We report a novel stimulated Raman scattering(SRS)microscopy technique featuring phase-controlled light focusing and aberration corrections for rapid,deep tissue 3D chemical imaging with subcellular resolution.To acco...We report a novel stimulated Raman scattering(SRS)microscopy technique featuring phase-controlled light focusing and aberration corrections for rapid,deep tissue 3D chemical imaging with subcellular resolution.To accomplish phasecontrolled SRS(PC-SRS),we utilize a single spatial light modulator to electronically tune the axial positioning of both the shortened-length Bessel pump and the focused Gaussian Stokes beams,enabling z-scanning-free optical sectioning in the sample.By incorporating Zernike polynomials into the phase patterns,we simultaneously correct the system aberrations at two separate wavelengths(~240 nm difference),achieving a~3-fold enhancement in signal-to-noise ratio over the uncorrected imaging system.PC-SRS provides>2-fold improvement in imaging depth in various samples(e.g.,polystyrene bead phantoms,porcine brain tissue)as well as achieves SRS 3D imaging speed of~13 Hz per volume for real-time monitoring of Brownian motion of polymer beads in water,superior to conventional point-scanning SRS 3D imaging.We further utilize PC-SRS to observe the metabolic activities of the entire tumor liver in living zebrafish in cellsilent region,unraveling the upregulated metabolism in liver tumor compared to normal liver.This work shows that PCSRS provides unprecedented insights into morpho-chemistry,metabolic and dynamic functioning of live cells and tissue in real-time at the subcellular level.展开更多
In view of the problem of fine characterization of narrow and thin channels,the maximum entropy criterion is used to enhance the focusing characteristics of Wigner-Ville Distribution.On the basis of effectively improv...In view of the problem of fine characterization of narrow and thin channels,the maximum entropy criterion is used to enhance the focusing characteristics of Wigner-Ville Distribution.On the basis of effectively improving the time-frequency resolution of seismic signal,a new method of microscopic ancient river channel identification is established.Based on the principle of the equivalence between the maximum entropy power spectrum and the AR model power spectrum,the prediction error and the autoregression coefficient of AR model are obtained using the Burg algorithm and Levinson-Durbin recurrence rule.Under the condition of the first derivative of autocorrelation function being 0,the Wigner-Ville Distribution of seismic signal is calculated,and the Wigner-Ville Distribution time-frequency power spectrum(MEWVD)is obtained under the maxi-mum entropy criterion of the microscopic ancient river channel.Through analysis of emulational seismic signal and forward numerical simulation signal of narrow thin model,it is found that MEWVD can effectively avoid the interference of cross term of Wigner-Ville Distribution,and obtain more accurate spectral characteristics than STFT and CWT signal analysis methods.It is also proved that the narrow and thin river channels of different scales can be identified effectively by MEWVD of different frequencies.The method is applied to the third member of Jurassic Shaximiao Formation(J2s33-2)gas reservoir of the Zhongji-ang gas field in Sichuan Basin.The spatial information of width and direction of narrow and thin river channels with width less than 500 m and sandstone thickness less than 35 m is accurately identified,providing bases for well deployment and horizontal well fracturing section selection.展开更多
基金supported by the Academic Research Fund(AcRF)from the Ministry of Education(MOE)(Tier 2(A-8000117-01-00)Tier 1(R397-000-334-114,R397-000-371-114,and R397-000-378-114)2024 Tsinghua-NUS Joint Research Initiative Fund,and the National Medical Research Council(NMRC)(A-0009502-01-00,and A-8001143-00-00),Singapore.
文摘We report a novel stimulated Raman scattering(SRS)microscopy technique featuring phase-controlled light focusing and aberration corrections for rapid,deep tissue 3D chemical imaging with subcellular resolution.To accomplish phasecontrolled SRS(PC-SRS),we utilize a single spatial light modulator to electronically tune the axial positioning of both the shortened-length Bessel pump and the focused Gaussian Stokes beams,enabling z-scanning-free optical sectioning in the sample.By incorporating Zernike polynomials into the phase patterns,we simultaneously correct the system aberrations at two separate wavelengths(~240 nm difference),achieving a~3-fold enhancement in signal-to-noise ratio over the uncorrected imaging system.PC-SRS provides>2-fold improvement in imaging depth in various samples(e.g.,polystyrene bead phantoms,porcine brain tissue)as well as achieves SRS 3D imaging speed of~13 Hz per volume for real-time monitoring of Brownian motion of polymer beads in water,superior to conventional point-scanning SRS 3D imaging.We further utilize PC-SRS to observe the metabolic activities of the entire tumor liver in living zebrafish in cellsilent region,unraveling the upregulated metabolism in liver tumor compared to normal liver.This work shows that PCSRS provides unprecedented insights into morpho-chemistry,metabolic and dynamic functioning of live cells and tissue in real-time at the subcellular level.
基金Supported by the General Project of National Natural Science Foundation(4207416041574099)the Sichuan Science and Tech-nology Plan Project(2020JDRC0013)。
文摘In view of the problem of fine characterization of narrow and thin channels,the maximum entropy criterion is used to enhance the focusing characteristics of Wigner-Ville Distribution.On the basis of effectively improving the time-frequency resolution of seismic signal,a new method of microscopic ancient river channel identification is established.Based on the principle of the equivalence between the maximum entropy power spectrum and the AR model power spectrum,the prediction error and the autoregression coefficient of AR model are obtained using the Burg algorithm and Levinson-Durbin recurrence rule.Under the condition of the first derivative of autocorrelation function being 0,the Wigner-Ville Distribution of seismic signal is calculated,and the Wigner-Ville Distribution time-frequency power spectrum(MEWVD)is obtained under the maxi-mum entropy criterion of the microscopic ancient river channel.Through analysis of emulational seismic signal and forward numerical simulation signal of narrow thin model,it is found that MEWVD can effectively avoid the interference of cross term of Wigner-Ville Distribution,and obtain more accurate spectral characteristics than STFT and CWT signal analysis methods.It is also proved that the narrow and thin river channels of different scales can be identified effectively by MEWVD of different frequencies.The method is applied to the third member of Jurassic Shaximiao Formation(J2s33-2)gas reservoir of the Zhongji-ang gas field in Sichuan Basin.The spatial information of width and direction of narrow and thin river channels with width less than 500 m and sandstone thickness less than 35 m is accurately identified,providing bases for well deployment and horizontal well fracturing section selection.
