A new carrier containing three hydrophobic octadecyls was employed to improve the yield and purity of the synthesized oligosaccharides, and the applicability of hydrophobic carrier for HASP （hydrophobically assisted ...A new carrier containing three hydrophobic octadecyls was employed to improve the yield and purity of the synthesized oligosaccharides, and the applicability of hydrophobic carrier for HASP （hydrophobically assisted switching phase） was also explored. High performance liquid chromatography （HPLC） was used to analyze the purity of oligosaccharides and improve the elution efficiency. Elution system was optimized and replaced by isopropyl alcohol and water, in which the excess donor and other impurities had a better solubility than that in methanol.展开更多
基金supported by the grants from the National Natural Science Foundation of China(No. 21232002)the National Basic Research Program of China(No. 2012CB822100)
文摘A new carrier containing three hydrophobic octadecyls was employed to improve the yield and purity of the synthesized oligosaccharides, and the applicability of hydrophobic carrier for HASP （hydrophobically assisted switching phase） was also explored. High performance liquid chromatography （HPLC） was used to analyze the purity of oligosaccharides and improve the elution efficiency. Elution system was optimized and replaced by isopropyl alcohol and water, in which the excess donor and other impurities had a better solubility than that in methanol.
