸蛋白激酶1(AKT1)、信号转导因子和转录激活因子3(STAT3)、丝裂原活化蛋白激酶3(MAPK3)、白蛋白(ALB)、表皮生长因子受体(EGFR)与非受体酪氨酸激酶(SRC)为核心靶点;而小建中汤治疗脾胃虚寒型消化性溃疡的核心活性成分是儿茶素、羟黄酮、芍药内苷C、拟雌内酯、草果甲素等;靶点基因主要富集于脂筏、膜微结构域、膜区、细胞小凹等细胞区域,作用于跨膜受体蛋白酪氨酸激酶活性、蛋白酪氨酸激酶活性、跨膜受体蛋白激酶活性、磷酸酶结合等分子功能,重要的是具有调节平滑肌细胞增殖和氧化应激反应。生物学通路则主要集中于磷脂酰肌醇3-激酶(PI3K)-Akt信号通路、缺氧诱导因子(HIF)-1信号通路、C型凝集素受体信号通路、胃癌等通路。分子对接结果显示,筛选的主要核心成分与其对应靶蛋白具有较好的结合活性。结论小建中汤通过网络药理学预测的作用机制多成分、多靶点、多通路治疗脾胃虚寒型消化性溃疡;实验验证小建中汤可下调靶点蛋白IL-6的表达,抑制肠上皮损伤。展开更多
The roots of O. fragrans are also a valuable resource in addition to its flowers and fruits. In this study, the HPLC-MS/MS method used for analyzing the chemical constituents in O. fragrans roots extract was developed...The roots of O. fragrans are also a valuable resource in addition to its flowers and fruits. In this study, the HPLC-MS/MS method used for analyzing the chemical constituents in O. fragrans roots extract was developed, which showed high sensitivity for both qualitative and quantitative analyses. Thirty-two compounds were first discovered in O. fragrans roots, one compound of which was reported for the first time. The simultaneous determination method for acteoside, isoacteoside, oleuropein and phillyrin was validated to be sensitive and accurate. Then it was applied to determine the content of bioactive components in O. fragrans roots from different cultivars. The content of oleuropein and phillyrin in the twelve batches was relatively stable, while the content of acteoside and isoacteoside varied greatly.Moreover, the therapeutic material basis and mechanism of O. fragrans roots exerting its traditional pharmacodynamics were analyzed by network pharmacology. The results showed that O. fragrans roots might be effective for the treatment of inflammation, cardiovascular diseases, cancer, and rheumatoid arthritis, which is consistent with the traditional pharmacodynamics of O. fragrans roots. This work can provide an analytical method for the comprehensive development of O. fragrans roots.展开更多
OBJECTIVE Our group mainly focuses on the target identification and pharmacological mechanism study of TCM.We deeply identified the direct targets of the active ingredients in TCM using molecule probe-'Target Fish...OBJECTIVE Our group mainly focuses on the target identification and pharmacological mechanism study of TCM.We deeply identified the direct targets of the active ingredients in TCM using molecule probe-'Target Fishing' technology in chemical biology,and explored the related signaling pathways to explain the traditional efficiency of TCM.METHODS We synthesized biotin-tagged mole.cule probe by connecting biotin tag to TCM active molecule using PGE as a linker.Then,the biotintagged molecule probe was bound to the surface of solid beads by strong biotin-avidin interaction.Thus,the molecule probe-bound beads were mixed with cell lysates to capture the potential targets and identified by MS.RESULTS Our study found that SA which was an anti-inflammatory compound.could selectively bind to IMPDH2 in microglial cells,and SA showed weaker anti-inflammatory effect on IMPDH2-knock down microglial cells,suggesting IMPDH2 as a key anti-inflammatory target for SA.Ad.ditionally,handelin was a key anti-inflammatory compound.We identified the target protein of handelin as Hsp70 from microglial cells using target pull-down technology.Moreover,handelin showed weaker anti-inflammatory effect on Hsp70-knock down microglial cells,revealing that Hsp70 was the direct antiinflammatory target of handelin.CONCLUSION Our study provided methodology references for TCM target identification in the future,and also showed a new insight for exploring the pharmacological mechanism of TCM active ingredients.More importantly,we can perform scientific annotation for TCM efficiency by clarifying the biological functions of each target protein,showing important significance on modernization and internationalization of TCM.展开更多
基金Supported by Guangxi Key Research and Development Project(GK AB19110027)High-level Innovation Teams and Outstanding Scholars Program of Colleges and Universities in Guangxi:Zhuang Medicine Basic and Clinical Research Innovation Team(GJR[2014]07)The 2018 Guangxi First-class Discipline Construction Project of Guangxi University of Chinese Medicine(2018XK056)。
文摘[Objectives]This study was conducted to establish a microscopic identification method of Polygonum capitatum Buch.Ham.ex D.Don.[Methods]The cross sections were identified by microscopic identification.[Results]The stem cross section of P.capitatum is round-like,and shows pericyclic fibers forming a ring,strongly lignified,many vascular bundles,and a hollow pith part.There are many starch granules in the powder,and single granules are more common;and fibers are mostly bundled or scattered,lignified or non-lignified,and calcium oxalate cluster crystals are common.There are many pollen grains,obtuse triangular or round-like,and some of them have three germination apertures.They have fine thorn-like protrusions on the outer wall,the surface of which has reticulate carvings.[Conclusions]The results of microscopic identification are reliable and can be used as the basis for identification of P.capitatum.
文摘目的以网络药理学研究结果为基础,对小建中汤中桂枝、甘草、生姜、大枣、白芍、饴糖6种中药开展深入研究,以期为小建中汤治疗脾胃虚寒类消化性溃疡作用机制研究提供新的依据及方向。方法基于中药系统药理学数据库与分析平台(TCMSP)、中医药综合数据库(TCMID)获取小建中汤6种中草药的相关化学成分,并在Swiss Target Prediction中搜索活性化学成分潜在靶点;通过GeneCards数据库检索消化性溃疡、十二指肠溃疡、胃溃疡靶点和脾胃虚寒型消化性溃疡主要症状靶点;筛选出疾病主要症状、疾病和药物的共同靶点;运用Cytoscape Version 3.7.2软件绘制药物-化合物-靶点网络;利用STRING数据库绘制构建蛋白质相互作用(PPI)网络分析共同靶点之间的相互作用,找出可能的关键基因;利用R软件进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)富集分析,分析治疗的作用机制;运用AutoDock及Pymol对关键靶点与相应活性成分进行分子对接验证,实验验证小建中汤对靶点蛋白、白细胞介素(IL)-6表达的影响。结果分析得到小建中汤具有128个活性化学成分作用于75个脾胃虚寒型消化性溃疡靶点,其中TP53、血管内皮生长因子(VEGF)、IL-6、胱天蛋白酶3(CASP3)、丝氨酸/苏氨酸蛋白激酶1(AKT1)、信号转导因子和转录激活因子3(STAT3)、丝裂原活化蛋白激酶3(MAPK3)、白蛋白(ALB)、表皮生长因子受体(EGFR)与非受体酪氨酸激酶(SRC)为核心靶点;而小建中汤治疗脾胃虚寒型消化性溃疡的核心活性成分是儿茶素、羟黄酮、芍药内苷C、拟雌内酯、草果甲素等;靶点基因主要富集于脂筏、膜微结构域、膜区、细胞小凹等细胞区域,作用于跨膜受体蛋白酪氨酸激酶活性、蛋白酪氨酸激酶活性、跨膜受体蛋白激酶活性、磷酸酶结合等分子功能,重要的是具有调节平滑肌细胞增殖和氧化应激反应。生物学通路则主要集中于磷脂酰肌醇3-激酶(PI3K)-Akt信号通路、缺氧诱导因子(HIF)-1信号通路、C型凝集素受体信号通路、胃癌等通路。分子对接结果显示,筛选的主要核心成分与其对应靶蛋白具有较好的结合活性。结论小建中汤通过网络药理学预测的作用机制多成分、多靶点、多通路治疗脾胃虚寒型消化性溃疡;实验验证小建中汤可下调靶点蛋白IL-6的表达,抑制肠上皮损伤。
基金supported by the National Natural Science Foundation of China (Grant Nos. 81872828 and 81573384)the Large-scale Instrument and Equipment Sharing Foundation of Wuhan University (LF20191065)。
文摘The roots of O. fragrans are also a valuable resource in addition to its flowers and fruits. In this study, the HPLC-MS/MS method used for analyzing the chemical constituents in O. fragrans roots extract was developed, which showed high sensitivity for both qualitative and quantitative analyses. Thirty-two compounds were first discovered in O. fragrans roots, one compound of which was reported for the first time. The simultaneous determination method for acteoside, isoacteoside, oleuropein and phillyrin was validated to be sensitive and accurate. Then it was applied to determine the content of bioactive components in O. fragrans roots from different cultivars. The content of oleuropein and phillyrin in the twelve batches was relatively stable, while the content of acteoside and isoacteoside varied greatly.Moreover, the therapeutic material basis and mechanism of O. fragrans roots exerting its traditional pharmacodynamics were analyzed by network pharmacology. The results showed that O. fragrans roots might be effective for the treatment of inflammation, cardiovascular diseases, cancer, and rheumatoid arthritis, which is consistent with the traditional pharmacodynamics of O. fragrans roots. This work can provide an analytical method for the comprehensive development of O. fragrans roots.
基金supported by National Natural Science Foundation of China(81773932)
文摘OBJECTIVE Our group mainly focuses on the target identification and pharmacological mechanism study of TCM.We deeply identified the direct targets of the active ingredients in TCM using molecule probe-'Target Fishing' technology in chemical biology,and explored the related signaling pathways to explain the traditional efficiency of TCM.METHODS We synthesized biotin-tagged mole.cule probe by connecting biotin tag to TCM active molecule using PGE as a linker.Then,the biotintagged molecule probe was bound to the surface of solid beads by strong biotin-avidin interaction.Thus,the molecule probe-bound beads were mixed with cell lysates to capture the potential targets and identified by MS.RESULTS Our study found that SA which was an anti-inflammatory compound.could selectively bind to IMPDH2 in microglial cells,and SA showed weaker anti-inflammatory effect on IMPDH2-knock down microglial cells,suggesting IMPDH2 as a key anti-inflammatory target for SA.Ad.ditionally,handelin was a key anti-inflammatory compound.We identified the target protein of handelin as Hsp70 from microglial cells using target pull-down technology.Moreover,handelin showed weaker anti-inflammatory effect on Hsp70-knock down microglial cells,revealing that Hsp70 was the direct antiinflammatory target of handelin.CONCLUSION Our study provided methodology references for TCM target identification in the future,and also showed a new insight for exploring the pharmacological mechanism of TCM active ingredients.More importantly,we can perform scientific annotation for TCM efficiency by clarifying the biological functions of each target protein,showing important significance on modernization and internationalization of TCM.
